LOCUS       40924_44749        9626 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-NTAP(GS), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Kyriakakis P, Tipping M, Abed L, Veraksa A.
  TITLE     Tandem affinity purification in Drosophila: The advantages of the 
            GS-TAP system
  JOURNAL   Fly (Austin) 2 (4) (2008) In press
  PUBMED    18719405
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   Veraksa A, Kyriakakis P, Tipping M, Abed L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2008) Biology Department, University of 
            Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA
REFERENCE   3  (bases 1 to 9626)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9626)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) 2 (4) (2008) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-APR-2008) Biology Department, University of Massachusetts 
            Boston, 100 Morrissey Blvd., Boston, MA 02125, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(997..1854)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     misc_feature    complement(2761..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3055..3149
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3168..3406
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             3809..3829
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             3836..3856
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             3863..3976
                     /label=SBP
                     /note="streptavidin-binding peptide"
     misc_feature    3979..4024
                     /note="polylinker; unique cloning sites for EcoRI,
                     EagI/NotI, XhoI, KpnI, XbaI"
     intron          4102..4167
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4297..4317
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4742..4876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            4894..9030
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(9041..9626)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt gcatgcctgc aggtcggagt
     3061 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     3121 gtcctccgag cggagtactg tcctccgagc ggagactcta gcgagcgccg gagtataaat
     3181 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
     3241 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag
     3301 tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac
     3361 tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata gggaattggg
     3421 aattgacacc atgggcaccc ccgcagtcac cacctacaag ctggtcatta acggcaagac
     3481 tctgaagggc gagaccacca ccaaggccgt ggacgcagaa accgcggaaa aggcgttcaa
     3541 gcagtacgcg aacgacaacg gggtggacgg agtctggacc tacgatgacg ctacaaaaac
     3601 gttcaccgtg accgaggtta acacccccgc tgtgactacg tacaagctgg tgatcaatgg
     3661 gaagaccctg aagggcgaga ccaccacgaa agctgtagac gccgagacag ccgagaaggc
     3721 cttcaagcag tacgccaatg acaacggcgt ggatggcgtg tggacctatg acgacgccac
     3781 gaagacattc actgtgactg agatcggcga gaatctgtac tttcagggta tcgatgagaa
     3841 cctctacttc cagggcggca gtatggacga gaagaccacc ggctggagag gcggccacgt
     3901 ggtcgaggga ctggccggtg agctggagca gctacgggcc cgccttgagc accaccccca
     3961 ggggcagcgc gagccgggga attcgttaac agatctgcgg ccgcggctcg agggtacctc
     4021 tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     4081 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     4141 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     4201 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     4261 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     4321 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     4381 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     4441 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     4501 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     4561 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     4621 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     4681 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     4741 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4801 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4861 ttatcatgtc tggatcggat ccactagaag gccttagtat gtatgtaagt taataaaacc
     4921 cttttttgga gaatgtagat ttaaaaaaac atattttttt tttatttttt actgcactgg
     4981 acatcattga acttatctga tcagttttaa atttacttcg atccaagggt atttgaagta
     5041 ccaggttctt tcgattacct ctcactcaaa atgacattcc actcaaagtc agcgctgttt
     5101 gcctccttct ctgtccacag aaatatcgcc gtctctttcg ccgctgcgtc cgctatctct
     5161 ttcgccaccg tttgtagcgt tacctagcgt caatgtccgc cttcagttgc actttgtcag
     5221 cggtttcgtg acgaagctcc aagcggttta cgccatcaat taaacacaaa gtgctgtgcc
     5281 aaaactcctc tcgcttctta tttttgtttg ttttttgagt gattggggtg gtgattggtt
     5341 ttgggtgggt aagcagggga aagtgtgaaa aatcccggca atgggccaag aggatcagga
     5401 gctattaatt cgcggaggca gcaaacaccc atctgccgag catctgaaca atgtgagtag
     5461 tacatgtgca tacatcttaa gttcacttga tctataggaa ctgcgattgc aacatcaaat
     5521 tgtctgcggc gtgagaactg cgacccacaa aaatcccaaa ccgcaatcgc acaaacaaat
     5581 agtgacacga aacagattat tctggtagct gtgctcgcta tataagacaa tttttaagat
     5641 catatcatga tcaagacatc taaaggcatt cattttcgac tacattcttt tttacaaaaa
     5701 atataacaac cagatatttt aagctgatcc tagatgcaca aaaaataaat aaaagtataa
     5761 acctacttcg taggatactt cgttttgttc ggggttagat gagcataacg cttgtagttg
     5821 atatttgaga tcccctatca ttgcagggtg acagcggagc ggcttcgcag agctgcatta
     5881 accagggctt cgggcaggcc aaaaactacg gcacgctcct gccacccagt ccgccggagg
     5941 actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg cacaatatgg
     6001 acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc aaccggacac
     6061 gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg ctcaagaacg
     6121 gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg tccaattacc
     6181 aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg tgatgggcag
     6241 ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat
     6301 ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg ccaaggagat
     6361 gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc taacggccag
     6421 ggaacacctg attttccagg ccatggtgcg gatgccacga catctgacct atcggcagcg
     6481 agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc agcacacgat
     6541 catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt
     6601 cgcctccgag gcactaaccg atccgccgct tctgatctgc gatgagccca cctccggact
     6661 ggactcattt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa
     6721 gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct ttgacaagat
     6781 ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag ccgtcgactt
     6841 cttttcctag tgagttcgat gtgtttatta agggtatcta gcattacatt acatctcaac
     6901 tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac ttttacgtac
     6961 aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc gccaagatat
     7021 gcgacaattt tgctattagc aaagtagccc gggatatgga gcagttgttg gccaccaaaa
     7081 atttggagaa gccactggag cagccggaga atgggtacac ctacaaggcc acctggttca
     7141 tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg
     7201 taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa tgatataacg
     7261 cttacaattc ttggaaacaa attcgctaga ttttagttag aattgcctga ttccacaccc
     7321 ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaaaata aataaatttc
     7381 atttaactcg cgaacatgtt gaagatatga atattaatga gatgcgagta acattttaat
     7441 ttgcagatgg ttgccatctt gattggcctc atctttttgg gccaacaact cacgcaagtg
     7501 ggcgtgatga atatcaacgg agccatcttc ctcttcctga ccaacatgac ctttcaaaac
     7561 gtctttgcca cgataaatgt aagtcttgtt tagaatacat ttgcatatta ataatttact
     7621 aactttctaa tgaatcgatt cgatttaggt gttcacctca gagctgccag tttttatgag
     7681 ggaggcccga agtcgacttt atcgctgtga cacatacttt ctgggcaaaa cgattgccga
     7741 attaccgctt tttctcacag tgccactggt cttcacggcg attgcctatc cgatgatcgg
     7801 actgcgggcc ggagtgctgc acttcttcaa ctgcctggcg ctggtcactc tggtggccaa
     7861 tgtgtcaacg tccttcggat atctaatatc ctgcgccagc tcctcgacct cgatggcgct
     7921 gtctgtgggt ccgccggtta tcataccatt cctgctcttt ggcggcttct tcttgaactc
     7981 gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa
     8041 cgagggtctg ctgattaacc aatgggcgga cgtggagccg ggcgaaatta gctgcacatc
     8101 gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg gagacgctta acttctccgc
     8161 cgccgatctg ccgctggact acgtgggtct ggccattctc atcgtgagct tccgggtgct
     8221 cgcatatctg gctctaagac ttcgggcccg acgcaaggag tagccgacat atatccgaaa
     8281 taactgcttg tttttttttt taccattatt accatcgtgt ttactgttta ttgccccctc
     8341 aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta tagaatacaa
     8401 gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta accaaaagac
     8461 ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa caccgggata
     8521 tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac ctccaccaca
     8581 ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca cattcacctt
     8641 ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc gctcaaatgg
     8701 ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta tgtacaattt
     8761 atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct aatttaatat
     8821 tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt acaatttgaa
     8881 ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa atggtgggca
     8941 taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat acatttaatt
     9001 tagaaaatgc ttggatttca ctggaactag aattaattcg gctgctgctc taaacgacgc
     9061 atttcgtact ccaaagtacg aattttttcc ctcaagctct tattttcatt aaacaatgaa
     9121 caggacctaa cgcacagtca cgttattgtt tacataaatg atttttttta ctattcaaac
     9181 ttactctgtt tgtgtactcc cactggtata gccttctttt atcttttctg gttcaggctc
     9241 tatcacttta ctaggtacgg catctgcgtt gagtcgcctc cttttaaatg tctgaccttt
     9301 tgcaggtgca gccttccact gcgaatcatt aaagtgggta tcacaaattt gggagttttc
     9361 accaaggctg cacccaaggc tctgctccca caattttctc ttaatagcac acttcggcac
     9421 gtgaattaat tttactccag tcacagcttt gcagcaaaat ttgcaatatt tcattttttt
     9481 ttattccacg taagggttaa tgttttcaaa aaaaaattcg tccgcacaca acctttcctc
     9541 tcaacaagca aacgtgcact gaatttaagt gtatacttcg gtaagcttcg gctatcgacg
     9601 ggaccacctt atgttatttc atcatg
//
