LOCUS 40924_44883 5646 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pUC-GM-INT, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5646) AUTHORS Schweizer HD. TITLE Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis JOURNAL BioTechniques 15 (5), 831-834 (1993) PUBMED 8267974 REFERENCE 2 (bases 1 to 5646) AUTHORS Mahenthiralingam E, Marklund BI, Brooks LA, Smith DA, Bancroft GJ, Stokes RW. TITLE Site-directed mutagenesis of the 19-kilodalton lipoprotein antigen reveals No essential role for the protein in the growth and virulence of Mycobacterium intracellulare JOURNAL Infect. Immun. 66 (8), 3626-3634 (1998) PUBMED 9673242 REFERENCE 3 (bases 1 to 5646) AUTHORS Mahenthiralingam E, Marklund B-I., Brooks LA, Smith DA, Bancroft GJ, Stokes RW. TITLE Direct Submission JOURNAL Submitted (16-SEP-1997) Pediatrics, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada REFERENCE 4 (bases 1 to 5646) TITLE Direct Submission REFERENCE 5 (bases 1 to 5646) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "BioTechniques"; date: "1993"; volume: "15"; issue: "5"; pages: "831-834" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Infect. Immun."; date: "1998"; volume: "66"; issue: "8"; pages: "3626-3634" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (16-SEP-1997) Pediatrics, University of British Columbia, 950 West 28th Avenue, Vancouver, BC V5Z 4H4, Canada" COMMENT SGRef: number: 4; type: "Journal Article" FEATURES Location/Qualifiers source 1..5646 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature complement(399..455) /label=MCS /note="pUC18/19 multiple cloning site" promoter 453..481 /label=Pc promoter /note="class 1 integron promoter" CDS 670..1200 /label=GmR /note="gentamycin acetyltransferase" misc_feature 1662..1740 /note="encodes mycobacteriophage attachment site, attP core" CDS 1872..2984 /gene="33" /label=33 /note="Integrase from Mycobacterium phage L5. Accession#: P22884" primer_bind complement(3425..3441) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(3449..3465) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3473..3503) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3518..3539) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3827..4415) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4589..5446) /label=AmpR /note="beta-lactamase" promoter complement(5447..5551) /label=AmpR promoter ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg 421 actctagagg atccccgggt accgagctcg aattgacata agcctgttcg gttcgtaaac 481 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg 541 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt gtacagtcta 601 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga 661 gcagcaacga tgttacgcag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca 721 aagttaggtg gctcaagtat gggcatcatt cgcacatgta ggctcggccc tgaccaagtc 781 aaatccatgc gggctgctct tgatcttttc ggtcgtgagt tcggagacgt agccacctac 841 tcccaacatc agccggactc cgattacctc gggaacttgc tccgtagtaa gacattcatc 901 gcgcttgctg ccttcgacca agaagcggtt gttggcgctc tcgcggctta cgttctgccc 961 aggtttgagc agccgcgtag tgagatctat atctatgatc tcgcagtctc cggcgagcac 1021 cggaggcagg gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt 1081 ggtgcttatg tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat 1141 acaaagttgg gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc 1201 taacaattcg ttcaagccga gatcggcttc ccggccgcgg agttgttcgg taaattgtca 1261 caacgccgcg gccaattcga gctcggtacc cggggatcct ctagagtcga ccaccaaggg 1321 caccatctct gcttgggcca ccccgttggc cgcagccagc tcgctgagag ccgtgaacga 1381 cagggcgaac gccagcccgc cgacggcgag ggttccgacc gctgcaactc ccggtgcaac 1441 cttgtcccgg tctattctct tcactgcacc agctccaatc tggtgtgaat gcccctcgtc 1501 tgttcgcgca ggcggggggc tctattcgtt tgtcagcatc gaaagtagcc agatcaggga 1561 tgcgttgcaa ccgcgtatgc ccaggtcaga agagtcgcac aagagttgca gacccctgga 1621 aagaaaaatg gccagagggc gaaaacaccc tctgaccagc ggagcgggcg acgggaatcg 1681 aacccgcgta gctagtttgg aagaatgggt gtctgccgac cacatatggg ccggtcaaga 1741 taggttttta ccccctctcg gctgcatcct ctaagtggaa agaaattgca ggtcgtagaa 1801 gcgcgttgaa gcctgagagt tgcacaggag ttgcaacccg gtagccttgt tcacgacgag 1861 aggagaccta gttggcacgt cgcggatggg gatcgctgaa gactcagcgc agcgggagga 1921 tccaagcctc atacgtcaac ccgcaggacg gtgtgaggta ctacgcgctg cagacctacg 1981 acaacaagat ggacgccgaa gcctggctcg cgggcgagaa gcggctcatc gagatggaga 2041 cctggacccc tccacaggac cgggcgaaga aggcagccgc cagcgccatc acgctggagg 2101 agtacacccg gaagtggctc gtggagcgcg acctcgcaga cggcaccagg gatctgtaca 2161 gcgggcacgc ggagcgccgc atctacccgg tgctaggtga agtggcggtc acagagatga 2221 cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag gaagcacccg actgcccgcc 2281 ggcatgccta caacgtcctc cgggcggtga tgaacacagc ggtcgaggac aagctgatcg 2341 cagagaaccc gtgccggatc gagcagaagg cagccgatga gcgcgacgta gaggcgctga 2401 cgcctgagga gctggacatc gtcgccgctg agatcttcga gcactaccgg atcgcggcat 2461 acatcctggc gtggacgagc ctccggttcg gagagctgat cgagcttcgc cgcaaggaca 2521 tcgtggacga cggcatgacg atgaagctcc gggtgcgccg tggcgcttcc cgcgtgggga 2581 acaagatcgt cgttggcaac gccaagaccg tccggtcgaa gcgtcctgtg acggttccgc 2641 ctcacgtcgc ggagatgatc cgagcgcaca tgaaggaccg tacgaagatg aacaagggcc 2701 ccgaggcatt cctggtgacc acgacgcagg gcaaccggct gtcgaagtcc gcgttcacca 2761 agtcgctgaa gcgtggctac gccaagatcg gtcggccgga actccgcatc cacgacctcc 2821 gcgctgtcgg cgctacgttc gccgctcagg caggtgcgac gaccaaggag ctgatggccc 2881 gtctcggtca cacgactcct aggatggcga tgaagtacca gatggcgtct gaggcccgcg 2941 acgaggctat cgctgaggcg atgtccaagc tggccaagac ctcctgaaac gcaaaaagcc 3001 cccctcccaa ggacactgag tcctaaagag gggggtttct tgtcagtacg cgaagaacca 3061 cgcctggccg cgagcgccag caccgccgct ctgtgcggag acctgggcac cagccccgcc 3121 gccgccagga gcattgccgt tcccgccagc tgagttctgt tgtgcgccgc ctatgtagag 3181 ctggtcgttg taggtccgat ctccaggcga ctttccggcg acgctgagga tgtcgatcac 3241 agagcctccg ggaccgccgg ttgcggtcaa acctgaccat ccgacagcgg acgccgtggt 3301 gtttcctcca gggcctccgg ccttgcctga gaatacagag ccagctcccg ctgcgcctcc 3361 agctccgacg agcccggtga tcgtcttggt cgacctgcag gcatgcaagc ttggcgtaat 3421 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 3481 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 3541 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 3601 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 3661 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 3721 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 3781 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 3841 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 3901 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 3961 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 4021 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 4081 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 4141 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 4201 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 4261 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 4321 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 4381 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 4441 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 4501 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 4561 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 4621 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 4681 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 4741 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 4801 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 4861 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 4921 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 4981 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 5041 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 5101 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 5161 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 5221 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 5281 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 5341 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 5401 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 5461 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 5521 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 5581 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 5641 ttcgtc //