pUCP24T vector (V002363)

LOCUS       40924_45358        4381 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUCP24T, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Lu Y, Zeng J, Wu B, E S, Wang L, Cai R, Zhang N, Li Y, Huang X, 
            Huang B, Chen C.
  TITLE     Quorum Sensing N-acyl Homoserine Lactones-SdiA Suppresses 
            Escherichia coli-Pseudomonas aeruginosa Conjugation through 
            Inhibiting traI Expression
  JOURNAL   Front Cell Infect Microbiol 7, 7 (2017)
  PUBMED    28164039
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Chen S, E S, Xiao Q, Zeng J, Lu Y, Ma X, He Y, Huang B, Chen C, 
            Zhang N, Cai R, Yuan H, Qu P, Lin D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2017) Department of Laboratory Medicine, The First
            Affiliated Hospital of Sun Yat-sen University, Zhongshan road, 
            Yuexiu District, Guangzhou, Guangdong 510000, China
REFERENCE   3  (bases 1 to 4381)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4381)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol 7, 7 (2017)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-2017) Department of Laboratory Medicine, The First 
            Affiliated Hospital of Sun Yat-sen University, Zhongshan road, 
            Yuexiu District, Guangzhou, Guangdong 510000, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(46..397)
                     /direction=LEFT
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     regulatory      398..401
                     /gene="rep"
                     /regulatory_class="ribosome_binding_site"
     CDS             411..1241
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     primer_bind     1405..1421
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     oriT            1601..1710
                     /label=oriT
                     /note="incP origin of transfer"
     primer_bind     complement(1836..1852)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1860..1876)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1884..1914)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1929..1950)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2238..2826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3630..4160)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     regulatory      complement(4169..4174)
                     /gene="aacC1"
                     /regulatory_class="ribosome_binding_site"
     promoter        complement(4349..4377)
                     /label=Pc promoter
                     /note="class 1 integron promoter"