Basic Vector Information
pUCP24T vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUCP24T vector Sequence
LOCUS 40924_45358 4381 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pUCP24T, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4381) AUTHORS Lu Y, Zeng J, Wu B, E S, Wang L, Cai R, Zhang N, Li Y, Huang X, Huang B, Chen C. TITLE Quorum Sensing N-acyl Homoserine Lactones-SdiA Suppresses Escherichia coli-Pseudomonas aeruginosa Conjugation through Inhibiting traI Expression JOURNAL Front Cell Infect Microbiol 7, 7 (2017) PUBMED 28164039 REFERENCE 2 (bases 1 to 4381) AUTHORS Chen S, E S, Xiao Q, Zeng J, Lu Y, Ma X, He Y, Huang B, Chen C, Zhang N, Cai R, Yuan H, Qu P, Lin D. TITLE Direct Submission JOURNAL Submitted (10-MAY-2017) Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Zhongshan road, Yuexiu District, Guangzhou, Guangdong 510000, China REFERENCE 3 (bases 1 to 4381) TITLE Direct Submission REFERENCE 4 (bases 1 to 4381) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell Infect Microbiol 7, 7 (2017)" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (10-MAY-2017) Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Zhongshan road, Yuexiu District, Guangzhou, Guangdong 510000, China" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..4381 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(46..397) /direction=LEFT /label=pRO1600 oriV /note="broad-host-range origin of replication from Pseudomonas aeruginosa plasmid pRO1600; requires the pRO1600 Rep protein for replication (West et al., 1994)" regulatory 398..401 /gene="rep" /regulatory_class="ribosome_binding_site" CDS 411..1241 /label=pRO1600 Rep /note="replication protein for the broad-host-range plasmid pRO1600 from Pseudomonas aeruginosa" primer_bind 1405..1421 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" oriT 1601..1710 /label=oriT /note="incP origin of transfer" primer_bind complement(1836..1852) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1860..1876) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1884..1914) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1929..1950) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(2238..2826) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3630..4160) /label=GmR /note="gentamycin acetyltransferase" regulatory complement(4169..4174) /gene="aacC1" /regulatory_class="ribosome_binding_site" promoter complement(4349..4377) /label=Pc promoter /note="class 1 integron promoter"
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