LOCUS       40924_44779        4326 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pUAStkCAT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4326)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4326)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    210..227
                     /label=GALbd
                     /note="GALbd"
     misc_feature    228..245
                     /label=GALbd
                     /note="GALbd"
     promoter        340..402
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             470..1126
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1219..1293
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88152.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          1295..1360
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1490..1510
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    1935..2069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2105..2121)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2129..2145)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2153..2183)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2198..2219)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2507..3095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3269..4126)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4127..4231)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataagct tgcatgcctg caggtcgact cggaggacag tactccgctc ggaggacagt
      241 actcccttcg actctagagg atccggcccc gcccagcgtc ttgtcattgg cgaattcgaa
      301 cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta aggtgacgcg
      361 tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca acagcgtgcc
      421 gcagatctcg aggagcttgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa
      481 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      541 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
      601 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
      661 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
      721 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
      781 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
      841 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
      901 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
      961 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     1021 gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat
     1081 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg
     1141 cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa taagtgataa taagcggatg
     1201 aatggcagaa attcgccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     1261 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     1321 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     1381 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     1441 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     1501 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     1561 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     1621 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     1681 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     1741 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     1801 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     1861 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     1921 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1981 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2041 tcatcaatgt atcttatcat gtctggatcg atccccgggt accgagctcg aattcgtaat
     2101 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2161 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2221 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2281 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2341 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2401 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2461 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2521 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2581 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2641 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2701 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2761 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2821 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2881 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2941 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3001 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3061 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3121 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3181 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3241 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3301 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3361 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3421 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3481 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3541 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3601 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3661 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3721 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3781 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3841 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3901 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3961 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4021 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4081 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4141 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4201 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4261 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4321 ttcgtc
//
