Cart 2

pUC-GM-INT vector (V002444#)

Basic Vector Information

Basic Vector Information
Vector Name pUC-GM-INT Antibiotic Resistance Ampicillin
Length 5646 bp Type Cloning vector
Source Schweizer HD.

pUC-GM-INT vector Vector Map

M13 fwd Pc promoter aacC1 encodes mycobacteriophage attachment site, attP core L5-integrase M13 rev lac operator lac promoter CAP binding site ori bla AmpR promoter

Plasmid Resuspension protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUC-GM-INT vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                5646 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC-GM-INT, complete sequence.
ACCESSION   AF025392
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Schweizer HD.
  TITLE     Small broad-host-range gentamycin resistance gene cassettes for 
            site-specific insertion and deletion mutagenesis
  JOURNAL   BioTechniques 15 (5), 831-834 (1993)
  PUBMED    8267974
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Mahenthiralingam E, Marklund BI, Brooks LA, Smith DA, Bancroft GJ, 
            Stokes RW.
  TITLE     Site-directed mutagenesis of the 19-kilodalton lipoprotein antigen 
            reveals No essential role for the protein in the growth and 
            virulence of Mycobacterium intracellulare
  JOURNAL   Infect. Immun. 66 (8), 3626-3634 (1998)
  PUBMED    9673242
REFERENCE   3  (bases 1 to 5646)
  AUTHORS   Mahenthiralingam E, Marklund B-I., Brooks LA, Smith DA, Bancroft GJ,
            Stokes RW.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) Pediatrics, University of British Columbia, 
            950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada
REFERENCE   4  (bases 1 to 5646)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Cloning vector pUC-GM-INT"
                     /mol_type="genomic DNA"
                     /note="L5-integrase based positive selection mycobacterial 
                     cloning cassette"
                     /db_xref="taxon:67802"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        453..481
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             670..1203
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyl transferase"
                     /function="gentamycin-resistance"
                     /label=aacC1
                     /protein_id="AAC31620.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            670..1203
                     /gene="aacC1"
                     /label=aacC1
     misc_feature    1662..1740
                     /note="encodes mycobacteriophage attachment site, attP 
                     core"
     CDS             1989..2987
                     /codon_start=1
                     /product="L5-integrase"
                     /label=L5-integrase
                     /note="mycobacteriophage integrase"
                     /protein_id="AAC31622.1"
                     /translation="MDAEAWLAGEKRLIEMETWTPPQDRAKKAAASAITLEEYTRKWLV
                     ERDLADGTRDLYSGHAERRIYPVLGEVAVTEMTPALVRAWWAGMGRKHPTARRHAYNVL
                     RAVMNTAVEDKLIAENPCRIEQKAADERDVEALTPEELDIVAAEIFEHYRIAAYILAWT
                     SLRFGELIELRRKDIVDDGMTMKLRVRRGASRVGNKIVVGNAKTVRSKRPVTVPPHVAE
                     MIRAHMKDRTKMNKGPEAFLVTTTQGNRLSKSAFTKSLKRGYAKIGRPELRIHDLRAVG
                     ATFAAQAGATTKELMARLGHTTPRMAMKYQMASEARDEAIAEAMSKLAKTS"
     primer_bind     complement(3425..3441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3449..3465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3473..3503)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3518..3539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3827..4415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4586..5446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAC31621.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4586..5446)
                     /gene="bla"
                     /label=bla
     promoter        complement(5447..5551)
                     /gene="bla"
                     /label=AmpR promoter

This page is informational only.