pUNI(Amp)-AD vector (V002279)

MSDS

Basic Vector Information

Vector Name:: pUNI(Amp)-AD

Antibiotic Resistance:: Ampicillin

Length:: 3028 bp

Type:: Cloning vector

Replication origin:: R6K γ ori

Source/Author:: Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ.

pUNI(Amp)-AD vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUNI(Amp)-AD vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45763        3028 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUNI(Amp)-AD, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ.
  TITLE     Univector plasmid-fusion system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-1999) Biochemistry, Baylor College of Medicine, 
            One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 3028)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3028)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-1999) Biochemistry, Baylor College of Medicine, One Baylor 
            Plaza, Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(127..160)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    197..203
                     /note="yeast GAL4 transcription activation domain cassette"
     CDS             217..558
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     polyA_signal    655..862
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      934..981
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1077..1465)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     rep_origin      complement(1480..1935)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(1972..2829)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2830..2934)
                     /label=AmpR promoter

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