Basic Vector Information
pUCAG.Gfp vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUCAG.Gfp vector Sequence
LOCUS 40924_45218 5761 bp DNA circular SYN 18-DEC-2018
DEFINITION Shuttle vector pUCAG.Gfp, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5761)
AUTHORS van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der
Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ,
Atsma DE.
TITLE Fibroblasts from human postmyocardial infarction scars acquire
properties of cardiomyocytes after transduction with a recombinant
myocardin gene
JOURNAL FASEB J. 21 (12), 3369-3379 (2007)
PUBMED 17579192
REFERENCE 2 (bases 1 to 5761)
AUTHORS van Tuyn J.
TITLE Direct Submission
JOURNAL Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300
RC, Netherlands
REFERENCE 3 (bases 1 to 5761)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5761)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J.";
date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC,
Netherlands"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5761
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 1..17
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
regulatory 68..448
/label=CMV.IE enhancer
/note="CMV.IE enhancer"
/regulatory_class="enhancer"
enhancer 68..447
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 450..725
/label=chicken beta-actin promoter
intron 726..1734
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
CDS 1827..2543
/label=hrGFP
/note="humanized Renilla green fluorescent protein"
polyA_signal 2730..2785
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3162..3178)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 3652..3756
/label=AmpR promoter
CDS 3757..4614
/label=AmpR
/note="beta-lactamase"
rep_origin 4788..5376
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5664..5685
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5700..5730
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5738..5754
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
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