pUCP24T vector (V002363)

Basic Vector Information

      • Vector Name:
      • pUCP24T
      • Antibiotic Resistance:
      • Gentamicin
      • Length:
      • 4381 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Lu Y, Zeng J, Wu B, E S, Wang L, Cai R, Zhang N, Li Y, Huang X, Huang B, Chen C.

pUCP24T vector Vector Map

pUCP24T4381 bp600120018002400300036004200pRO1600 oriVpRO1600 RepM13 fwdoriTM13 revlac operatorlac promoterCAP binding siteoriGmRPc promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUCP24T vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45358        4381 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUCP24T, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Lu Y, Zeng J, Wu B, E S, Wang L, Cai R, Zhang N, Li Y, Huang X, 
            Huang B, Chen C.
  TITLE     Quorum Sensing N-acyl Homoserine Lactones-SdiA Suppresses 
            Escherichia coli-Pseudomonas aeruginosa Conjugation through 
            Inhibiting traI Expression
  JOURNAL   Front Cell Infect Microbiol 7, 7 (2017)
  PUBMED    28164039
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Chen S, E S, Xiao Q, Zeng J, Lu Y, Ma X, He Y, Huang B, Chen C, 
            Zhang N, Cai R, Yuan H, Qu P, Lin D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2017) Department of Laboratory Medicine, The First
            Affiliated Hospital of Sun Yat-sen University, Zhongshan road, 
            Yuexiu District, Guangzhou, Guangdong 510000, China
REFERENCE   3  (bases 1 to 4381)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4381)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol 7, 7 (2017)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-2017) Department of Laboratory Medicine, The First 
            Affiliated Hospital of Sun Yat-sen University, Zhongshan road, 
            Yuexiu District, Guangzhou, Guangdong 510000, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(46..397)
                     /direction=LEFT
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     regulatory      398..401
                     /gene="rep"
                     /regulatory_class="ribosome_binding_site"
     CDS             411..1241
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     primer_bind     1405..1421
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     oriT            1601..1710
                     /label=oriT
                     /note="incP origin of transfer"
     primer_bind     complement(1836..1852)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1860..1876)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1884..1914)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1929..1950)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2238..2826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3630..4160)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     regulatory      complement(4169..4174)
                     /gene="aacC1"
                     /regulatory_class="ribosome_binding_site"
     promoter        complement(4349..4377)
                     /label=Pc promoter
                     /note="class 1 integron promoter"

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