pUC18T-mini-Tn7T-Zeo vector (V002407)

LOCUS       40924_45098        4289 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC18T-mini-Tn7T-Zeo, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Choi K-H., Schweizer HP.
  TITLE     Mini-Tn7 insertion in bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Choi K-H., Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2006) Microbiology, Immunology and Pathology, 
            Colorado State University, 1682 Campus Delivery, Fort Collins, CO 
            80523-1682, USA
REFERENCE   3  (bases 1 to 4289)
  AUTHORS   Choi K-H., Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2010) Microbiology, Immunology and Pathology, 
            Colorado State University, 1682 Campus delivery, Ft. Collins, CO 
            80523, USA
REFERENCE   4  (bases 1 to 4289)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4289)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2006) Microbiology, Immunology and Pathology, Colorado State
            University, 1682 Campus Delivery, Fort Collins, CO 80523-1682, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (25-MAY-2010) Microbiology, Immunology and Pathology, Colorado State
            University, 1682 Campus delivery, Ft. Collins, CO 80523, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On May 25, 2010 this sequence version replaced DQ493876.1.
FEATURES             Location/Qualifiers
     source          1..4289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /label=n7R
                     /note="right end of Tn7"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    958..1005
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1119..1490)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     promoter        complement(1509..1556)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     misc_feature    1646..1693
                     /note="FRT; Flp recombinase target site"
     protein_bind    1646..1693
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    1720..1797
                     /note="MCS; multiple cloning site"
     mobile_element  complement(1806..1971)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(2130..2238)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(2470..3058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3232..4089)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4090..4194)
                     /label=AmpR promoter