LOCUS       40924_45098        4289 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC18T-mini-Tn7T-Zeo, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Choi K-H., Schweizer HP.
  TITLE     Mini-Tn7 insertion in bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Choi K-H., Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2006) Microbiology, Immunology and Pathology, 
            Colorado State University, 1682 Campus Delivery, Fort Collins, CO 
            80523-1682, USA
REFERENCE   3  (bases 1 to 4289)
  AUTHORS   Choi K-H., Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2010) Microbiology, Immunology and Pathology, 
            Colorado State University, 1682 Campus delivery, Ft. Collins, CO 
            80523, USA
REFERENCE   4  (bases 1 to 4289)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4289)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2006) Microbiology, Immunology and Pathology, Colorado State
            University, 1682 Campus Delivery, Fort Collins, CO 80523-1682, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (25-MAY-2010) Microbiology, Immunology and Pathology, Colorado State
            University, 1682 Campus delivery, Ft. Collins, CO 80523, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On May 25, 2010 this sequence version replaced DQ493876.1.
FEATURES             Location/Qualifiers
     source          1..4289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /label=n7R
                     /note="right end of Tn7"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    958..1005
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1119..1490)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     promoter        complement(1509..1556)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     misc_feature    1646..1693
                     /note="FRT; Flp recombinase target site"
     protein_bind    1646..1693
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    1720..1797
                     /note="MCS; multiple cloning site"
     mobile_element  complement(1806..1971)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(2130..2238)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(2470..3058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3232..4089)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4090..4194)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gtacctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa taattctaga taattcggct tatctctgat
     1081 gttacattgc acaagataaa aatatatcat catgatcagt cctgctcctc ggccacgaag
     1141 tgcacgcagt tgccggccgg gtcgcgcagg gcgaactccc gcccccacgg ctgctcgccg
     1201 atctcggtca tggccggccc ggaggcgtcc cggaagttcg tggacacgac ctccgaccac
     1261 tcggcgtaca gctcgtccag gccgcgcacc cacacccagg ccagggtgtt gtccggcacc
     1321 acctggtcct ggaccgcgct gatgaacagg gtcacgtcgt cccggaccac accggcgaag
     1381 tcgtcctcca cgaagtcccg ggagaacccg agccggtcgg tccagaactc gaccgctccg
     1441 gcgacgtcgc gcgcggtgag caccggaacg gcactggtca acttggccat ggtttagttc
     1501 ctcaccttgt cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt
     1561 gctgatcatg accaaaatcc cttaacgtga gttacgcgtc gaagccgaaa tccattgctg
     1621 ttgacaaagg gaatcagggg atcttgaagt tcctattccg aagttcctat tctctagaaa
     1681 gtataggaac ttcagagcgc ttttgaagct aattcgatca tgcatgagct cactagtgga
     1741 tcccccgggc tgcaggaatt cctcgagaag cttgggcccg gtacctcgcg aaggccttgc
     1801 aggccaacca gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat
     1861 tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt
     1921 aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat
     1981 ttttcttcct gttatgtttg ggcgctgcat taatgaatcg gccaacgcgc ggggagaggc
     2041 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg acccgctgcg ctcggtcgtt
     2101 cggctgcggc gagcggtatc agagcttatc ggccagcctc gcagagcagg attcccgttg
     2161 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     2221 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     2281 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     2341 atgaccccga agcagggtta tgcagcggaa agtatacctt aaggaatccc ctgataacgc
     2401 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2461 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcgag
     2521 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2581 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2641 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2701 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2761 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2821 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2881 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     2941 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3001 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3061 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3121 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3181 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3241 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3301 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3361 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3421 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3481 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3541 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3601 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3661 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3721 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3781 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3841 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3901 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3961 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4021 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4081 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4141 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4201 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     4261 aaataggcgt atcacgaggc cctttcgtc
//
