Basic Vector Information
pUAST-NTAP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUAST-NTAP vector Sequence
LOCUS 40924_44754 9620 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pUAST-NTAP, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9620)
AUTHORS Veraksa A, Bauer A, Artavanis-Tsakonas S.
TITLE Analyzing protein complexes in Drosophila with tandem affinity
purification-mass spectrometry
JOURNAL Dev. Dyn. 232 (3), 827-834 (2005)
PUBMED 15704125
REFERENCE 2 (bases 1 to 9620)
AUTHORS Veraksa A, Bauer A, Artavanis-Tsakonas S.
TITLE Direct Submission
JOURNAL Submitted (17-AUG-2004) MGH Cancer Center, Harvard Medical School,
Bldg 149, 13th Street, Charlestown, MA 02129, USA
REFERENCE 3 (bases 1 to 9620)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9620)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Dev. Dyn.";
date: "2005"; volume: "232"; issue: "3"; pages: "827-834"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(17-AUG-2004) MGH Cancer Center, Harvard Medical School, Bldg 149,
13th Street, Charlestown, MA 02129, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9620
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(235..823)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(997..1854)
/label=AmpR
/note="beta-lactamase"
promoter complement(1855..1959)
/label=AmpR promoter
misc_feature complement(2761..2993)
/label=P element 3' end
/note="P element 3' end"
protein_bind 3055..3149
/label=5X UAS
/note="five tandem copies of the 'ScaI site' 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
promoter 3168..3406
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
CDS 3461..3634
/label=ProtA
/note="IgG-binding unit of Staphylococcus aureus protein A"
CDS 3638..3808
/codon_start=1
/product="IgG-binding unit of Staphylococcus aureus protein
A"
/label=ProtA
/translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
EAKKLNDAQAPK"
CDS 3845..3865
/label=TEV site
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
CDS 3872..3949
/label=CBP
/note="calmodulin-binding peptide"
CDS 3950..3964
/label=enterokinase site
/note="enterokinase recognition and cleavage site"
misc_feature 3973..4018
/note="polylinker; unique cloning sites for EcoRI,
EagI/NotI, XhoI, KpnI, XbaI"
intron 4096..4161
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4291..4311
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 4736..4870
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
gene 4888..9024
/label=mini-white
/note="This modified version of the white gene lacks part
of the first intron."
misc_feature complement(9035..9620)
/label=P element 5' end
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