LOCUS       40924_45253        4279 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression vector pUCDMIG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Yao L, Sun J.
  TITLE     Construction of a baculovirus-silkworm multigene expression system 
            and its application on producing virus-like particles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Yao L, Sun J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Hen Nan Provincial Key Laboratory of Funiu 
            Mountain Insect Biology, Nan Yang Normal University, 1638 Wolong 
            Road, Nan Yang, He Nan 473061, China
REFERENCE   3  (bases 1 to 4279)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4279)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Hen Nan Provincial Key Laboratory of Funiu Mountain 
            Insect Biology, Nan Yang Normal University, 1638 Wolong Road, Nan 
            Yang, He Nan 473061, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..394)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(415..448)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(638..686)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(822..931)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        976..1067
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     5'UTR           1175..1754
                     /note="5'-UTR internal ribosome entry site (IRES) sequence
                     of Rhopalosiphum padi virus"
     CDS             1764..2480
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    2626..2760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      2846..2893
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3131..3233
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3234..3890
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 aattctgtca gccgttaagt gttcctgtgt cactcaaaat tgctttgaga ggctctaagg
       61 gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaagcttt
      121 aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt
      181 tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga aacatgagag
      241 cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt tcgttaaaca
      301 tgagagctta gtacgttaaa catgagagct tagtacgtga aacatgagag cttagtacgt
      361 actatcaaca ggttgaactg ctgatcaaca gatcctctac gcggccgcgg taccataact
      421 tcgtatagca tacattatac gaagttatct ggtttaaacg tacccgtagt ggctatggca
      481 gggcttgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tgggggttgg
      541 ggtggggaaa aggaagaaac gcgggcgtat tggtcccaat ggggtctcgg tggggtatcg
      601 acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacacccgtg
      661 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt
      721 ccgtgtttca gttagcctcc cccatctccc ggtaccgcat gctatgcatc agctgctagc
      781 accatggctc gagatcccgg gtgatcaagt cttcgtcgag tgattgtaaa taaaatgtaa
      841 tttacagtat agtattttaa ttaatataca aatgatttga taataattct tatttaacta
      901 taatatattg tgttgggttg aattaaaggt ccgtatacta gtatcgattc gcgacctact
      961 ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca aataaataag
     1021 tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt ccggattatt
     1081 cataccgtcc caccatcggg cgcggatccc ggtccgaagc gcgcggaatt caaaggccta
     1141 cgtcgacgag ctcactagtc gcggccgctt tcgaagataa aagaacctat aatcccttcg
     1201 cacaccgcgt cacaccgcgc tatatgctgc tcattaggaa ttacggctcc ttttttgtgg
     1261 atacaatctc ttgtatacga tatacttatt gttaatttca ttgaccttta cgcaatcctg
     1321 cgtaaatgct ggtatagggt gtacttcgga tttccgagcc tatattggtt ttgaaaggac
     1381 ctttaagtcc ctactatact acattgtact agcgtaggcc acgtaggccc gtaagatatt
     1441 ataactattt tattatattt tattcacccc ccacattaat cccagttaaa gctttataac
     1501 tataagtaag ccgtgccgaa acgttaatcg gtcgctagtt gcgtaacaac tgttagttta
     1561 attttccaaa atttattttt cacaattttt agttaagatt ttagcttgcc ttaagcagtc
     1621 tttatatctt ctgtatatta ttttaaagtt tataggagca aagttcgctt tactcgcaat
     1681 agctatttta tttattttag gaatattatc acctcgtaat tatttaatta taacattagc
     1741 tttatctatt tatatctagt aacatggtga gcaagggcga ggagctgttc accggggtgg
     1801 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1861 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     1921 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     1981 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2041 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2101 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2161 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2221 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     2281 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     2341 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     2401 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     2461 gcatggacga gctgtacaag taactgcagt ctcgacaagc ttgtcgagaa gtactagagg
     2521 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     2581 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     2641 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     2701 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     2761 tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     2821 ggctgctgcc accgctgagc aataactatc ataaccccta ggggcctcta aacgggtctt
     2881 gaggggtttt ttgctgaaag gaggaactat atccggatct gaacaggagg gacagctgat
     2941 agaaacagaa gccactggag cacctcaaaa acaccatcat acactaaatc agtaagttgg
     3001 cagcatcacc cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag
     3061 aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa
     3121 aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
     3181 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga
     3241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     3301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     3361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     3421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     3481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     3541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     3601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     3661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     3721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     3781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     3841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt
     3901 aaggcagtta ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc
     3961 ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg
     4021 ttaaatagcc gcttatgtct attgctggtt taccggttta ttgactaccg gaagcagtgt
     4081 gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag gctctccccg tggaggtaat
     4141 aattgacgat atgatcattt attctgcctc ccagctgaca ttcatccggg gtcagcaccg
     4201 tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg
     4261 cttgcggcag cgtgaagct
//
