Basic Vector Information
pUDE172 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUDE172 vector Sequence
LOCUS 40924_45438 12865 bp DNA circular SYN 18-DEC-2018 DEFINITION Expression vector pUDE172, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12865) AUTHORS Koopman F, Beekwilder J, Crimi B, Houwelingen A, Hall RD, Bosch D, van Maris AJ, Pronk JT, Daran JM. TITLE De novo production of the flavonoid naringenin in engineered Saccharomyces cerevisiae JOURNAL Microb. Cell Fact. 11 (1), 155 (2012) PUBMED 23216753 REFERENCE 2 (bases 1 to 12865) AUTHORS Koopman F, Beekwilder J, Crimi B, van Houwelingen A, Hall RD, Bosch D, Maris AJA., Pronk JT, Daran J-M. TITLE Direct Submission JOURNAL Submitted (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600, The Netherlands REFERENCE 3 (bases 1 to 12865) TITLE Direct Submission REFERENCE 4 (bases 1 to 12865) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Microb. Cell Fact."; date: "2012"; volume: "11"; issue: "1"; pages: "155" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600, The Netherlands" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..12865 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 8..651 /label=GAP promoter /note="promoter for glyceraldehyde-3-phosphate dehydrogenase; also known as the TDH3 promoter" CDS 657..2831 /gene="PAL1" /label=PAL1 /note="Phenylalanine ammonia-lyase 1 from Arabidopsis thaliana. Accession#: P35510" terminator 2835..3082 /label=CYC1 terminator /note="transcription terminator for CYC1" misc_feature 3090..3169 /label=recombination site B /note="recombination site B" promoter 3571..4078 /label=TPI1 promoter /note="strong constitutive promoter for yeast triose phosphate isomerase" CDS 4078..5592 /gene="CYP73A5" /label=CYP73A5 /note="Trans-cinnamate 4-monooxygenase from Arabidopsis thaliana. Accession#: P92994" terminator 5734..5921 /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" misc_feature 6063..6142 /label=recombination site C /note="recombination site C" regulatory 6146..6520 /label=PGI1 promoter /note="PGI1 promoter" /regulatory_class="promoter" CDS 6527..8602 /gene="ATR1" /label=ATR1 /note="NADPH--cytochrome P450 reductase 1 from Arabidopsis thaliana. Accession#: Q9SB48" regulatory 8611..8877 /operon="PGI1 terminator" /regulatory_class="terminator" misc_feature 8917..8996 /label=recombination site D /note="recombination site D" CDS complement(9129..9929) /label=URA3 /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" promoter complement(9930..10150) /label=URA3 promoter misc_feature complement(10415..10918) /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" promoter 10955..11059 /label=AmpR promoter CDS 11060..11917 /label=AmpR /note="beta-lactamase" rep_origin 12091..12679 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 12784..12863 /label=recombination site A /note="recombination site A"
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