LOCUS       40924_45238        6630 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pUCAG.TBX5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der 
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ, 
            Atsma DE.
  TITLE     Fibroblasts from human postmyocardial infarction scars acquire 
            properties of cardiomyocytes after transduction with a recombinant 
            myocardin gene
  JOURNAL   FASEB J. 21 (12), 3369-3379 (2007)
  PUBMED    17579192
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 
            RC, Netherlands
REFERENCE   3  (bases 1 to 6630)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6630)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC, 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      68..448
                     /label=CMV.IE enhancer
                     /note="CMV.IE enhancer"
                     /regulatory_class="enhancer"
     enhancer        68..447
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        450..725
                     /label=chicken beta-actin promoter
     intron          726..1734
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1862..3415
                     /gene="TBX5"
                     /label=TBX5
                     /note="T-box transcription factor TBX5 from Homo sapiens. 
                     Accession#: Q99593"
     polyA_signal    3599..3654
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4031..4047)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4521..4625
                     /label=AmpR promoter
     CDS             4626..5483
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5657..6245
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6533..6554
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6569..6599
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6607..6623
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcttgttta aacatttaaa tctcgagcca
       61 tggattcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      121 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      181 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      241 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      301 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      361 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      421 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      481 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      541 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
      601 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      661 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      721 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      781 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      841 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      901 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      961 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
     1021 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1081 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1141 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1201 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg
     1261 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1321 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc
     1381 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1441 cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc
     1501 cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1561 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag
     1621 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1681 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1741 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattatc gcatgcctgc
     1801 agagctctag agtcgacttg gagagcagaa ccttgcgcgg gcacagggcc ctgggcgcac
     1861 catggccgac gcagacgagg gctttggctt ggcgcacacg cctctggagc ctgacgcaaa
     1921 agacctgccc tgcgattcga aacccgagag cgcgctcggg gcccccagca agtccccgtc
     1981 gtccccgcag gccgccttca cccagcaggg catggaggga atcaaagtgt ttctccatga
     2041 aagagaactg tggctaaaat tccacgaagt gggcacggaa atgatcataa ccaaggctgg
     2101 aaggcggatg tttcccagtt acaaagtgaa ggtgacgggc cttaatccca aaacgaagta
     2161 cattcttctc atggacattg tacctgccga cgatcacaga tacaaattcg cagataataa
     2221 atggtctgtg acgggcaaag ctgagcccgc catgcctggc cgcctgtacg tgcacccaga
     2281 ctcccccgcc accggggcgc attggatgag gcagctcgtc tccttccaga aactcaagct
     2341 caccaacaac cacctggacc catttgggca tattattcta aattccatgc acaaatacca
     2401 gcctagatta cacatcgtga aagcggatga aaataatgga tttggctcaa aaaatacagc
     2461 gttctgcact cacgtctttc ctgagactgc gtttatagca gtgacttcct accagaacca
     2521 caagatcacg caattaaaga ttgagaataa tccctttgcc aagggatttc ggggcagtga
     2581 tgacatggag ctgcacagaa tgtcaagaat gcaaagtaaa gaatatcccg tggtccccag
     2641 gagcaccgtg aggcaaaaag tggcctccaa ccacagtcct ttcagcagcg agtctcgagc
     2701 tctctccacc tcatccaatt tggggtccca ataccagtgt gagaatggtg tttccggccc
     2761 ctcccaggac ctcctgcctc cacccaaccc atacccactg ccccaggagc atagccaaat
     2821 ttaccattgt accaagagga aagaggaaga atgttccacc acagaccatc cctataagaa
     2881 gccctacatg gagacatcac ccagtgaaga agattccttc taccgctcta gctatccaca
     2941 gcagcagggc ctgggtgcct cctacaggac agagtcggca cagcggcaag cttgcatgta
     3001 tgccagctct gcgcccccca gcgagcctgt gcccagccta gaggacatca gctgcaacac
     3061 gtggccaagc atgccttcct acagcagctg caccgtcacc accgtgcagc ccatggacag
     3121 gctaccctac cagcacttct ccgctcactt cacctcgggg cccctggtcc ctcggctggc
     3181 tggcatggcc aaccatggct ccccacagct gggagaggga atgttccagc accagacctc
     3241 cgtggcccac cagcctgtgg tcaggcagtg tgggcctcag actggcctgc agtcccctgg
     3301 cacccttcag ccccctgagt tcctctactc tcatggcgtg ccaaggactc tatcccctca
     3361 tcagtaccac tctgtgcacg gagttggcat ggtgccagag tggagcgaca atagctaaag
     3421 tgagcggccg cacttaagtt acgcgtggat caattcactc ctcaggtgca ggctgcctat
     3481 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
     3541 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     3601 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     3661 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     3721 gcaacatatg ccatatgctg gctgccatga acaaaggtgg ctataaagag gtcatcagta
     3781 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     3841 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     3901 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     3961 tcttctctta tgaagatccc tcgacctgca gcccaagctg atcccgggat ttaaatgttt
     4021 aaacgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     4081 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     4141 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     4201 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     4261 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     4321 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4381 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     4441 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4501 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4561 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4621 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4681 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4741 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4801 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4861 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4921 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4981 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5041 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5101 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5161 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5221 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5281 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc cgtgagcgtg
     5341 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5401 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5461 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5521 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5581 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5641 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5701 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5761 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     5821 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5881 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5941 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6001 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6061 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6121 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6181 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6241 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6301 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6361 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6421 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     6481 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     6541 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     6601 gtggaattgt gagcggataa caatttcaca
//
