Basic Vector Information
pUC18T-mini-Tn7T-Zeo vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUC18T-mini-Tn7T-Zeo vector Sequence
LOCUS 40924_45098 4289 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pUC18T-mini-Tn7T-Zeo, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4289)
AUTHORS Choi K-H., Schweizer HP.
TITLE Mini-Tn7 insertion in bacteria
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4289)
AUTHORS Choi K-H., Schweizer HP.
TITLE Direct Submission
JOURNAL Submitted (13-APR-2006) Microbiology, Immunology and Pathology,
Colorado State University, 1682 Campus Delivery, Fort Collins, CO
80523-1682, USA
REFERENCE 3 (bases 1 to 4289)
AUTHORS Choi K-H., Schweizer HP.
TITLE Direct Submission
JOURNAL Submitted (25-MAY-2010) Microbiology, Immunology and Pathology,
Colorado State University, 1682 Campus delivery, Ft. Collins, CO
80523, USA
REFERENCE 4 (bases 1 to 4289)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 4289)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-APR-2006) Microbiology, Immunology and Pathology, Colorado State
University, 1682 Campus Delivery, Fort Collins, CO 80523-1682, USA"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(25-MAY-2010) Microbiology, Immunology and Pathology, Colorado State
University, 1682 Campus delivery, Ft. Collins, CO 80523, USA"
COMMENT SGRef: number: 4; type: "Journal Article"
COMMENT On May 25, 2010 this sequence version replaced DQ493876.1.
FEATURES Location/Qualifiers
source 1..4289
/mol_type="other DNA"
/organism="synthetic DNA construct"
mobile_element 382..580
/mobile_element_type="transposon:Tn7R"
/label=n7R
/note="right end of Tn7"
primer_bind 585..601
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
terminator 629..723
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
terminator 826..912
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
protein_bind 958..1005
/label=FRT
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS complement(1119..1490)
/label=BleoR
/note="antibiotic-binding protein"
promoter complement(1509..1556)
/label=EM7 promoter
/note="synthetic bacterial promoter"
misc_feature 1646..1693
/note="FRT; Flp recombinase target site"
protein_bind 1646..1693
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
misc_feature 1720..1797
/note="MCS; multiple cloning site"
mobile_element complement(1806..1971)
/label=Tn7L
/note="mini-Tn7 element (left end of the Tn7 transposon)"
oriT complement(2130..2238)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
rep_origin complement(2470..3058)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3232..4089)
/label=AmpR
/note="beta-lactamase"
promoter complement(4090..4194)
/label=AmpR promoter
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