Basic Vector Information
pUAST-CTAP(SG) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUAST-CTAP(SG) vector Sequence
LOCUS 40924_44739 9621 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pUAST-CTAP(SG), complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9621)
AUTHORS Kyriakakis P, Tipping M, Abed L, Veraksa A.
TITLE Tandem affinity purification in Drosophila: The advantages of the
GS-TAP system
JOURNAL Fly (Austin) 2 (4) (2008) In press
PUBMED 18719405
REFERENCE 2 (bases 1 to 9621)
AUTHORS Veraksa A, Kyriakakis P, Tipping M, Abed L.
TITLE Direct Submission
JOURNAL Submitted (20-APR-2008) Biology Department, University of
Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA
REFERENCE 3 (bases 1 to 9621)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9621)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Fly
(Austin) 2 (4) (2008) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(20-APR-2008) Biology Department, University of Massachusetts
Boston, 100 Morrissey Blvd., Boston, MA 02125, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9621
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(235..823)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(997..1854)
/label=AmpR
/note="beta-lactamase"
promoter complement(1855..1959)
/label=AmpR promoter
misc_feature complement(2761..2993)
/label=P element 3' end
/note="P element 3' end"
protein_bind 3055..3149
/label=5X UAS
/note="five tandem copies of the 'ScaI site' 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
promoter 3168..3406
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
misc_feature 3420..3465
/note="polylinker; unique cloning sites for EcoRI, NotI
(EagI cannot be used), XhoI, KpnI, XbaI"
CDS 3469..3582
/label=SBP
/note="streptavidin-binding peptide"
CDS 3589..3609
/label=TEV site
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
CDS 3616..3636
/label=TEV site
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
intron 4097..4162
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4292..4312
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 4737..4871
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
gene 4889..9025
/label=mini-white
/note="This modified version of the white gene lacks part
of the first intron."
misc_feature complement(9036..9621)
/label=P element 5' end
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