Basic Vector Information
- Vector Name:
- pUMRI-12
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6866 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Xie W, Lv X, Yu H.
- Promoter:
- TEF
pUMRI-12 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUMRI-12 vector Sequence
LOCUS 40924_45743 6866 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pUMRI-12, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6866)
AUTHORS Xie W, Lv X, Yu H.
TITLE pUMRI plasmids for metabolic pathway assembly
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6866)
AUTHORS Xie W, Lv X, Yu H.
TITLE Direct Submission
JOURNAL Submitted (21-JUL-2014) Department of Chemical and Biological
Engineering, Zhejiang University, Institute of Bioengineering, 34
TianMuShan Road, Hangzhou, Zhejiang 310028, China
REFERENCE 3 (bases 1 to 6866)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6866)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(21-JUL-2014) Department of Chemical and Biological Engineering,
Zhejiang University, Institute of Bioengineering, 34 TianMuShan
Road, Hangzhou, Zhejiang 310028, China"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Assembly Method :: Artificial Sequence v. Artificial Sequence
Sequencing Technology :: Artificial Sequence
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..6866
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 22..55
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
CDS complement(202..771)
/gene="PTH1"
/label=PTH1
/note="Peptidyl-tRNA hydrolase from Saccharomyces
cerevisiae (strain ATCC 204508 / S288c). Accession#:
P38876"
misc_feature complement(796..1499)
/label=ERG9 region
/note="ERG9 region"
regulatory complement(1497..2613)
/label=HXT1 promoter from Saccharomyces cerevisiae
/note="HXT1 promoter from Saccharomyces cerevisiae"
/regulatory_class="promoter"
regulatory 2614..3121
/label=GAL7 promoter from Saccharomyces cerevisiae
/note="GAL7 promoter from Saccharomyces cerevisiae"
/regulatory_class="promoter"
promoter 3129..3147
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3165..3194
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
terminator 3224..3413
/label=CYC1 terminator
/note="transcription terminator for CYC1"
promoter complement(3426..3444)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature complement(3467..3500)
/label=Loxp site
/note="Loxp site"
protein_bind complement(3467..3500)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
gene 3546..4902
/label=kanMX
/note="yeast selectable marker conferring kanamycin
resistance (Wach et al., 1994)"
promoter 4997..5217
/label=URA3 promoter
CDS 5218..6018
/label=URA3
/note="orotidine-5'-phosphate decarboxylase, required for
uracil biosynthesis"
rep_origin complement(6190..6778)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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