Basic Vector Information
- Vector Name:
- pUDE065
- Antibiotic Resistance:
- Ampicillin
- Length:
- 12576 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Koopman F, Beekwilder J, Crimi B, Houwelingen A, Hall RD, Bosch D, van Maris AJ, Pronk JT, Daran JM.
- Promoter:
- GPD
pUDE065 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUDE065 vector Sequence
LOCUS 40924_45418 12576 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pUDE065, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12576)
AUTHORS Koopman F, Beekwilder J, Crimi B, Houwelingen A, Hall RD, Bosch D,
van Maris AJ, Pronk JT, Daran JM.
TITLE De novo production of the flavonoid naringenin in engineered
Saccharomyces cerevisiae
JOURNAL Microb. Cell Fact. 11 (1), 155 (2012)
PUBMED 23216753
REFERENCE 2 (bases 1 to 12576)
AUTHORS Koopman F, Beekwilder J, Crimi B, van Houwelingen A, Hall RD, Bosch
D, Maris AJA., Pronk JT, Daran J-M.
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600,
The Netherlands
REFERENCE 3 (bases 1 to 12576)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 12576)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Microb.
Cell Fact."; date: "2012"; volume: "11"; issue: "1"; pages: "155"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600, The
Netherlands"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT Name: pUDI065.
FEATURES Location/Qualifiers
source 1..12576
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(8..1099)
/label=LEU2
/note="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
promoter complement(1112..1516)
/label=LEU2 promoter
primer_bind 2043..2059
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 2069..2087
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
terminator complement(2102..2299)
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
CDS complement(2361..4043)
/gene="4CL3"
/label=4CL3
/note="4-coumarate--CoA ligase 3 from Arabidopsis thaliana.
Accession#: Q9S777"
promoter complement(4059..4460)
/label=TEF1 promoter
/note="promoter for EF-1-alpha"
primer_bind 4735..4751
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 4758..4776
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 5200..5708
/label=TPI1 promoter
/note="strong constitutive promoter for yeast triose
phosphate isomerase"
CDS 5713..6897
/gene="CHS"
/label=CHS
/note="Chalcone synthase from Arabidopsis thaliana.
Accession#: P13114"
terminator 6907..7094
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
promoter 7247..7891
/label=GAP promoter
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase; also known as the TDH3 promoter"
CDS 7909..8646
/gene="CHI1"
/label=CHI1
/note="Chalcone--flavanone isomerase 1 from Arabidopsis
thaliana. Accession#: P41088"
terminator 8662..8909
/label=CYC1 terminator
/note="transcription terminator for CYC1"
promoter complement(8946..8964)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(8985..9001)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 9009..9025
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9033..9063)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(9078..9099)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(9387..9975)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10149..11006)
/label=AmpR
/note="beta-lactamase"
promoter complement(11007..11111)
/label=AmpR promoter
misc_feature 11253..11964
/note="partial PYK2 gene; for integration into the PYK2
locus"
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