pUDE172 vector (V002344)

Basic Vector Information

      • Vector Name:
      • pUDE172
      • Length:
      • 12865 bp
      • Type:
      • Expression vector
      • Source/Author:
      • Koopman F, Beekwilder J, Crimi B, Houwelingen A, Hall RD, Bosch D, van Maris AJ, Pronk JT, Daran JM.

pUDE172 vector Vector Map

pUDE17212865 bp6001200180024003000360042004800540060006600720078008400900096001020010800114001200012600GAP promoterPAL1CYC1 terminatorrecombination site BTPI1 promoterCYP73A5ADH1 terminatorrecombination site CPGI1 promoterATR1recombination site DURA3URA3 promoterCEN/ARSAmpR promoterAmpRorirecombination site A

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUDE172 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45438       12865 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUDE172, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Koopman F, Beekwilder J, Crimi B, Houwelingen A, Hall RD, Bosch D, 
            van Maris AJ, Pronk JT, Daran JM.
  TITLE     De novo production of the flavonoid naringenin in engineered 
            Saccharomyces cerevisiae
  JOURNAL   Microb. Cell Fact. 11 (1), 155 (2012)
  PUBMED    23216753
REFERENCE   2  (bases 1 to 12865)
  AUTHORS   Koopman F, Beekwilder J, Crimi B, van Houwelingen A, Hall RD, Bosch 
            D, Maris AJA., Pronk JT, Daran J-M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600,
            The Netherlands
REFERENCE   3  (bases 1 to 12865)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12865)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb. 
            Cell Fact."; date: "2012"; volume: "11"; issue: "1"; pages: "155"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600, The 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..651
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             657..2831
                     /gene="PAL1"
                     /label=PAL1
                     /note="Phenylalanine ammonia-lyase 1 from Arabidopsis
                     thaliana. Accession#: P35510"
     terminator      2835..3082
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     misc_feature    3090..3169
                     /label=recombination site B
                     /note="recombination site B"
     promoter        3571..4078
                     /label=TPI1 promoter
                     /note="strong constitutive promoter for yeast triose
                     phosphate isomerase"
     CDS             4078..5592
                     /gene="CYP73A5"
                     /label=CYP73A5
                     /note="Trans-cinnamate 4-monooxygenase from Arabidopsis 
                     thaliana. Accession#: P92994"
     terminator      5734..5921
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    6063..6142
                     /label=recombination site C
                     /note="recombination site C"
     regulatory      6146..6520
                     /label=PGI1 promoter
                     /note="PGI1 promoter"
                     /regulatory_class="promoter"
     CDS             6527..8602
                     /gene="ATR1"
                     /label=ATR1
                     /note="NADPH--cytochrome P450 reductase 1 from Arabidopsis 
                     thaliana. Accession#: Q9SB48"
     regulatory      8611..8877
                     /operon="PGI1 terminator"
                     /regulatory_class="terminator"
     misc_feature    8917..8996
                     /label=recombination site D
                     /note="recombination site D"
     CDS             complement(9129..9929)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(9930..10150)
                     /label=URA3 promoter
     misc_feature    complement(10415..10918)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        10955..11059
                     /label=AmpR promoter
     CDS             11060..11917
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      12091..12679
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    12784..12863
                     /label=recombination site A
                     /note="recombination site A"

This page is informational only.