Basic Vector Information
- Vector Name:
- pUNI(Amp)-GFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3417 bp
- Type:
- Cloning vector
- Replication origin:
- R6K γ ori
- Source/Author:
- Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ.
pUNI(Amp)-GFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUNI(Amp)-GFP vector Sequence
LOCUS 40924_45768 3417 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pUNI(Amp)-GFP, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3417)
AUTHORS Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ.
TITLE Univector plasmid-fusion system
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3417)
AUTHORS Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ.
TITLE Direct Submission
JOURNAL Submitted (10-MAY-1999) Biochemistry, Baylor College of Medicine,
One Baylor Plaza, Houston, TX 77030, USA
REFERENCE 3 (bases 1 to 3417)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3417)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(10-MAY-1999) Biochemistry, Baylor College of Medicine, One Baylor
Plaza, Houston, TX 77030, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3417
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind complement(127..160)
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
misc_feature 197..203
/label=GFP epitope tag cassette
/note="GFP epitope tag cassette"
CDS 217..927
/codon_start=1
/label=GFP (S65T)
/note="S65T variant of Aequorea victoria green fluorescent
protein (Heim et al., 1995)"
/translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
VTAAGITHGMDELYK"
misc_feature 907..1024
/label=polylinker region cassette
/note="polylinker region cassette"
polyA_signal 1044..1251
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
terminator 1323..1370
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin complement(1466..1854)
/direction=LEFT
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
rep_origin complement(1869..2324)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(2361..3218)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3219..3323)
/label=AmpR promoter
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