Basic Vector Information
- Vector Name:
- pUAST-NTAP(GS)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9626 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Kyriakakis P, Tipping M, Abed L, Veraksa A.
- Promoter:
- hsp70
pUAST-NTAP(GS) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUAST-NTAP(GS) vector Sequence
LOCUS 40924_44749 9626 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pUAST-NTAP(GS), complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9626) AUTHORS Kyriakakis P, Tipping M, Abed L, Veraksa A. TITLE Tandem affinity purification in Drosophila: The advantages of the GS-TAP system JOURNAL Fly (Austin) 2 (4) (2008) In press PUBMED 18719405 REFERENCE 2 (bases 1 to 9626) AUTHORS Veraksa A, Kyriakakis P, Tipping M, Abed L. TITLE Direct Submission JOURNAL Submitted (20-APR-2008) Biology Department, University of Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA REFERENCE 3 (bases 1 to 9626) TITLE Direct Submission REFERENCE 4 (bases 1 to 9626) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Fly (Austin) 2 (4) (2008) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (20-APR-2008) Biology Department, University of Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..9626 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(235..823) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(997..1854) /label=AmpR /note="beta-lactamase" promoter complement(1855..1959) /label=AmpR promoter misc_feature complement(2761..2993) /label=P element 3' end /note="P element 3' end" protein_bind 3055..3149 /label=5X UAS /note="five tandem copies of the 'ScaI site' 17-mer CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) that efficiently binds yeast Gal4 (Webster et al., 1988; Pfeiffer et al., 2010)" promoter 3168..3406 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter" CDS 3809..3829 /label=TEV site /note="tobacco etch virus (TEV) protease recognition and cleavage site" CDS 3836..3856 /label=TEV site /note="tobacco etch virus (TEV) protease recognition and cleavage site" CDS 3863..3976 /label=SBP /note="streptavidin-binding peptide" misc_feature 3979..4024 /note="polylinker; unique cloning sites for EcoRI, EagI/NotI, XhoI, KpnI, XbaI" intron 4102..4167 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4297..4317 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" polyA_signal 4742..4876 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" gene 4894..9030 /label=mini-white /note="This modified version of the white gene lacks part of the first intron." misc_feature complement(9041..9626) /label=P element 5' end
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