LOCUS       40924_44754        9620 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-NTAP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Veraksa A, Bauer A, Artavanis-Tsakonas S.
  TITLE     Analyzing protein complexes in Drosophila with tandem affinity 
            purification-mass spectrometry
  JOURNAL   Dev. Dyn. 232 (3), 827-834 (2005)
  PUBMED    15704125
REFERENCE   2  (bases 1 to 9620)
  AUTHORS   Veraksa A, Bauer A, Artavanis-Tsakonas S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2004) MGH Cancer Center, Harvard Medical School, 
            Bldg 149, 13th Street, Charlestown, MA 02129, USA
REFERENCE   3  (bases 1 to 9620)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9620)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev. Dyn.";
            date: "2005"; volume: "232"; issue: "3"; pages: "827-834"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-AUG-2004) MGH Cancer Center, Harvard Medical School, Bldg 149, 
            13th Street, Charlestown, MA 02129, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(997..1854)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     misc_feature    complement(2761..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3055..3149
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3168..3406
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             3461..3634
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     CDS             3638..3808
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNDAQAPK"
     CDS             3845..3865
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             3872..3949
                     /label=CBP
                     /note="calmodulin-binding peptide"
     CDS             3950..3964
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     misc_feature    3973..4018
                     /note="polylinker; unique cloning sites for EcoRI,
                     EagI/NotI, XhoI, KpnI, XbaI"
     intron          4096..4161
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4291..4311
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4736..4870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            4888..9024
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(9035..9620)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt gcatgcctgc aggtcggagt
     3061 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     3121 gtcctccgag cggagtactg tcctccgagc ggagactcta gcgagcgccg gagtataaat
     3181 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
     3241 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag
     3301 tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac
     3361 tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata gggaattggg
     3421 aattgacacc atggcaggcc ttgcgcaaca cgatgaagcc gtggacaaca aattcaacaa
     3481 agaacaacaa aacgcgttct atgagatctt acatttacct aacttaaacg aagaacaacg
     3541 aaacgccttc atccaaagtt taaaagatga cccaagccaa agcgctaacc ttttagcaga
     3601 agctaaaaag ctaaatgatg ctcaggcgcc gaaagtagac aacaaattca acaaagaaca
     3661 acaaaacgcg ttctatgaga tcttacattt acctaactta aacgaagaac aacgaaacgc
     3721 cttcatccaa agtttaaaag atgacccaag ccaaagcgct aaccttttag cagaagctaa
     3781 aaagctaaat gatgctcagg cgccgaaagt agacgcgaat tgtgatatac ctacaactgc
     3841 ttctgaaaat ttatattttc aaggtgaact aaagagaaga tggaaaaaga atttcatagc
     3901 cgtctcagca gccaaccgct ttaagaaaat ctcatcctcc ggggcacttg atgatgacga
     3961 taagcttatc gggaattcgt taacagatct gcggccgcgg ctcgagggta cctctagagg
     4021 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     4081 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     4141 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     4201 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     4261 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     4321 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     4381 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     4441 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
     4501 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
     4561 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
     4621 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     4681 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     4741 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4801 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4861 tgtctggatc ggatccacta gaaggcctta gtatgtatgt aagttaataa aacccttttt
     4921 tggagaatgt agatttaaaa aaacatattt tttttttatt ttttactgca ctggacatca
     4981 ttgaacttat ctgatcagtt ttaaatttac ttcgatccaa gggtatttga agtaccaggt
     5041 tctttcgatt acctctcact caaaatgaca ttccactcaa agtcagcgct gtttgcctcc
     5101 ttctctgtcc acagaaatat cgccgtctct ttcgccgctg cgtccgctat ctctttcgcc
     5161 accgtttgta gcgttaccta gcgtcaatgt ccgccttcag ttgcactttg tcagcggttt
     5221 cgtgacgaag ctccaagcgg tttacgccat caattaaaca caaagtgctg tgccaaaact
     5281 cctctcgctt cttatttttg tttgtttttt gagtgattgg ggtggtgatt ggttttgggt
     5341 gggtaagcag gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc aggagctatt
     5401 aattcgcgga ggcagcaaac acccatctgc cgagcatctg aacaatgtga gtagtacatg
     5461 tgcatacatc ttaagttcac ttgatctata ggaactgcga ttgcaacatc aaattgtctg
     5521 cggcgtgaga actgcgaccc acaaaaatcc caaaccgcaa tcgcacaaac aaatagtgac
     5581 acgaaacaga ttattctggt agctgtgctc gctatataag acaattttta agatcatatc
     5641 atgatcaaga catctaaagg cattcatttt cgactacatt cttttttaca aaaaatataa
     5701 caaccagata ttttaagctg atcctagatg cacaaaaaat aaataaaagt ataaacctac
     5761 ttcgtaggat acttcgtttt gttcggggtt agatgagcat aacgcttgta gttgatattt
     5821 gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc attaaccagg
     5881 gcttcgggca ggccaaaaac tacggcacgc tcctgccacc cagtccgccg gaggactccg
     5941 gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat atggacatct
     6001 ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg acacgcggac
     6061 tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag aacggtgagt
     6121 ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat taccaatttg
     6181 aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg gcagttccgg
     6241 tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg gcatccaagt
     6301 atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc
     6361 caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg ccagggaaca
     6421 cctgattttc caggccatgg tgcggatgcc acgacatctg acctatcggc agcgagtggc
     6481 ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg
     6541 tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc
     6601 cgaggcacta accgatccgc cgcttctgat ctgcgatgag cccacctccg gactggactc
     6661 atttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt
     6721 catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca agatccttct
     6781 gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg acttcttttc
     6841 ctagtgagtt cgatgtgttt attaagggta tctagcatta cattacatct caactcctat
     6901 ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac gtacaggtgt
     6961 tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag atatgcgaca
     7021 attttgctat tagcaaagta gcccgggata tggagcagtt gttggccacc aaaaatttgg
     7081 agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg ttcatgcagt
     7141 tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag
     7201 tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat aacgcttaca
     7261 attcttggaa acaaattcgc tagattttag ttagaattgc ctgattccac acccttctta
     7321 gtttttttca atgagatgta tagtttatag ttttgcagaa aataaataaa tttcatttaa
     7381 ctcgcgaaca tgttgaagat atgaatatta atgagatgcg agtaacattt taatttgcag
     7441 atggttgcca tcttgattgg cctcatcttt ttgggccaac aactcacgca agtgggcgtg
     7501 atgaatatca acggagccat cttcctcttc ctgaccaaca tgacctttca aaacgtcttt
     7561 gccacgataa atgtaagtct tgtttagaat acatttgcat attaataatt tactaacttt
     7621 ctaatgaatc gattcgattt aggtgttcac ctcagagctg ccagttttta tgagggaggc
     7681 ccgaagtcga ctttatcgct gtgacacata ctttctgggc aaaacgattg ccgaattacc
     7741 gctttttctc acagtgccac tggtcttcac ggcgattgcc tatccgatga tcggactgcg
     7801 ggccggagtg ctgcacttct tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc
     7861 aacgtccttc ggatatctaa tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt
     7921 gggtccgccg gttatcatac cattcctgct ctttggcggc ttcttcttga actcgggctc
     7981 ggtgccagta tacctcaaat ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg
     8041 tctgctgatt aaccaatggg cggacgtgga gccgggcgaa attagctgca catcgtcgaa
     8101 caccacgtgc cccagttcgg gcaaggtcat cctggagacg cttaacttct ccgccgccga
     8161 tctgccgctg gactacgtgg gtctggccat tctcatcgtg agcttccggg tgctcgcata
     8221 tctggctcta agacttcggg cccgacgcaa ggagtagccg acatatatcc gaaataactg
     8281 cttgtttttt tttttaccat tattaccatc gtgtttactg tttattgccc cctcaaaaag
     8341 ctaatgtaat tatatttgtg ccaataaaaa caagatatga cctatagaat acaagtattt
     8401 ccccttcgaa catccccaca agtagacttt ggatttgtct tctaaccaaa agacttacac
     8461 acctgcatac cttacatcaa aaactcgttt atcgctacat aaaacaccgg gatatatttt
     8521 ttatatacat acttttcaaa tcgcgcgccc tcttcataat tcacctccac cacaccacgt
     8581 ttcgtagttg ctctttcgct gtctcccacc cgctctccgc aacacattca ccttttgttc
     8641 gacgaccttg gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa atggttccga
     8701 gtggttcatt tcgtctcaat agaaattagt aataaatatt tgtatgtaca atttatttgc
     8761 tccaatatat ttgtatatat ttccctcaca gctatattta ttctaattta atattatgac
     8821 tttttaaggt aattttttgt gacctgttcg gagtgattag cgttacaatt tgaactgaaa
     8881 gtgacatcca gtgtttgttc cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag
     8941 tgttgtttat atatatcaaa aataacaact ataataataa gaatacattt aatttagaaa
     9001 atgcttggat ttcactggaa ctagaattaa ttcggctgct gctctaaacg acgcatttcg
     9061 tactccaaag tacgaatttt ttccctcaag ctcttatttt cattaaacaa tgaacaggac
     9121 ctaacgcaca gtcacgttat tgtttacata aatgattttt tttactattc aaacttactc
     9181 tgtttgtgta ctcccactgg tatagccttc ttttatcttt tctggttcag gctctatcac
     9241 tttactaggt acggcatctg cgttgagtcg cctcctttta aatgtctgac cttttgcagg
     9301 tgcagccttc cactgcgaat cattaaagtg ggtatcacaa atttgggagt tttcaccaag
     9361 gctgcaccca aggctctgct cccacaattt tctcttaata gcacacttcg gcacgtgaat
     9421 taattttact ccagtcacag ctttgcagca aaatttgcaa tatttcattt ttttttattc
     9481 cacgtaaggg ttaatgtttt caaaaaaaaa ttcgtccgca cacaaccttt cctctcaaca
     9541 agcaaacgtg cactgaattt aagtgtatac ttcggtaagc ttcggctatc gacgggacca
     9601 ccttatgtta tttcatcatg
//
