Basic Vector Information
- Vector Name:
- pUC18-mini-Tn7T-Tp
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4136 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Choi K-H., Gaynor JB, White KG, Karkhoff-Schweizer RR, Schweizer HP.
- Promoter:
- Pc
pUC18-mini-Tn7T-Tp vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUC18-mini-Tn7T-Tp vector Sequence
LOCUS 40924_45008 4136 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pUC18-mini-Tn7T-Tp, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4136)
AUTHORS Choi K-H., Gaynor JB, White KG, Karkhoff-Schweizer RR, Schweizer HP.
TITLE A Tn7-based universal bacterial cloning and expression system
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4136)
AUTHORS Choi K-H., Gaynor JB, White KG, Karkhoff-Schweizer RR, Schweizer HP.
TITLE Direct Submission
JOURNAL Submitted (27-AUG-2004) Microbiology, Immunology and Pathology,
Colorado State University, Fort Collins, CO 80523, USA
REFERENCE 3 (bases 1 to 4136)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4136)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(27-AUG-2004) Microbiology, Immunology and Pathology, Colorado State
University, Fort Collins, CO 80523, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4136
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 382..580
/label=Tn7R, right end of Tn7
/note="Tn7R, right end of Tn7"
primer_bind 585..601
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
terminator 629..723
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
terminator 826..912
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
protein_bind 964..1011
/label=FRT
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS complement(1076..1309)
/codon_start=1
/label=TpR
/note="E. coli plasmid-associated dihydrofolate reductase"
/translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
promoter complement(1636..1664)
/label=Pc promoter
/note="class 1 integron promoter"
misc_feature 1716..1763
/label=FRT, Flp recombinase target site
/note="FRT, Flp recombinase target site"
protein_bind 1716..1763
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
misc_feature 1746..1873
/label=multiple cloning site
/note="multiple cloning site"
mobile_element complement(1882..2047)
/label=Tn7L
/note="mini-Tn7 element (left end of the Tn7 transposon)"
rep_origin complement(2317..2905)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3079..3936)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3937..4041)
/label=AmpR promoter
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