pUC-GM-INT vector (V002444)

Price Information

Cat No. Plasmid Name Availability Add to cart
V002444 pUC-GM-INT In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

      • Vector Name:
      • pUC-GM-INT
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5646 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Schweizer HD.

pUC-GM-INT vector Vector Map

pUC-GM-INT5646 bp60012001800240030003600420048005400M13 fwdMCSGmRencodes mycobacteriophage attachment site, attP core33M13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

  • Schweizer HD. Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis. Biotechniques. 1993 Nov;15(5):831-4

pUC-GM-INT vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_44883        5646 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC-GM-INT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Schweizer HD.
  TITLE     Small broad-host-range gentamycin resistance gene cassettes for 
            site-specific insertion and deletion mutagenesis
  JOURNAL   BioTechniques 15 (5), 831-834 (1993)
  PUBMED    8267974
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Mahenthiralingam E, Marklund BI, Brooks LA, Smith DA, Bancroft GJ, 
            Stokes RW.
  TITLE     Site-directed mutagenesis of the 19-kilodalton lipoprotein antigen 
            reveals No essential role for the protein in the growth and 
            virulence of Mycobacterium intracellulare
  JOURNAL   Infect. Immun. 66 (8), 3626-3634 (1998)
  PUBMED    9673242
REFERENCE   3  (bases 1 to 5646)
  AUTHORS   Mahenthiralingam E, Marklund B-I., Brooks LA, Smith DA, Bancroft GJ,
            Stokes RW.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) Pediatrics, University of British Columbia, 
            950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada
REFERENCE   4  (bases 1 to 5646)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5646)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1993"; volume: "15"; issue: "5"; pages: 
            "831-834"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Infect. 
            Immun."; date: "1998"; volume: "66"; issue: "8"; pages: "3626-3634"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (16-SEP-1997) Pediatrics, University of British Columbia, 950 West 
            28th Avenue, Vancouver, BC V5Z 4H4, Canada"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(399..455)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        453..481
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             670..1200
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     misc_feature    1662..1740
                     /note="encodes mycobacteriophage attachment site, attP
                     core"
     CDS             1872..2984
                     /gene="33"
                     /label=33
                     /note="Integrase from Mycobacterium phage L5. Accession#: 
                     P22884"
     primer_bind     complement(3425..3441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3449..3465)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3473..3503)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3518..3539)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3827..4415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4589..5446)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5447..5551)
                     /label=AmpR promoter