Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V002382 | pUCDMIG | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
pUCDMIG vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUCDMIG vector Sequence
LOCUS 40924_45253 4279 bp DNA circular SYN 18-DEC-2018 DEFINITION Baculovirus expression vector pUCDMIG, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4279) AUTHORS Yao L, Sun J. TITLE Construction of a baculovirus-silkworm multigene expression system and its application on producing virus-like particles JOURNAL Unpublished REFERENCE 2 (bases 1 to 4279) AUTHORS Yao L, Sun J. TITLE Direct Submission JOURNAL Submitted (18-AUG-2011) Hen Nan Provincial Key Laboratory of Funiu Mountain Insect Biology, Nan Yang Normal University, 1638 Wolong Road, Nan Yang, He Nan 473061, China REFERENCE 3 (bases 1 to 4279) TITLE Direct Submission REFERENCE 4 (bases 1 to 4279) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (18-AUG-2011) Hen Nan Provincial Key Laboratory of Funiu Mountain Insect Biology, Nan Yang Normal University, 1638 Wolong Road, Nan Yang, He Nan 473061, China" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4279 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(6..394) /direction=LEFT /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" protein_bind complement(415..448) /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." polyA_signal complement(638..686) /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" promoter complement(822..931) /label=p10 promoter /note="baculovirus promoter for expression in insect cells" promoter 976..1067 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" 5'UTR 1175..1754 /note="5'-UTR internal ribosome entry site (IRES) sequence of Rhopalosiphum padi virus" CDS 1764..2480 /label=EGFP /note="enhanced GFP" polyA_signal 2626..2760 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" terminator 2846..2893 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" promoter 3131..3233 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 3234..3890 /label=CmR /note="chloramphenicol acetyltransferase"