Basic Vector Information
- Vector Name:
- pUC57-Ps12-turbo-RFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3527 bp
- Type:
- Fluorescent tagging vector
- Replication origin:
- ori
- Source/Author:
- Norris MH, Kang Y, Hoang TT.
pUC57-Ps12-turbo-RFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUC57-Ps12-turbo-RFP vector Sequence
LOCUS 40924_45183 3527 bp DNA circular SYN 18-DEC-2018
DEFINITION Fluorescent tagging vector pUC57-Ps12-turbo-RFP, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3527)
AUTHORS Norris MH, Kang Y, Hoang TT.
TITLE Stable site-specific fluorescent tagging tools optimized for
burkholderia species
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3527)
AUTHORS Norris MH, Kang Y, Hoang TT.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2010) Microbiology, University of Hawaii at Manoa,
2538 Mccarthy Mall Snyder 310, Honolulu, HI 96822, USA
REFERENCE 3 (bases 1 to 3527)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3527)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(22-APR-2010) Microbiology, University of Hawaii at Manoa, 2538
Mccarthy Mall Snyder 310, Honolulu, HI 96822, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3527
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 379..395
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS 523..1215
/codon_start=1
/label=TurboRFP
/note="red fluorescent protein from Entacmaea quadricolor"
/translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
DLPSKLGHR"
primer_bind complement(1306..1322)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(1330..1346)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1354..1384)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(1399..1420)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1708..2296)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2470..3327)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3328..3432)
/label=AmpR promoter
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