LOCUS       40924_45388        4453 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transient gene expression vector pUCPMA-M24, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Maiti IB, Dey N.
  TITLE     Further characterization and expression analysis of Mirabilis mosaic
            caulimovirus (MMV) full-length transcript promoter with single and 
            double enhancer domains in transgenic plants
  JOURNAL   Transgenics 3, 61-70 (1999)
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Dey N, Maiti IB.
  TITLE     Structure and promoter/leader deletion analysis of mirabilis mosaic 
            virus (MMV) full-length transcript promoter in transgenic plants
  JOURNAL   Plant Mol. Biol. 40 (5), 771-782 (1999)
  PUBMED    10487212
REFERENCE   3  (bases 1 to 4453)
  AUTHORS   Maiti IB, Dey N, Shepherd RJ.
  TITLE     Use of the full-length transcript (FLt) from Mirabilis mosaic 
            caulimovirus to express chimeric genes in plants
  JOURNAL   Patent: US US6420547B1-B 16-JUL-2002;
REFERENCE   4  (bases 1 to 4453)
  AUTHORS   Maiti IB, Dey N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2015) KTRDC, Plant Genetic Engineering Research 
            and Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA
REFERENCE   5  (bases 1 to 4453)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 4453)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenics
            3, 61-70 (1999)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "1999"; volume: "40"; issue: "5"; pages: "771-782"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Patent: US 
            US6420547B1-B 16-JUL-2002;"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2015) KTRDC, Plant Genetic Engineering Research and 
            Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA"
COMMENT     SGRef: number: 5; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(6..647)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    complement(647..692)
                     /note="multiple cloning site; XbaI-SacI-XhoI-BamHI-HindIII"
     regulatory      complement(692..1320)
                     /note="M24 promoter; derived from Mirabilis mosaic virus
                     (MMV)"
                     /regulatory_class="promoter"
     misc_feature    complement(1321..1326)
                     /label=EcoRI restriction enzyme site
                     /note="EcoRI restriction enzyme site"
     primer_bind     complement(1327..1343)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1556..2011
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2293..2397
                     /label=AmpR promoter
     CDS             2398..3255
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      3259..3417
                     /label=ampR terminator
                     /note="ampR terminator"
                     /regulatory_class="polyA_signal_sequence"
     rep_origin      3429..4017
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4305..4326
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4341..4371
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4379..4395
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4403..4419
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgattgat gcatgttgtc aatcaattgg caagtcataa aatgcattaa aaaatatttt
       61 catactcaac tacaaatcca tgagtataac tataattata aagcaatgat tagaatctga
      121 caaggattct ggaaaattac ataaaggaaa gttcataaat gtctaaaaca caagaggaca
      181 tacttgtatt cagtaacatt tgcagctttt ctaggtctga aaatatattt gttgcctagt
      241 gaataagcat aatggtacaa ctacaagtgt tttactcctc atattaactt cggtcattag
      301 aggccacgat ttgacacatt tttactcaaa acaaaatgtt tgcatatctc ttataatttc
      361 aaattcaaca cacaacaaat aagagaaaaa acaaataata ttaatttgag aatgaacaaa
      421 aggaccatat cattcattaa ctcttctcca tccatttcca tttcacagtt cgatagcgaa
      481 aaccgaataa aaaacacagt aaattacaag cacaacaaat ggtacaagaa aaacagtttt
      541 cccaatgcca taatactcaa actcagtagg attctggtgt gtgcgcaatg aaactgatgc
      601 attgaacttg acgaacgttg tcgaaaccga tgatacgaac gaaagctcta gaggatcact
      661 tcgagctcct gcagctcgag gatccaagct tagatgagag atttcgattc cgattttgat
      721 ttcgattccg attttgattt cgattgatct cttccttctg atttgtgttc cttatataag
      781 gaaattcttg tgggattaga cgtcatggct tacgtcattt ccttcgtcct gttgctcact
      841 gattgagctg tgagtggagg gaccactgga agatgcttca ctaattttct tagtggaggg
      901 accggcttct catgcttcac acaagtggct gtcgggcatc atctttttta gcttttgaca
      961 aagcaatgtt ttagtggtgg ctcccactct tatcttcaac attattatct tatcttcaaa
     1021 ggacgataag atgttgatgt ctgtggacga agttgggatt agacgtcatg gcttacgtca
     1081 tttccttcgt cctgttgctc actgattgag ctgtgagtgg agggaccact ggaagatgct
     1141 tcactaattt tcttagtgga gggaccggct tctcatgctt cacacaagtg gctgtcgggc
     1201 atcatctttt ttagcttttg acaaagcaat gttttagtgg gggctcccac tcttatcttc
     1261 aacattatta tcttatcttc aaaggacgat aagatgttga tgtctgtgga cgaagttgac
     1321 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     1381 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     1441 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     1501 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     1561 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     1621 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1681 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     1741 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     1801 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1861 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     1921 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     1981 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     2041 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2101 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2161 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     2221 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2281 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2341 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2401 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2461 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2521 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2581 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2641 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2701 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2761 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2821 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2881 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2941 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3001 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3061 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3121 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3181 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3241 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3301 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3361 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3421 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3481 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3541 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3601 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3661 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3721 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3781 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3841 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3901 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3961 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4021 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4081 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4141 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4201 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4261 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     4321 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     4381 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctagct
     4441 tgcatgcctg cag
//
