Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V002444 | pUC-GM-INT | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
pUC-GM-INT vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Schweizer HD. Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis. Biotechniques. 1993 Nov;15(5):831-4
pUC-GM-INT vector Sequence
LOCUS 40924_44883 5646 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pUC-GM-INT, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5646) AUTHORS Schweizer HD. TITLE Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis JOURNAL BioTechniques 15 (5), 831-834 (1993) PUBMED 8267974 REFERENCE 2 (bases 1 to 5646) AUTHORS Mahenthiralingam E, Marklund BI, Brooks LA, Smith DA, Bancroft GJ, Stokes RW. TITLE Site-directed mutagenesis of the 19-kilodalton lipoprotein antigen reveals No essential role for the protein in the growth and virulence of Mycobacterium intracellulare JOURNAL Infect. Immun. 66 (8), 3626-3634 (1998) PUBMED 9673242 REFERENCE 3 (bases 1 to 5646) AUTHORS Mahenthiralingam E, Marklund B-I., Brooks LA, Smith DA, Bancroft GJ, Stokes RW. TITLE Direct Submission JOURNAL Submitted (16-SEP-1997) Pediatrics, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada REFERENCE 4 (bases 1 to 5646) TITLE Direct Submission REFERENCE 5 (bases 1 to 5646) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "BioTechniques"; date: "1993"; volume: "15"; issue: "5"; pages: "831-834" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Infect. Immun."; date: "1998"; volume: "66"; issue: "8"; pages: "3626-3634" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (16-SEP-1997) Pediatrics, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada" COMMENT SGRef: number: 4; type: "Journal Article" FEATURES Location/Qualifiers source 1..5646 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature complement(399..455) /label=MCS /note="pUC18/19 multiple cloning site" promoter 453..481 /label=Pc promoter /note="class 1 integron promoter" CDS 670..1200 /label=GmR /note="gentamycin acetyltransferase" misc_feature 1662..1740 /note="encodes mycobacteriophage attachment site, attP core" CDS 1872..2984 /gene="33" /label=33 /note="Integrase from Mycobacterium phage L5. Accession#: P22884" primer_bind complement(3425..3441) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(3449..3465) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3473..3503) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3518..3539) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3827..4415) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4589..5446) /label=AmpR /note="beta-lactamase" promoter complement(5447..5551) /label=AmpR promoter