LOCUS       40924_45218        5761 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pUCAG.Gfp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der 
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ, 
            Atsma DE.
  TITLE     Fibroblasts from human postmyocardial infarction scars acquire 
            properties of cardiomyocytes after transduction with a recombinant 
            myocardin gene
  JOURNAL   FASEB J. 21 (12), 3369-3379 (2007)
  PUBMED    17579192
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 
            RC, Netherlands
REFERENCE   3  (bases 1 to 5761)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5761)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC, 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      68..448
                     /label=CMV.IE enhancer
                     /note="CMV.IE enhancer"
                     /regulatory_class="enhancer"
     enhancer        68..447
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        450..725
                     /label=chicken beta-actin promoter
     intron          726..1734
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1827..2543
                     /label=hrGFP
                     /note="humanized Renilla green fluorescent protein"
     polyA_signal    2730..2785
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3162..3178)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3652..3756
                     /label=AmpR promoter
     CDS             3757..4614
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4788..5376
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5664..5685
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5700..5730
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5738..5754
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcttgttta aacatttaaa tctcgagcca
       61 tggattcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      121 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      181 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      241 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      301 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      361 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      421 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      481 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      541 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
      601 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      661 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      721 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      781 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      841 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      901 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      961 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
     1021 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1081 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1141 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1201 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg
     1261 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1321 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc
     1381 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1441 cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc
     1501 cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1561 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag
     1621 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1681 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1741 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattatc gcatgcctgc
     1801 agagctctag agtcgacgaa ttcaccatgg tgagcaagca gatcctgaag aacaccggcc
     1861 tgcaggagat catgagcttc aaggtgaacc tggagggcgt ggtgaacaac cacgtgttca
     1921 ccatggaggg ctgcggcaag ggcaacatcc tgttcggcaa ccagctggtg cagatccgcg
     1981 tgaccaaggg cgcccccctg cccttcgcct tcgacatcct gagccccgcc ttccagtacg
     2041 gcaaccgcac cttcaccaag taccccgagg acatcagcga cttcttcatc cagagcttcc
     2101 ccgccggctt cgtgtacgag cgcaccctgc gctacgagga cggcggcctg gtggagatcc
     2161 gcagcgacat caacctgatc gaggagatgt tcgtgtaccg cgtggagtac aagggccgca
     2221 acttccccaa cgacggcccc gtgatgaaga agaccatcac cggcctgcag cccagcttcg
     2281 aggtggtgta catgaacgac ggcgtgctgg tgggccaggt gatcctggtg taccgcctga
     2341 acagcggcaa gttctacagc tgccacatgc gcaccctgat gaagagcaag ggcgtggtga
     2401 aggacttccc cgagtaccac ttcatccagc accgcctgga gaagacctac gtggaggacg
     2461 gcggcttcgt ggagcagcac gagaccgcca tcgcccagct gaccagcctg ggcaagcccc
     2521 tgggcagcct gcacgagtgg gtgtaatagc tcgagcggcc gcacttaagt tacgcgtgga
     2581 tcaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     2641 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     2701 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     2761 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     2821 acatcagaat gagtatttgg tttagagttt ggcaacatat gccatatgct ggctgccatg
     2881 aacaaaggtg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     2941 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     3001 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     3061 tctcctgact actcccagtc atagctgtcc ctcttctctt atgaagatcc ctcgacctgc
     3121 agcccaagct gatcccggga tttaaatgtt taaacgaatt cactggccgt cgttttacaa
     3181 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     3241 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     3301 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3361 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3421 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     3481 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     3541 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     3601 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3661 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3721 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3781 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3841 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3901 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3961 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4021 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4081 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4141 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4201 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4261 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4321 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4381 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4441 attgctgata aatctggagc ccgtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4501 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4561 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4621 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4681 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4741 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4801 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4861 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4921 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4981 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5041 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5101 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5161 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5221 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5281 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5341 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5401 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5461 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5521 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     5581 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     5641 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     5701 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     5761 a
//
