pUCPMA-M24 vector (V002354)

MSDS

Basic Vector Information

Vector Name:: pUCPMA-M24

Antibiotic Resistance:: Ampicillin

Length:: 4453 bp

Type:: Transient gene expression vector

Replication origin:: ori

Source/Author:: Maiti IB, Dey N.

pUCPMA-M24 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUCPMA-M24 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45388        4453 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transient gene expression vector pUCPMA-M24, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Maiti IB, Dey N.
  TITLE     Further characterization and expression analysis of Mirabilis mosaic
            caulimovirus (MMV) full-length transcript promoter with single and 
            double enhancer domains in transgenic plants
  JOURNAL   Transgenics 3, 61-70 (1999)
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Dey N, Maiti IB.
  TITLE     Structure and promoter/leader deletion analysis of mirabilis mosaic 
            virus (MMV) full-length transcript promoter in transgenic plants
  JOURNAL   Plant Mol. Biol. 40 (5), 771-782 (1999)
  PUBMED    10487212
REFERENCE   3  (bases 1 to 4453)
  AUTHORS   Maiti IB, Dey N, Shepherd RJ.
  TITLE     Use of the full-length transcript (FLt) from Mirabilis mosaic 
            caulimovirus to express chimeric genes in plants
  JOURNAL   Patent: US US6420547B1-B 16-JUL-2002;
REFERENCE   4  (bases 1 to 4453)
  AUTHORS   Maiti IB, Dey N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2015) KTRDC, Plant Genetic Engineering Research 
            and Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA
REFERENCE   5  (bases 1 to 4453)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 4453)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenics
            3, 61-70 (1999)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "1999"; volume: "40"; issue: "5"; pages: "771-782"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Patent: US 
            US6420547B1-B 16-JUL-2002;"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2015) KTRDC, Plant Genetic Engineering Research and 
            Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA"
COMMENT     SGRef: number: 5; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(6..647)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    complement(647..692)
                     /note="multiple cloning site; XbaI-SacI-XhoI-BamHI-HindIII"
     regulatory      complement(692..1320)
                     /note="M24 promoter; derived from Mirabilis mosaic virus
                     (MMV)"
                     /regulatory_class="promoter"
     misc_feature    complement(1321..1326)
                     /label=EcoRI restriction enzyme site
                     /note="EcoRI restriction enzyme site"
     primer_bind     complement(1327..1343)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1556..2011
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2293..2397
                     /label=AmpR promoter
     CDS             2398..3255
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      3259..3417
                     /label=ampR terminator
                     /note="ampR terminator"
                     /regulatory_class="polyA_signal_sequence"
     rep_origin      3429..4017
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4305..4326
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4341..4371
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4379..4395
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4403..4419
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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