LOCUS       40924_45418       12576 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUDE065, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Koopman F, Beekwilder J, Crimi B, Houwelingen A, Hall RD, Bosch D, 
            van Maris AJ, Pronk JT, Daran JM.
  TITLE     De novo production of the flavonoid naringenin in engineered 
            Saccharomyces cerevisiae
  JOURNAL   Microb. Cell Fact. 11 (1), 155 (2012)
  PUBMED    23216753
REFERENCE   2  (bases 1 to 12576)
  AUTHORS   Koopman F, Beekwilder J, Crimi B, van Houwelingen A, Hall RD, Bosch 
            D, Maris AJA., Pronk JT, Daran J-M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600,
            The Netherlands
REFERENCE   3  (bases 1 to 12576)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12576)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb. 
            Cell Fact."; date: "2012"; volume: "11"; issue: "1"; pages: "155"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600, The 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     Name: pUDI065.
FEATURES             Location/Qualifiers
     source          1..12576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..1099)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(1112..1516)
                     /label=LEU2 promoter
     primer_bind     2043..2059
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2069..2087
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2102..2299)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(2361..4043)
                     /gene="4CL3"
                     /label=4CL3
                     /note="4-coumarate--CoA ligase 3 from Arabidopsis thaliana.
                     Accession#: Q9S777"
     promoter        complement(4059..4460)
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     4735..4751
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4758..4776
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5200..5708
                     /label=TPI1 promoter
                     /note="strong constitutive promoter for yeast triose
                     phosphate isomerase"
     CDS             5713..6897
                     /gene="CHS"
                     /label=CHS
                     /note="Chalcone synthase from Arabidopsis thaliana.
                     Accession#: P13114"
     terminator      6907..7094
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        7247..7891
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             7909..8646
                     /gene="CHI1"
                     /label=CHI1
                     /note="Chalcone--flavanone isomerase 1 from Arabidopsis 
                     thaliana. Accession#: P41088"
     terminator      8662..8909
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(8946..8964)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8985..9001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9009..9025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9033..9063)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9078..9099)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9387..9975)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10149..11006)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(11007..11111)
                     /label=AmpR promoter
     misc_feature    11253..11964
                     /note="partial PYK2 gene; for integration into the PYK2
                     locus"
ORIGIN
        1 ctttttaagc aaggattttc ttaacttctt cggcgacagc atcaccgact tcggtggtac
       61 tgttggaacc acctaaatca ccagttctga tacctgcatc caaaaccttt ttaactgcat
      121 cttcaatggc cttaccttct tcaggcaagt tcaatgacaa tttcaacatc attgcagcag
      181 acaagatagt ggcgataggg tcaaccttat tctttggcaa atctggagca gaaccgtggc
      241 atggttcgta caaaccaaat gcggtgttct tgtctggcaa agaggccaag gacgcagatg
      301 gcaacaaacc caaggaacct gggataacgg aggcttcatc ggagatgata tcaccaaaca
      361 tgttgctggt gattataata ccatttaggt gggttgggtt cttaactagg atcatggcgg
      421 cagaatcaat caattgatgt tgaaccttca atgtagggaa ttcgttcttg atggtttcct
      481 ccacagtttt tctccataat cttgaagagg ccaaaacatt agctttatcc aaggaccaaa
      541 taggcaatgg tggctcatgt tgtagggcca tgaaagcggc cattcttgtg attctttgca
      601 cttctggaac ggtgtattgt tcactatccc aagcgacacc atcaccatcg tcttcctttc
      661 tcttaccaaa gtaaatacct cccactaatt ctctgacaac aacgaagtca gtacctttag
      721 caaattgtgg cttgattgga gataagtcta aaagagagtc ggatgcaaag ttacatggtc
      781 ttaagttggc gtacaattga agttctttac ggatttttag taaaccttgt tcaggtctaa
      841 cactaccggt accccattta ggaccaccca cagcacctaa caaaacggca tcaaccttct
      901 tggaggcttc cagcgcctca tctggaagtg ggacacctgt agcatcgata gcagcaccac
      961 caattaaatg attttcgaaa tcgaacttga cattggaacg aacatcagaa atagctttaa
     1021 gaaccttaat ggcttcggct gtgatttctt gaccaacgtg gtcacctggc aaaacgacga
     1081 tcttcttagg ggcagacata ggggcagaca ttagaatggt atatccttga aatatatata
     1141 tatattgctg aaatgtaaaa ggtaagaaaa gttagaaagt aagacgattg ctaaccacct
     1201 attggaaaaa acaataggtc cttaaataat attgtcaact tcaagtattg tgatgcaagc
     1261 atttagtcat gaacgcttct ctattctata tgaaaagccg gttccggcct ctcacctttc
     1321 ctttttctcc caatttttca gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa
     1381 caaaaaattt ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt
     1441 atgttgagga aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct
     1501 gagtattccc acagttaact gcggtcaaga tatttcttga atcaggcgcc ttagaccgct
     1561 cggccaaaca accaattact tgttgagaaa tagagtataa ttatcctata aatataacgt
     1621 ttttgaacac acatgaacaa ggaagtacag gacaattgat tttgaagaga atgtggattt
     1681 tgatgtaatt gttgggattc catttttaat aaggcaataa tattaggtat gtggatatac
     1741 tagaagttct cctcgagggt cgattgcggg gaagcggcga cgtggcgaga aaggaaggga
     1801 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     1861 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     1921 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     1981 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     2041 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     2101 ccagtatagc gaccagcatt cacatacgat tgacgcatga tattactttc tgcgcactta
     2161 acttcgcatc tgggcagatg atgtcgaggc gaaaaaaaat ataaatcacg ctaacatttg
     2221 attaaaatag aacaactaca atataaaaaa actatacaaa tgacaagttc ttgaaaacaa
     2281 gaatcttttt attgtcagta ctgctcgagc ggccgccagt gtgatggata tctgcagaat
     2341 tcgcccttgc ggtcgactca acaaagctta gctttgaggt cctttcttag tatttttccc
     2401 gaaggagatt taggaatgga agcaacaaag aagaccttgt gcaatctctt atagaacacc
     2461 acctgtttgg caacatattc tttaacatct tcttcagtga tatcatttcc atttgatcgc
     2521 accacgaaag ccaccggaac ttccccagcg acttcatcat tttgcggaac aacagctgca
     2581 tcggcaattg aatggtgatt gattagcaaa ctctctagct cagctggagg gacctgaaag
     2641 cctttgaact tgatgacttc tttaagtcga tcaacaatga aaatctcatc atcttcatca
     2701 acatatccaa tgtctcctgt gtgaagccaa ccttcttcgt ctattgttgc tgaagtggct
     2761 tctggatcgt tcaagtactc tttcatgatc tgttgaccgc ggatacaaat ctcaccaggt
     2821 tggttgtatc caagagagag acgtgtctca aggtgaacca ctttaagctc tgcgtttcgg
     2881 accacagtcc cacatgaacc tgattttgtc ggtatcggtt ctttagcgaa cccaaggctc
     2941 attgacaaca ctggtcctgc ctccgtcata ccatatccct ggccaaggat ggcttgaggg
     3001 agacggcgac gaagactatc ttgaagttct ttacctaaag gagctgcacc ggaaagaacg
     3061 aatctaacgg aggagagatc ataagaatta accgtcgggt ttttagctag agcgatcact
     3121 agcggcggta caagcgccgc tattgtcact ctatgtcttt gaataagatc caataacgca
     3181 ccgatctcaa acttatgcat taaaagaacc gtggcgccgg atcggagtga attgaggagg
     3241 acgctattga gagagtagat atggaaaagt ggtaaaacgc agagtatgac gtcgtttgat
     3301 ttgaggtaaa gatttgggtt atctccatct acttgttgtg caacgcttgt gattaagctc
     3361 ttgtgtgtca aaacaactcc ttttggtaac cctgttgttc ccgatgagaa tggtaacgcc
     3421 gccgcatcgt caccgccgat atcgacggtt tcttgaaatg ggtttgtttc gtcgtcggtg
     3481 atgagtgtcg agaatggtag acaattctcc ggtgttggtt catcggtagt gatcagcgtg
     3541 aggttttctc cgaggttttt aagtttatcg acgtattgag agtgagtgat tatgagtttg
     3601 gctccggaag atttaagctg tttataaagc tcttgagaag tgtagaaagg gtttgcggtg
     3661 gttgagacgg ctccgatcat ggaagcaccc atgaaggaga aaacgaactc ggctgagttt
     3721 tggagaagga tcatgatgac gtcacctttt ctgataccta acttgtataa cccggaagcg
     3781 actctccgac agattaggtg tgtttcgccg taggtgtagc ttttcccggt ggagccaacg
     3841 atgagacaag gcttgtcgga aacagatgag agtttttcga agcagtaagt gtggagaggg
     3901 aggtggttag ggatgtcaat gtccggaagc tttgaccgga aaatgcgagg cgtaggagga
     3961 gaatgaggaa gcacatcatc ggagacgacg gatgtatcgg ttggtttgtg aatctgaggt
     4021 tcgtgtagag ctgcagtgat catactagtt ctagaaaact tagattagat tgctatgctt
     4081 tctttctaat gagcaagaag taaaaaaagt tgtaatagaa caagaaaaat gaaactgaaa
     4141 cttgagaaat tgaagaccgt ttattaactt aaatatcaat gggaggtcat cgaaagagaa
     4201 aaaaatcaaa aaaaaaaatt ttcaagaaaa agaaacgtga taaaaatttt tattgccttt
     4261 ttcgacgaag aaaaagaaac gaggcggtct cttttttctt ttccaaacct ttagtacggg
     4321 taattaacga caccctagag gaagaaagag gggaaattta gtatgctgtg cttgggtgtt
     4381 ttgaagtggt acggcgatgc gcggagtccg agaaaatctg gaagagtaaa aaaggagtag
     4441 aaacattttg aagctatggg ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     4501 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     4561 ccgcgcttaa tgcgccgcta cagggcgcgt cgcgccattc gccattcagg ctgcgcaact
     4621 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaggggggat
     4681 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     4741 cgacggccag tgaattgtaa tacgactcac tatagggcga attggagctc gaagctcgga
     4801 tctacgtatg gtcatttctt cttcagattc cctcatggag aaagtgcggc agatgtatat
     4861 gacagagtcg ccagtttcca agagacttta ttcaggcact tccatgatag gcaagagaga
     4921 agacccagag atgttgttgt cctagttaca catggtattt attccagagt attcctgatg
     4981 aaatggttta gatggacata cgaagagttt gaatcgttta ccaatgttcc taacgggagc
     5041 gtaatggtga tggaactgga cgaatccatc aatagatacg tcctgaggac cgtgctaccc
     5101 aaatggactg attgtgaggg agacctaact acatagtgtt taaagattac ggatatttaa
     5161 cttacttaga ataatgccat ttttttgagt tataataatc ctacgttagt gtgagcggga
     5221 tttaaactgt gaggacctta atacattcag acacttctgc ggtatcaccc tacttattcc
     5281 cttcgagatt atatctagga acccatcagg ttggtggaag attacccgtt ctaagacttt
     5341 tcagcttcct ctattgatgt tacacctgga cacccctttt ctggcatcca gtttttaatc
     5401 ttcagtggca tgtgagattc tccgaaatta attaaagcaa tcacacaatt ctctcggata
     5461 ccacctcggt tgaaactgac aggtggtttg ttacgcatgc taatgcaaag gagcctatat
     5521 acctttggct cggctgctgt aacagggaat ataaagggca gcataattta ggagtttagt
     5581 gaacttgcaa catttactat tttcccttct tacgtaaata tttttctttt taattctaaa
     5641 tcaatctttt tcaatttttt gtttgtattc ttttcttgct taaatctata actacaaaaa
     5701 acacatacta gtatggtgat ggctggtgct tcttctttgg atgagatcag acaggctcag
     5761 agagctgatg gacctgcagg catcttggct attggcactg ctaaccctga gaaccatgtg
     5821 cttcaggcgg agtatcctga ctactacttc cgcatcacca acagtgaaca catgaccgac
     5881 ctcaaggaga agttcaagcg catgtgcgac aagtcgacaa ttcggaaacg tcacatgcat
     5941 ctgacggagg aattcctcaa ggaaaaccca cacatgtgtg cttacatggc tccttctctg
     6001 gacaccagac aggacatcgt ggtggtcgaa gtccctaagc taggcaaaga agcggcagtg
     6061 aaggccatca aggagtgggg ccagcccaag tcaaagatca ctcatgtcgt cttctgcact
     6121 acctccggcg tcgacatgcc tggtgctgac taccagctca ccaagcttct tggtctccgt
     6181 ccttccgtca agcgtctcat gatgtaccag caaggttgct tcgccggcgg tactgtcctc
     6241 cgtatcgcta aggatctcgc cgagaacaat cgtggagcac gtgtcctcgt tgtctgctct
     6301 gagatcacag ccgttacctt ccgtggtccc tctgacaccc accttgactc cctcgtcggt
     6361 caggctcttt tcagtgatgg cgccgccgca ctcattgtgg ggtcggaccc tgacacatct
     6421 gtcggagaga aacccatctt tgagatggtg tctgccgctc agaccatcct tccagactct
     6481 gatggtgcca tagacggaca tttgagggaa gttggtctca ccttccatct cctcaaggat
     6541 gttcccggcc tcatctccaa gaacattgtg aagagtctag acgaagcgtt taaacctttg
     6601 gggataagtg actggaactc cctcttctgg atagcccacc ctggaggtcc agcgatccta
     6661 gaccaggtgg agataaagct aggactaaag gaagagaaga tgagggcgac acgtcacgtg
     6721 ttgagcgagt atggaaacat gtcgagcgcg tgcgttctct tcatactaga cgagatgagg
     6781 aggaagtcag ctaaggatgg tgtggccacg acaggagaag ggttggagtg gggtgtcttg
     6841 tttggtttcg gaccaggtct cactgttgag acagtcgtct tgcacagcgt tcctctctaa
     6901 ctcgaggcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata
     6961 agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt
     7021 aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac
     7081 cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt cacccaattg
     7141 tagatatgct aactccagca atgagttgat gaatctcggt gtgtatttta tgtcctcaga
     7201 ggacaacacc tgttgtaatc gttcttccac gagctcttcg agtttatcat tatcaatact
     7261 cgccatttca aagaatacgt aaataattaa tagtagtgat tttcctaact ttatttagtc
     7321 aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta
     7381 cacagaatat ataacaccga aggtgcctgg gtgaacaggt ttattcctgg catccactaa
     7441 atataatgga gcccgctttt taagctggca tccagaaaaa aaaagaatcc cagcaccaaa
     7501 atattgtttt cttcaccaac catcagttca taggtccatt ctcttagcgc aactacagag
     7561 aacaggggca caaacaggca aaaaacgggc acaacctcaa tggagtgatg caacctgcct
     7621 ggagtaaatg atgacacaag gcaattgacc cacgcatgta tctatctcat tttcttacac
     7681 cttctattac cttctgctct ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag
     7741 ttccctgaaa ttattcccct acttgactaa taagtatata aagacggtag gtattgattg
     7801 taattctgta aatctatttc ttaaacttct taaattctac ttttatagtt agtctttttt
     7861 ttagttttaa aacaccagaa cttagtttcg acggattcta gaactagtat gtcttcatcc
     7921 aacgcctgcg cctctccgtc accgttcccc gccgtcacga agcttcatgt agactccgtc
     7981 acgtttgtac cgtccgtcaa gtcaccggcc tcctccaatc cattattcct cggcggcgcc
     8041 ggtgtccgag gccttgatat ccaaggtaaa ttcgtgatct tcaccgtcat tggagtatac
     8101 ctagagggta acgccgttcc ttctctatct gtcaagtgga agggaaaaac tacggaggag
     8161 ctaacagaat ctatcccgtt cttccgtgaa atagtcaccg gtgcgtttga gaagtttatc
     8221 aaggtgacaa tgaaactgcc gttaacggga caacaatatt cggagaaagt gacggagaat
     8281 tgtgtggcta tatggaaaca attagggctt tatacggact gtgaagctaa agctgtggag
     8341 aagttcttgg agatcttcaa ggaagaaaca ttccctcccg gttcatcgat cctcttcgct
     8401 ctctccccta ccggctctct tacggttgcg ttttcgaaag atgatagtat ccctgaaacc
     8461 gggatcgctg tgatcgagaa caaattgttg gcggaggcgg ttctggaatc tatcatcggg
     8521 aagaacggtg tgtcacctgg cactaggtta agtgttgcag aaagattatc tcagctaatg
     8581 atgaagaaca aggacgaaaa ggaagttagt gatcactctg ttgaggaaaa actagccaaa
     8641 gagaactgag tcgacctcga gtcatgtaat tagttatgtc acgcttacat tcacgccctc
     8701 cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta
     8761 tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt
     8821 tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg
     8881 ttttgggacg ctcgaaggct ttaatttgcg gccgtcgagg gggggcccgg taccagcttt
     8941 tgttcccttt agtgagggtt aattccgagc ttggcgtaat catggtcata gctgtttcct
     9001 gtgtgaaatt gttatccgct cacaattcca cacaacatag gagccggaag cataaagtgt
     9061 aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc
     9121 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9181 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9241 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9301 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9361 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcg gcccccctga cgagcatcac
     9421 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9481 ttcccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9541 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     9601 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9661 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9721 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9781 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     9841 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9901 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9961 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10021 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10081 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10141 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10201 tccatagttg cctgactgcc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10261 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10321 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10381 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10441 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10501 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgaaaa
    10561 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10621 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10681 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10741 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10801 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10861 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10921 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10981 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11041 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11101 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    11161 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    11221 gatgacggtg aaaacctctg acacatgcag ctatgccaga gtccagattg cagagactag
    11281 ctaatttgaa aataggaact ccgcagcagc tcagacgcac ctccataata ggtaccattg
    11341 ggcccaagac aaatagctgc gaggccatta ctgctctgag aaaagctggt ttgaacatca
    11401 ttcgattgaa cttttcccat ggctcctacg aattccatca atcagtaatc gaaaatgctg
    11461 tgaaatcgga acagcaattc cctggcaggc cgctcgccat tgccctggat accaagggtc
    11521 ccgagatcag aacaggtcgc acgttaaatg accaagatct ttatatcccc gtagaccacc
    11581 aaatgatctt taccactgac gcaagttttg caaacacctc caatgataaa atcatgtata
    11641 tagactatgc taacctgaca aaagttatcg ttccggggag atttatatac gtggacgacg
    11701 ggattctctc ttttaaagtg ctccaaatca ttgacgaatc taatttaagg gtgcaagcgg
    11761 taaactcggg ttatatcgca tctcataaag gtgttaatct gcctaatacc gacgttgatt
    11821 tgcccccctt gtccgccaaa gacatgaagg acttgcaatt cggagtccgc aatggcattc
    11881 acatcgtatt tgcctctttc ataagaactt cagaagatgt gttgtctatc agaaaagcgt
    11941 tgggttctga agggcaagat atctcccgga gacggtcaca gcttgtctgt aagcggatgc
    12001 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    12061 taactatgcg gcatcagagc agattgtact gagagtgcac catatcgact acgtcgtaag
    12121 gccgtttctg acagagtaaa attcttgagg gaactttcac cattatggga aatggttcaa
    12181 gaaggtattg acttaaactc catcaaatgg tcaggtcatt gagtgttttt tatttgttgt
    12241 attttttttt ttttagagaa aatcctccaa tatcaaatta ggaatcgtag tttcatgatt
    12301 ttctgttaca cctaactttt tgtgtggtgc cctcctcctt gtcaatatta atgttaaagt
    12361 gcaattcttt ttccttatca cgttgagcca ttagtatcaa tttgcttacc tgtattcctt
    12421 tactatcctc ctttttctcc ttcttgataa atgtatgtag attgcgtata tagtttcgtc
    12481 taccctatga acatattcca ttttgtaatt tcgtgtcgtt tctattatga atttcattta
    12541 taaagtttat gtacaaatat cataaaaaaa gagaat
//
