LOCUS       40924_45763        3028 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUNI(Amp)-AD, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ.
  TITLE     Univector plasmid-fusion system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Liu Q, Li MZ, Leibham D, Cortez D, Elledge SJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-1999) Biochemistry, Baylor College of Medicine, 
            One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 3028)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3028)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-1999) Biochemistry, Baylor College of Medicine, One Baylor 
            Plaza, Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(127..160)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    197..203
                     /note="yeast GAL4 transcription activation domain cassette"
     CDS             217..558
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     polyA_signal    655..862
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      934..981
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1077..1465)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     rep_origin      complement(1480..1935)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(1972..2829)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2830..2934)
                     /label=AmpR promoter
ORIGIN
        1 gctagctgtc cgaaatatta taaattatcg cacacataaa aaccatgctg ttggtgtgtc
       61 tattaaatcg attttttgtt ataacagaca ctgcttgtcc gatatttgat ttaggataca
      121 ggtaccataa cttcgtatag catacattat acgaagttat ctggaattcc ccgggctcga
      181 gaacatatgg ccatggggat ccccgggtta attaacgcca attttaatca aagtgggaat
      241 attgctgata gctcattgtc cttcactttc actaacagta gcaacggtcc gaacctcata
      301 acaactcaaa caaattctca agcgctttca caaccaattg cctcctctaa cgttcatgat
      361 aacttcatga ataatgaaat cacggctagt aaaattgatg atggtaataa ttcaaaacca
      421 ctgtcacctg gttggacgga ccaaactgcg tataacgcgt ttggaatcac tacagggatg
      481 tttaatacca ctacaatgga tgatgtatat aactatctat tcgatgatga agatacccca
      541 ccaaacccaa aaaaagagta agcggccgcc gagatcatat cactgtggac gttgatgaaa
      601 gaatacgtta ttctttcatc aaatcgtggt cgacgagctc gctatcagcc tcgactgtgc
      661 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
      721 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
      781 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
      841 acaatagcag gcatgctggg gattctagaa gatccggctg ctaacaaagc ccgaaaggaa
      901 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      961 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatca tgcatcgcga
     1021 gagagagaga gagagagaga gagagagaga gagagagaga gacgtgggcc caattctgtc
     1081 agccgttaag tgttcctgtg tcactgaaaa ttgctttgag aggctctaag ggcttctcag
     1141 tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt
     1201 atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag ttgctgattt
     1261 atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
     1321 ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac atgagagctt
     1381 agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg tactatcaac
     1441 aggttgaact gctgatcaac agatcctcta cactagtcta aattgtaagc gttaatattt
     1501 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     1561 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     1621 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     1681 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     1741 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     1801 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     1861 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     1921 cgctacaggg cgcgtcccat tcgccattca ggctgcacgc gtttcgaatt accaatgctt
     1981 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2041 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2101 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2161 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2221 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2281 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2341 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2401 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2461 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2521 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2581 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2641 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2701 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2761 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2821 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     2881 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2941 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     3001 aaataggcgt atcacgaggc cctttcgt
//
