LOCUS       40924_45788        5453 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pURYL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Lv H, Gong Z.
  TITLE     Cost-effective and repeatable gene targeting in fission yeast using 
            a cloning-based and modular method
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Lv H, Gong Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 5453)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5453)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAR-2012) School of Life Science, Fudan University, Room 618, 
            Science Building, Handan Road 220, Shanghai 200433, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             292..1002
                     /label=mEYFP
                     /note="enhanced YFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
     CDS             1003..1020
                     /label=6xHis
                     /note="6xHis affinity tag"
     regulatory      1030..1522
                     /label=Saccharomyces cerevisiae Leu2 terminator
                     /note="Saccharomyces cerevisiae Leu2 terminator"
                     /regulatory_class="terminator"
     regulatory      1529..2050
                     /label=Schizosaccharomyces pombe Ura4 promoter
                     /note="Schizosaccharomyces pombe Ura4 promoter"
                     /regulatory_class="promoter"
     CDS             2051..2842
                     /label=S. pombe ura4
                     /note="orotidine 5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      2846..3275
                     /label=Schizosaccharomyces pombe Ura4 terminator
                     /note="Schizosaccharomyces pombe Ura4 terminator"
                     /regulatory_class="terminator"
     promoter        complement(3342..3360)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3618..4206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4380..5237)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5238..5342)
                     /label=AmpR promoter
ORIGIN
        1 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
       61 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      121 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt
      181 gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
      241 gtgacactat agaacgcggc cgccagctga agcttctcga gccccggatc cagtaaagga
      301 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      361 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      421 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      481 ggttatggtc taaaatgctt tgctagatac ccagatcata tgaaacggca tgactttttc
      541 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      601 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      661 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
      721 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
      781 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
      841 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcctatcaa
      901 tctaagcttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
      961 acagctgctg ggattacaca tggcatggat gaactataca aacaccatca ccatcaccat
     1021 taatctagag aaaatccttg cttaaaaaga ttctcttttt ttatgatatt tgtacataaa
     1081 ctttataaat gaaattcata atagaaacga cacgaaatta caaaatggaa tatgttcata
     1141 gggtagacga aactatatac gcaatctaca tacatttatc aagaaggaga aaaaggagga
     1201 tgtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa ggaaaaagaa
     1261 ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt taggtgtaac
     1321 agaaaatcat gaaactatga ttcctaattt atatattgga ggattttctc taaaaaaaaa
     1381 aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt taagtcaata
     1441 ccttcttgaa ccatttccca taatggtgaa agttccctca agaattttac tctgtcagaa
     1501 acggccttaa cgacgtagtc gaagatctca aatcccactg gctatatgta tgcatttgtg
     1561 ttaaaaaagt ttgtatagat tatttaatct actcagcatt ctttctctaa ataggaattt
     1621 gttacttaat ggagaaaaaa atgtttcgat ttacctagtg tatttgtttg tatactcacg
     1681 tttaatttca aacatccatt ctatcttgtg taatttttgg catggtgaaa aagataatca
     1741 gccttataat ctttacaaaa gtaagaaatt ctgtaaataa gccttaatgc ccttgcttta
     1801 aattaaaatg gttctttttc atgataatgt ttgcactttg tgaatatatt ttagatagtt
     1861 ctgtgaggta taattaagat gttttagaga cttatacaat tttgtcttta taaattctta
     1921 attgatttta ccatcccagt ttaactatgc ttcgtcggca tctctgcaca tgtcgtgttt
     1981 tcttaccgta ttgtcctacc aagaacctct tttttgcttg gatcgaaatt aaaggtttaa
     2041 aagcaaagtt atggatgcta gagtatttca aagctattca gctagagctg aggggatgaa
     2101 aaatcccatt gccaaggaat tgttggcttt gatggaagaa aagcaaagca acttgtcagt
     2161 cgcggtcgat ttgacgaaga aatccgaaat cttagaattg gtagataaaa ttggacccta
     2221 tgtctgtgtt atcaagacac atattgacgt tgtcgaggat ttcgaccagg atatggtaga
     2281 aaaactggtg gccttaggta aaaagcatcg ttttcttatc tttgaggatc gcaaattcgc
     2341 agacattgga aataccgtca agctacaata tgcatctggt gtgtacaaaa ttgcttcttg
     2401 ggctcatatc acaaattgcc atacagtgcc aggcgagggt attatacagg gcctcaaaga
     2461 agttggttta cctttgggac gtggtctctt gcttttggct gaaatgtctt ccaaaggctc
     2521 tttggctact ggttcctaca cagagaaaac cttagaatgg tttgagaagc ataccgattt
     2581 ttgctttggc tttatagctg gtcgtcgatt tcctaacctt caaagcgact acataactat
     2641 gtcccctggt atcggcttgg atgttaaagg agacgggctg ggacagcaat atcgtactcc
     2701 tgaagaagtg attgtaaact gcggtagcga tatcatcatt gttggtcgtg gagtctatgg
     2761 agctggtcgt aatcctgttg tcgaagccaa gagatataga gaagctggtt ggaaggcata
     2821 tcagcaaaga ctttctcagc attaaaaaaa gactaatgta aaattttttt ggttggttat
     2881 tgaaaaagtc gatgccttgt ttgcgtttgt tttcctaggc gttttatgtc agaaggcatt
     2941 tagaattagt atacaagtac tctttggtaa aattttatgt agcgactaaa atattaacta
     3001 ttatagataa acaccttggg aataaaaagt aatttgctat agtaatttat taaacatgct
     3061 cctacaacat taccacaatc ttttctcttg gattgacatt gaataagaaa agagtgaatt
     3121 tttttagact tgtaatgata actatgtaca aagccaatga aagatgtatg tagatgaatg
     3181 taaaatacca tgtagacaaa caagataaaa cttggttata aacattggtg ttggaacaga
     3241 ataaattaga tgtcaaaaag tttcgtcaat atcacgagct cgaattcatc gatgatatca
     3301 gatccactag tggcctatgc ggccgcggat ctgccggtct ccctatagtg agtcgtatta
     3361 atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3421 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3481 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3541 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3601 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3661 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3721 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3781 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     3841 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3901 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3961 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4021 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     4081 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4141 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4201 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4261 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4321 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4381 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4441 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4501 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4561 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4621 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4681 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4741 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4801 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4861 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4921 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4981 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5041 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5101 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5161 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5221 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5281 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5341 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     5401 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tga
//
