LOCUS       40924_45183        3527 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fluorescent tagging vector pUC57-Ps12-turbo-RFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Norris MH, Kang Y, Hoang TT.
  TITLE     Stable site-specific fluorescent tagging tools optimized for 
            burkholderia species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Norris MH, Kang Y, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2010) Microbiology, University of Hawaii at Manoa,
            2538 Mccarthy Mall Snyder 310, Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 3527)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3527)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2010) Microbiology, University of Hawaii at Manoa, 2538 
            Mccarthy Mall Snyder 310, Honolulu, HI 96822, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             523..1215
                     /codon_start=1
                     /label=TurboRFP
                     /note="red fluorescent protein from Entacmaea quadricolor"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     primer_bind     complement(1306..1322)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1330..1346)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1354..1384)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1399..1420)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1708..2296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2470..3327)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3328..3432)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tccaattcta gaagctgttg actcgcttgg gattttcgga atatcatgcc
      481 gggttccggg accatgggat cgagcttcga aaggacaagc atatgagcga gctgatcaag
      541 gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc
      601 acgtccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgaagat caaggtggtc
      661 gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc
      721 aaagccttca tcaaccacac ccagggcatc cccgacttct ttaagcagtc cttccctgag
      781 ggcttcacgt gggagagaat caccacgtac gaagacgggg gcgtgctgac cgcgacccag
      841 gacaccagct tccagaacgg ctgcatcatc tacaacgtca agatcaacgg ggtgaacttc
      901 ccatccaacg gccctgtgat gcagaagaaa acccgcggct gggaggccaa caccgagatg
      961 ctgtaccccg ccgacggcgg cctgagaggc cacagccaga tggccctgaa gctcgtgggc
     1021 gggggctacc tgcactgctc cttcaagacc acctacagat ccaagaagcc cgccaagaac
     1081 ctcaagatgc ccggcttcca cttcgtggac cacagactgg aaagaatcaa ggaggccgac
     1141 aaggagacct acgtcgagca gcacgagatg gcggtggcca agtattgcga cctccccagc
     1201 aagctggggc accggtgatt taagaaggag atataccatg gaaattggat cggatcccgg
     1261 gcccgtcgac tgcagaggcc tgcatgcaag cttggcgtaa tcatggtcat agctgtttcc
     1321 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     1381 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1441 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1501 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1561 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1621 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1681 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1741 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1801 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1861 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1921 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1981 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2041 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2101 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2161 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2221 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2281 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2341 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2401 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2461 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2521 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2581 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2641 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2701 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2761 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2821 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2881 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2941 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3001 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3061 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3121 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3181 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3241 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3301 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3361 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3421 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     3481 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
