LOCUS       40924_45008        4136 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC18-mini-Tn7T-Tp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Choi K-H., Gaynor JB, White KG, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     A Tn7-based universal bacterial cloning and expression system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Choi K-H., Gaynor JB, White KG, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2004) Microbiology, Immunology and Pathology, 
            Colorado State University, Fort Collins, CO 80523, USA
REFERENCE   3  (bases 1 to 4136)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4136)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-AUG-2004) Microbiology, Immunology and Pathology, Colorado State
            University, Fort Collins, CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   382..580
                     /label=Tn7R, right end of Tn7
                     /note="Tn7R, right end of Tn7"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    964..1011
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1076..1309)
                     /codon_start=1
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     promoter        complement(1636..1664)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1716..1763
                     /label=FRT, Flp recombinase target site
                     /note="FRT, Flp recombinase target site"
     protein_bind    1716..1763
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    1746..1873
                     /label=multiple cloning site
                     /note="multiple cloning site"
     mobile_element  complement(1882..2047)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      complement(2317..2905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3079..3936)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3937..4041)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgagctcg aattggggat
      961 cttgaagtac ctattccgaa gttcctattc tctagaaagt ataggaactt cagagcgctt
     1021 ttgaagctga tgtgcttaaa aacttactca atggaataat tctagataat tcttaggcca
     1081 cacgttcaag tgcagccaca ggataaattt gcactgagcc tgggtgggat tcggactcga
     1141 ccgcatagcc ttcaggagtg agttttgtgc aataccaacc gacgacttga ccctgccaag
     1201 cggcaccaga tttcttgcgt acgcgatccc ctaagccaaa ggtggcactc aggggaagcg
     1261 caaactgccc tgcaacggga gcgttggctt catcgctact ttgacccatg tcgaatcctt
     1321 cttgtgaatc tattatggcg acaagcaaat cgagctctga ctgcctaccc cacaacaact
     1381 actagaaagc accagcacaa cggctgccta actttgtttt agggcgacta ccctgctgcg
     1441 taacatcgtt gctgttccat aacatcaaac atcgacccac ggcgtaacgc gcttagctag
     1501 cttggatgcc cgaggctaga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc
     1561 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     1621 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgaatta
     1681 tccattgctg ttgacaaagg gaatcagggg atcttgaagt tcctattccg aagttcctat
     1741 tctctagaaa gtataggaac ttcagagcgc ttttgaagct aattcgagct cgatcatgca
     1801 tgagctcact agtggatccc ccgggctgca ggaattcctc gagaagcttg ggcccggtac
     1861 ctcgcgaagg ccttgcaggc caaccagata agtgaaatct agttccaaac tattttgtca
     1921 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     1981 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     2041 gcccacagcg gggcattttt cttcctgtta tgtttgggcg ctgcattaat gaatcggcca
     2101 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2161 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2221 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2281 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2341 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2401 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2461 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2521 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2581 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2641 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2701 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     2761 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2821 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2881 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2941 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3001 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3061 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3121 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3181 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3241 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3301 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3361 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3421 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3481 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3541 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3601 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3661 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3721 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3781 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3841 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3901 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3961 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4021 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     4081 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
