pUAStkCAT vector (V002460)

MSDS

Basic Vector Information

Vector Name:: pUAStkCAT

Antibiotic Resistance:: Ampicillin

Length:: 4326 bp

Type:: Mammalian expression vector

Replication origin:: ori

Source/Author:: De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.

Promoter:: Mini-TK

pUAStkCAT vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUAStkCAT vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_44779        4326 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pUAStkCAT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4326)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4326)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    210..227
                     /label=GALbd
                     /note="GALbd"
     misc_feature    228..245
                     /label=GALbd
                     /note="GALbd"
     promoter        340..402
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             470..1126
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1219..1293
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88152.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          1295..1360
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1490..1510
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    1935..2069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2105..2121)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2129..2145)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2153..2183)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2198..2219)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2507..3095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3269..4126)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4127..4231)
                     /label=AmpR promoter

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