pUCDMIG vector (V002382)

Price Information

Cat No. Plasmid Name Availability Add to cart
V002382 pUCDMIG In stock (lyophilized plasmid)

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Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

      • Vector Name:
      • pUCDMIG
      • Antibiotic Resistance:
      • Chloramphenicol
      • Length:
      • 4279 bp
      • Type:
      • Baculovirus expression vector
      • Source/Author:
      • Yao L, Sun J.
      • Growth Strain(s):
      • GT115
      • Growth Temperature:
      • 37℃

pUCDMIG vector Vector Map

pUCDMIG4279 bp600120018002400300036004200R6K gamma oriloxPHSV TK poly(A) signalp10 promoterpolyhedrin promoter5'UTREGFPSV40 poly(A) signalT7 terminatorcat promoterCmR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUCDMIG vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45253        4279 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression vector pUCDMIG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Yao L, Sun J.
  TITLE     Construction of a baculovirus-silkworm multigene expression system 
            and its application on producing virus-like particles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Yao L, Sun J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Hen Nan Provincial Key Laboratory of Funiu 
            Mountain Insect Biology, Nan Yang Normal University, 1638 Wolong 
            Road, Nan Yang, He Nan 473061, China
REFERENCE   3  (bases 1 to 4279)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4279)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Hen Nan Provincial Key Laboratory of Funiu Mountain 
            Insect Biology, Nan Yang Normal University, 1638 Wolong Road, Nan 
            Yang, He Nan 473061, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..394)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(415..448)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(638..686)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(822..931)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        976..1067
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     5'UTR           1175..1754
                     /note="5'-UTR internal ribosome entry site (IRES) sequence
                     of Rhopalosiphum padi virus"
     CDS             1764..2480
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    2626..2760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2846..2893
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3131..3233
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3234..3890
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"