LOCUS       40924_45768        3417 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUNI(Amp)-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ.
  TITLE     Univector plasmid-fusion system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-1999) Biochemistry, Baylor College of Medicine, 
            One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 3417)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3417)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-1999) Biochemistry, Baylor College of Medicine, One Baylor 
            Plaza, Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(127..160)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    197..203
                     /label=GFP epitope tag cassette
                     /note="GFP epitope tag cassette"
     CDS             217..927
                     /codon_start=1
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    907..1024
                     /label=polylinker region cassette
                     /note="polylinker region cassette"
     polyA_signal    1044..1251
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      1323..1370
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1466..1854)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     rep_origin      complement(1869..2324)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(2361..3218)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3219..3323)
                     /label=AmpR promoter
ORIGIN
        1 gctagctgtc cgaaatatta taaattatcg cacacataaa aaccatgctg ttggtgtgtc
       61 tattaaatcg attttttgtt ataacagaca ctgcttgtcc gatatttgat ttaggataca
      121 ggtaccataa cttcgtatag catacattat acgaagttat ctggaattcc ccgggctcga
      181 gaacatatgg ccatggggat ccccgggtta attaacagta aaggagaaga acttttcact
      241 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      301 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      361 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcactta tggtgttcaa
      421 tgcttttcaa gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc
      481 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
      541 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
      601 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
      661 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
      721 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
      781 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
      841 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
      901 acacatggca tggatgaact atacaaatag ggcgcgccac ttctaaataa gcggccgccg
      961 agatcatatc actgtggacg ttgatgaaag aatacgttat tctttcatca aatcgtggtc
     1021 gacgagctcg ctatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     1081 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     1141 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     1201 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg attctagaag
     1261 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     1321 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     1381 gaactatatc cggatatcat gcatcgcgag agagagagag agagagagag agagagagag
     1441 agagagagag acgtgggccc aattctgtca gccgttaagt gttcctgtgt cactgaaaat
     1501 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac
     1561 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
     1621 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
     1681 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
     1741 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga
     1801 aacatgagag cttagtacgt actatcaaca ggttgaactg ctgatcaaca gatcctctac
     1861 actagtctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     1921 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     1981 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     2041 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     2101 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     2161 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     2221 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     2281 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     2341 gctgcacgcg tttcgaatta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2581 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2641 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2701 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2761 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2821 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2881 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2941 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3001 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3061 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3241 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3301 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     3361 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgt
//
