LOCUS       V002444                 5646 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002444
VERSION     V002444
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Schweizer HD.
  TITLE     Small broad-host-range gentamycin resistance gene cassettes for
            site-specific insertion and deletion mutagenesis
  JOURNAL   BioTechniques 15 (5), 831-834 (1993)
   PUBMED   8267974
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Mahenthiralingam E, Marklund BI, Brooks LA, Smith DA, Bancroft GJ,
            Stokes RW.
  TITLE     Site-directed mutagenesis of the 19-kilodalton lipoprotein antigen
            reveals No essential role for the protein in the growth and
            virulence of Mycobacterium intracellulare
  JOURNAL   Infect. Immun. 66 (8), 3626-3634 (1998)
   PUBMED   9673242
REFERENCE   3  (bases 1 to 5646)
  AUTHORS   Mahenthiralingam E, Marklund B-I., Brooks LA, Smith DA, Bancroft GJ,
            Stokes RW.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) Pediatrics, University of British Columbia,
            950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada
REFERENCE   4  (bases 1 to 5646)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5646)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "BioTechniques"; date: "1993"; volume: "15"; issue: "5"; pages:
            "831-834"
            SGRef: number: 2; type: "Journal Article"; journalName: "Infect.
            Immun."; date: "1998"; volume: "66"; issue: "8"; pages: "3626-3634"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (16-SEP-1997) Pediatrics, University of British Columbia, 950 West
            28th Avenue, Vancouver, BC V5Z 4H4, Canada"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(399..455)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     promoter        453..481
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             670..1200
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     misc_feature    1662..1740
                     /note="encodes mycobacteriophage attachment site, attP
                     core"
     CDS             1872..2984
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     primer_bind     complement(3425..3441)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3449..3465)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3473..3503)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3518..3539)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3827..4415)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4589..5446)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5447..5551)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg aattgacata agcctgttcg gttcgtaaac
      481 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
      541 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt gtacagtcta
      601 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
      661 gcagcaacga tgttacgcag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
      721 aagttaggtg gctcaagtat gggcatcatt cgcacatgta ggctcggccc tgaccaagtc
      781 aaatccatgc gggctgctct tgatcttttc ggtcgtgagt tcggagacgt agccacctac
      841 tcccaacatc agccggactc cgattacctc gggaacttgc tccgtagtaa gacattcatc
      901 gcgcttgctg ccttcgacca agaagcggtt gttggcgctc tcgcggctta cgttctgccc
      961 aggtttgagc agccgcgtag tgagatctat atctatgatc tcgcagtctc cggcgagcac
     1021 cggaggcagg gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt
     1081 ggtgcttatg tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat
     1141 acaaagttgg gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc
     1201 taacaattcg ttcaagccga gatcggcttc ccggccgcgg agttgttcgg taaattgtca
     1261 caacgccgcg gccaattcga gctcggtacc cggggatcct ctagagtcga ccaccaaggg
     1321 caccatctct gcttgggcca ccccgttggc cgcagccagc tcgctgagag ccgtgaacga
     1381 cagggcgaac gccagcccgc cgacggcgag ggttccgacc gctgcaactc ccggtgcaac
     1441 cttgtcccgg tctattctct tcactgcacc agctccaatc tggtgtgaat gcccctcgtc
     1501 tgttcgcgca ggcggggggc tctattcgtt tgtcagcatc gaaagtagcc agatcaggga
     1561 tgcgttgcaa ccgcgtatgc ccaggtcaga agagtcgcac aagagttgca gacccctgga
     1621 aagaaaaatg gccagagggc gaaaacaccc tctgaccagc ggagcgggcg acgggaatcg
     1681 aacccgcgta gctagtttgg aagaatgggt gtctgccgac cacatatggg ccggtcaaga
     1741 taggttttta ccccctctcg gctgcatcct ctaagtggaa agaaattgca ggtcgtagaa
     1801 gcgcgttgaa gcctgagagt tgcacaggag ttgcaacccg gtagccttgt tcacgacgag
     1861 aggagaccta gttggcacgt cgcggatggg gatcgctgaa gactcagcgc agcgggagga
     1921 tccaagcctc atacgtcaac ccgcaggacg gtgtgaggta ctacgcgctg cagacctacg
     1981 acaacaagat ggacgccgaa gcctggctcg cgggcgagaa gcggctcatc gagatggaga
     2041 cctggacccc tccacaggac cgggcgaaga aggcagccgc cagcgccatc acgctggagg
     2101 agtacacccg gaagtggctc gtggagcgcg acctcgcaga cggcaccagg gatctgtaca
     2161 gcgggcacgc ggagcgccgc atctacccgg tgctaggtga agtggcggtc acagagatga
     2221 cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag gaagcacccg actgcccgcc
     2281 ggcatgccta caacgtcctc cgggcggtga tgaacacagc ggtcgaggac aagctgatcg
     2341 cagagaaccc gtgccggatc gagcagaagg cagccgatga gcgcgacgta gaggcgctga
     2401 cgcctgagga gctggacatc gtcgccgctg agatcttcga gcactaccgg atcgcggcat
     2461 acatcctggc gtggacgagc ctccggttcg gagagctgat cgagcttcgc cgcaaggaca
     2521 tcgtggacga cggcatgacg atgaagctcc gggtgcgccg tggcgcttcc cgcgtgggga
     2581 acaagatcgt cgttggcaac gccaagaccg tccggtcgaa gcgtcctgtg acggttccgc
     2641 ctcacgtcgc ggagatgatc cgagcgcaca tgaaggaccg tacgaagatg aacaagggcc
     2701 ccgaggcatt cctggtgacc acgacgcagg gcaaccggct gtcgaagtcc gcgttcacca
     2761 agtcgctgaa gcgtggctac gccaagatcg gtcggccgga actccgcatc cacgacctcc
     2821 gcgctgtcgg cgctacgttc gccgctcagg caggtgcgac gaccaaggag ctgatggccc
     2881 gtctcggtca cacgactcct aggatggcga tgaagtacca gatggcgtct gaggcccgcg
     2941 acgaggctat cgctgaggcg atgtccaagc tggccaagac ctcctgaaac gcaaaaagcc
     3001 cccctcccaa ggacactgag tcctaaagag gggggtttct tgtcagtacg cgaagaacca
     3061 cgcctggccg cgagcgccag caccgccgct ctgtgcggag acctgggcac cagccccgcc
     3121 gccgccagga gcattgccgt tcccgccagc tgagttctgt tgtgcgccgc ctatgtagag
     3181 ctggtcgttg taggtccgat ctccaggcga ctttccggcg acgctgagga tgtcgatcac
     3241 agagcctccg ggaccgccgg ttgcggtcaa acctgaccat ccgacagcgg acgccgtggt
     3301 gtttcctcca gggcctccgg ccttgcctga gaatacagag ccagctcccg ctgcgcctcc
     3361 agctccgacg agcccggtga tcgtcttggt cgacctgcag gcatgcaagc ttggcgtaat
     3421 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3481 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3541 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3601 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3661 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3721 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3781 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3841 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3901 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3961 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4021 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4081 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4141 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4201 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4261 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4321 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4381 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4441 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4501 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4561 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4621 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4681 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4741 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4801 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4861 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4921 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4981 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5041 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5101 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5161 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5221 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5281 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5341 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5401 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5461 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5521 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     5581 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     5641 ttcgtc
//
