LOCUS       V002282                 6866 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002282
VERSION     V002282
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Xie W, Lv X, Yu H.
  TITLE     pUMRI plasmids for metabolic pathway assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   Xie W, Lv X, Yu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2014) Department of Chemical and Biological
            Engineering, Zhejiang University, Institute of Bioengineering, 34
            TianMuShan Road, Hangzhou, Zhejiang 310028, China
REFERENCE   3  (bases 1 to 6866)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6866)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Artificial Sequence v. Artificial Sequence
            Sequencing Technology :: Artificial Sequence
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-JUL-2014) Department of Chemical and Biological Engineering,
            Zhejiang University, Institute of Bioengineering, 34 TianMuShan
            Road, Hangzhou, Zhejiang 310028, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..55
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(202..771)
                     /gene="PTH1"
                     /label="Peptidyl-tRNA hydrolase"
                     /note="Peptidyl-tRNA hydrolase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P38876"
     misc_feature    complement(796..1499)
                     /label="ERG9 region"
                     /note="ERG9 region"
     regulatory      complement(1497..2613)
                     /label="HXT1 promoter from Saccharomyces cerevisiae"
                     /note="HXT1 promoter from Saccharomyces cerevisiae"
                     /regulatory_class="promoter"
     regulatory      2614..3121
                     /label="GAL7 promoter from Saccharomyces cerevisiae"
                     /note="GAL7 promoter from Saccharomyces cerevisiae"
                     /regulatory_class="promoter"
     promoter        3129..3147
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3165..3194
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     terminator      3224..3413
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(3426..3444)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(3467..3500)
                     /label="Loxp site"
                     /note="Loxp site"
     protein_bind    complement(3467..3500)
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     gene            3546..4902
                     /label="kanMX"
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        4997..5217
                     /label="URA3 promoter"
     CDS             5218..6018
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(6190..6778)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ggatcttcga gaacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt
       61 gatatcagat ccacggatca ctaaagggaa caaaagctgg agctggcctt gtaggccgcc
      121 ttccgatgca aagtgcagtg gaaaagaaaa agggcaaagc aaataggatg gtaagtcggt
      181 attgttgttg aagatgggct atgaaatgta ctgagtcaga gcacgccagg cagcaggttc
      241 actctgtgta agcaaggttt gtagttcctg cggagttaga gctcccagaa cccaccggga
      301 cacgctcgca gggtctctag aacgggaccc aggttctctg ccgattccaa tagccaattt
      361 ggcaaagggt acacggcctc cactgcattt tagcaggctt cgcagcccat tatgacctct
      421 aatactggtg ctgggggctc tgagctgcac ttttccacac gccacactta actcgtcatg
      481 aataactacg tgtcgggctt ggtacttggc gtaccgggcc cagaccttgc acacctgttc
      541 tccacttaga ttcatgtact ggccatccga tcttagtatc gtacaatgtt cgtcttctat
      601 ataatgcact ttgccgcccg tattaggcac aggggaataa gttctcccct gcagaccaag
      661 ccgctttcgt agcagctcca gcatatatag ccctacattg tgacgggtac cagcgtactg
      721 aggctctgga ttgcctatcc cggtcagcac tagtctccat ttaccggaca tcttccttat
      781 ctgcctagag tcgaccaaat cttcattgta atctctgatg atgttggttt tttgtaggaa
      841 aagacccatg ctttcataca attgctcatt agaatacaaa gattcgttgg caaacttggc
      901 aatgacaatc aaacgggtca aaccatcacc gaccaaacca gctacgtagt gacagtacac
      961 gtcgtagtcg tggacggttt gcaacccatt caagttgtaa ttttcatcta agatgtagtc
     1021 ggccatacca ttacccattt tctcggtgat ctccttgatg acttcttgat attctggttt
     1081 caatttgtgg aattcaataa gaatcgattc gaaatctgtc aaaacggctc tgtccttcac
     1141 atcgggggca tttccgtcga aactccattt agttaacaac aatttctcgt ggaagtgacg
     1201 caacaagtca attttcaaat cgtgttcgat ggacatatcg tcttcgatgg tatccaaagc
     1261 ccttaaaatc aaataaaaga gagtaacaca gtttctcaat tctggatgca gctctctgat
     1321 cacagcagca aacgatctgg aggtcaagtt caacagttcg aaacagtgca agagatatgg
     1381 agacgtggac tgatcataga tggagaatag cggtgttctg caaaacttca gcttcaaagc
     1441 tgccttcatc tcgaccggat gcaatgccaa ttgtaatagc tttcccatgc ggccgcgatt
     1501 ttacgtatat caactagttg acgattatga tatctttata gattttaagg tggggaaaga
     1561 acatgagacc ccagatggaa ttgattatgg ggacattgtt gcctttatat ataatttcaa
     1621 tatactaatt caaatgatta aaaacgtgag ggggacacgc aacttcgggt gttaagaaat
     1681 attttgctac attagataat ggtggagttt cctggcttgt cggataaaag ccatcaaatg
     1741 tcgcagcagc tcatgtttac gtttgctgtc ttctgcccac gtcatatgag tggtattctt
     1801 ctatcagcac ttgatgaata ttctttttct catatatctg aaagacaaaa gatcggcacg
     1861 gcaatgccct gcagcatttc ttcctagttt ttccgaattt ccattacgta ttggatcttg
     1921 tgcgcatatt tgtcagtcct tcacggaaaa aaaaaaagag cactgggtca cttcggaaaa
     1981 acttttgact caatgcaaca gtgtcataat cctttgcgct gtctctttga agaaaaatca
     2041 ggagtgcaag atatcgatta attccttgga agttatgatg gttagtctta gtttaactct
     2101 cttgaagaag ggttttttca gttggtcaac actctttaga ggtaaaaaaa aaaaaaaaaa
     2161 aaaaaaaaag agaattcttc atgtaattta ccatgattct acgtttttgc aagcaaaaat
     2221 gaagataatc cgagcgcatg cgcaagtagt ccctgccatg ccgcttcgga aaaactttcg
     2281 aaaccaatac tccttacatc cgccttgaat ttaggaccta caattgttgt ctcttctgct
     2341 tgaaccaata agccctagaa accttacacc ctaatttgca caagaaaact acgaaaaaat
     2401 tttttcctct tttttccgca gctccaatct cgccctgcta tttttttggt tcggagaaaa
     2461 atagtcggtc gatatgaatt gaagtttcat tgtggccact cgtttaggaa tggaataacc
     2521 tccgcgaatg gaaaaaaaga actcggaata tcaaaaattc cggctcaaag gaaaaatttg
     2581 cttcaggagg ggggaattat attccagatg agaaagatat ggacggtagc aacaagaata
     2641 tagcacgagc cgcggagttc atttcgttac ttttgatatc actcacaact attgcgaagc
     2701 gcttcagtga aaaaatcata aggaaaagtt gtaaatatta ttggtagtat tcgtttggta
     2761 aagtagaggg ggtaattttt cccctttatt ttgttcatac attcttaaat tgctttgcct
     2821 ctccttttgg aaagctatac ttcggagcac tgttgagcga aggctcatta gatatatttt
     2881 ctgtcatttt ccttaaccca aaaataaggg aaagggtcca aaaagcgctc ggacaactgt
     2941 tgaccgtgat ccgaaggact ggctatacag tgttcacaaa atagccaagc tgaaaataat
     3001 gtgtagctat gttcagttag tttggctagc aaagatataa aagcaggtcg gaaatattta
     3061 tgggcattat tatgcagagc atcaacatga taaaaaaaaa cagttgaata ttccctcaaa
     3121 aggatccgta atacgactca ctatagggcc cgggcgtcga catggaacag aagttgattt
     3181 ccgaagaaga cctcgagtaa gcttggtacc gcggctagct aagatccgct ctaaccgaaa
     3241 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag
     3301 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg
     3361 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aagatcccaa
     3421 ttcgccctat agtgagtcgt attacgcgcg ctcgacaacc cttaatataa cttcgtataa
     3481 tgtatgctat acgaagttat taggtctagt agcttgcctc gtccccgccg ggtcacccgg
     3541 ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg
     3601 catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat
     3661 ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct
     3721 gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt
     3781 agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta
     3841 aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca tgggtaagga
     3901 aaagactcac gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     3961 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa
     4021 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     4081 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     4141 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gcaaaacagc
     4201 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     4261 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     4321 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     4381 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt
     4441 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     4501 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     4561 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     4621 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     4681 gctcgatgag tttttctaat cagtactgac aataaaaaga ttcttgtttt caagaacttg
     4741 tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt
     4801 tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta
     4861 atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa
     4921 cgccgccatc cagtgtcgaa aacgaacagt gtggctgtgg ttcagattgt actgagagtg
     4981 caccatacca cagcttttca attcaattca tcattttttt tttattcttt tttttgattt
     5041 cggtttcttt gaaatttttt tgattcggta atctccgaac agaaggaaga acgaaggaag
     5101 gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac atgaaattgc
     5161 ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag ataaatcatg
     5221 tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc tgccaagcta
     5281 tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc
     5341 aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact aaaaacacat
     5401 gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa ggcattatcc
     5461 gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg taatacagtc
     5521 aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat tacgaatgca
     5581 cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga agaagtaaca
     5641 aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc cctatctact
     5701 ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt tgttatcggc
     5761 tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt gattatgaca
     5821 cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag aaccgtggat
     5881 gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt tgcaaaggga
     5941 agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc atatttgaga
     6001 agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat actaaactca
     6061 caaattagag cttcaattta attatatcag ttattaccct atgcggtgtg aaataccgca
     6121 cagatgcgta aggagaaaat accgcatcag gaaattgtaa acgttatttc tataatgatt
     6181 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6241 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6301 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6361 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6421 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6481 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6541 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6601 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     6661 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6721 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6781 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6841 gattttggtc atgagattat caaaaa
//
