LOCUS       40924_45358        4381 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUCP24T, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Lu Y, Zeng J, Wu B, E S, Wang L, Cai R, Zhang N, Li Y, Huang X, 
            Huang B, Chen C.
  TITLE     Quorum Sensing N-acyl Homoserine Lactones-SdiA Suppresses 
            Escherichia coli-Pseudomonas aeruginosa Conjugation through 
            Inhibiting traI Expression
  JOURNAL   Front Cell Infect Microbiol 7, 7 (2017)
  PUBMED    28164039
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Chen S, E S, Xiao Q, Zeng J, Lu Y, Ma X, He Y, Huang B, Chen C, 
            Zhang N, Cai R, Yuan H, Qu P, Lin D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2017) Department of Laboratory Medicine, The First
            Affiliated Hospital of Sun Yat-sen University, Zhongshan road, 
            Yuexiu District, Guangzhou, Guangdong 510000, China
REFERENCE   3  (bases 1 to 4381)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4381)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol 7, 7 (2017)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-2017) Department of Laboratory Medicine, The First 
            Affiliated Hospital of Sun Yat-sen University, Zhongshan road, 
            Yuexiu District, Guangzhou, Guangdong 510000, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(46..397)
                     /direction=LEFT
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     regulatory      398..401
                     /gene="rep"
                     /regulatory_class="ribosome_binding_site"
     CDS             411..1241
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     primer_bind     1405..1421
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     oriT            1601..1710
                     /label=oriT
                     /note="incP origin of transfer"
     primer_bind     complement(1836..1852)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1860..1876)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1884..1914)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1929..1950)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2238..2826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3630..4160)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     regulatory      complement(4169..4174)
                     /gene="aacC1"
                     /regulatory_class="ribosome_binding_site"
     promoter        complement(4349..4377)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 cctctcaggc gccgctggtg ccgctggttg gacgccaagg gtgaatccgc ctcgataccc
       61 tgattactcg cttcctgcgc cctctcaggc ggcgataggg gactggtaaa acggggattg
      121 cccagacgcc tcccccgccc cttcaggggc acaaatgcgg ccccaacggg gccacgtagt
      181 ggtgcgtttt ttgcgtttcc acccttttct tccttttccc ttttaaacct tttaggacgt
      241 ctacaggcca cgtaatccgt ggcctgtaga gtttaaaaag ggacggattt gttgccatta
      301 agggacggat ttgttgttaa gaagggacgg atttgttgtt gtaaagggac ggatttgttg
      361 tattgtggga cgcagataca gtgtcccctt atacacaagg aatgtcgaac gtggcctcac
      421 ccccaatggt ttacaaaagc aatgccctgg tcgaggccgc gtatcgcctc agtgttcagg
      481 aacagcggat cgttctggcc tgtattagcc aggtgaagag gagcgagcct gtcaccgatg
      541 aagtgatgta ttcagtgacg gcggaggaca tagcgacgat ggcgggtgtc cctatcgaat
      601 cttcctacaa ccagctcaaa gaagcggccc tgcgcctgaa acggcgggaa gtccggttaa
      661 cccaagagcc caatggcaag gggaaaagac cgagtgtgat gattaccggc tgggtgcaaa
      721 caatcatcta ccgggagggt gagggccgtg tagaactcag gttcaccaaa gacatgctgc
      781 cgtacctgac ggaactcacc aaacagttca ccaaatacgc cttggctgac gtggccaaga
      841 tggacagcac ccacgcgatc aggctttacg agctgctcat gcaatgggac agcatcggcc
      901 agcgcgaaat agaaattgac cagctgcgaa agtggtttca actggaaggc cggtatccct
      961 cgatcaagga cttcaagttg cgagtgcttg atccagccgt gacgcagatc aacgagcaca
     1021 gcccgctaca ggtggagtgg gcgcagcgaa agaccgggcg caaggtcaca catctgttgt
     1081 tcagttttgg accgaagaag cccgccaagg cggtgggtaa ggccccagcg aagcgcaagg
     1141 ccgggaagat ttcagatgct gagatcgcga aacaggctcg ccctggtgag acatgggaag
     1201 cggcccgcgc tcgactaacc cagatgccgc tggatctggc ctagaggccg tggccaccac
     1261 ggcccggcct gcctttcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     1321 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     1381 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gggtagatat
     1441 tccacaaaac agcagggaag cagcgctttt ccgctgcata accctgcttc ggggtcatta
     1501 tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg
     1561 ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag
     1621 gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca
     1681 acgggaatcc tgctctgcga ggctggccgg ctaccgccgg cgtaacagat gagggcaagc
     1741 ggatggctga tgaaaccaag ccaaccagga agggcagccc acctatcaag gtgtactgcc
     1801 ttcccccggg taccgagctc gaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1861 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1921 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1981 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2041 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2101 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2161 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2221 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2281 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2341 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2401 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2461 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2521 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2581 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2641 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2701 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2761 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2821 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2881 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2941 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3001 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3061 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3121 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3181 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3241 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3301 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3361 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3421 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3481 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3541 actggtgagt cgaattggcc gcggcgttgt gacaatttac cgaacaactc cgcggccggg
     3601 aagccgatct cggcttgaac gaattgttag gtggcggtac ttgggtcgat atcaaagtgc
     3661 atcacttctt cccgtatgcc caactttgta tagagagcca ctgcgggatc gtcaccgtaa
     3721 tctgcttgca cgtagatcac ataagcacca agcgcgttgg cctcatgctt gaggagattg
     3781 atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag atcatagata
     3841 tagatctcac tacgcggctg ctcaaacctg ggcagaacgt aagccgcgag agcgccaaca
     3901 accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct tactacggag caagttcccg
     3961 aggtaatcgg agtccggctg atgttgggag taggtggcta cgtctccgaa ctcacgaccg
     4021 aaaagatcaa gagcagcccg catggatttg acttggtcag ggccgagcct acatgtgcga
     4081 atgatgccca tacttgagcc acctaacttt gttttagggc gactgccctg ctgcgtaaca
     4141 tcgttgctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
     4201 acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc
     4261 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     4321 gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt atgtcaattc
     4381 g
//
