pUCAG.TBX5 vector (V002384)

Basic Vector Information

      • Vector Name:
      • pUCAG.TBX5
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6630 bp
      • Type:
      • Shuttle vector
      • Source/Author:
      • van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ, Atsma DE.

pUCAG.TBX5 vector Vector Map

pUCAG.TBX56630 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600M13 revCMV.IE enhancerchicken beta-actin promoterchimeric intronTBX5beta-globin poly(A) signalM13 fwdAmpR promoterAmpRoriCAP binding sitelac promoterlac operator

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUCAG.TBX5 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45238        6630 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pUCAG.TBX5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der 
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ, 
            Atsma DE.
  TITLE     Fibroblasts from human postmyocardial infarction scars acquire 
            properties of cardiomyocytes after transduction with a recombinant 
            myocardin gene
  JOURNAL   FASEB J. 21 (12), 3369-3379 (2007)
  PUBMED    17579192
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 
            RC, Netherlands
REFERENCE   3  (bases 1 to 6630)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6630)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC, 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      68..448
                     /label=CMV.IE enhancer
                     /note="CMV.IE enhancer"
                     /regulatory_class="enhancer"
     enhancer        68..447
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        450..725
                     /label=chicken beta-actin promoter
     intron          726..1734
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1862..3415
                     /gene="TBX5"
                     /label=TBX5
                     /note="T-box transcription factor TBX5 from Homo sapiens. 
                     Accession#: Q99593"
     polyA_signal    3599..3654
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4031..4047)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4521..4625
                     /label=AmpR promoter
     CDS             4626..5483
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5657..6245
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6533..6554
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6569..6599
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6607..6623
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

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