LOCUS       40924_44739        9621 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-CTAP(SG), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Kyriakakis P, Tipping M, Abed L, Veraksa A.
  TITLE     Tandem affinity purification in Drosophila: The advantages of the 
            GS-TAP system
  JOURNAL   Fly (Austin) 2 (4) (2008) In press
  PUBMED    18719405
REFERENCE   2  (bases 1 to 9621)
  AUTHORS   Veraksa A, Kyriakakis P, Tipping M, Abed L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2008) Biology Department, University of 
            Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA
REFERENCE   3  (bases 1 to 9621)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9621)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) 2 (4) (2008) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-APR-2008) Biology Department, University of Massachusetts 
            Boston, 100 Morrissey Blvd., Boston, MA 02125, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(997..1854)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     misc_feature    complement(2761..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3055..3149
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3168..3406
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    3420..3465
                     /note="polylinker; unique cloning sites for EcoRI, NotI
                     (EagI cannot be used), XhoI, KpnI, XbaI"
     CDS             3469..3582
                     /label=SBP
                     /note="streptavidin-binding peptide"
     CDS             3589..3609
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             3616..3636
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     intron          4097..4162
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4292..4312
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4737..4871
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            4889..9025
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(9036..9621)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt gcatgcctgc aggtcggagt
     3061 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     3121 gtcctccgag cggagtactg tcctccgagc ggagactcta gcgagcgccg gagtataaat
     3181 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
     3241 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag
     3301 tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac
     3361 tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata gggaattggg
     3421 aattcgttaa cagatctgcg gccgcggctc gagggtacct ctagaggcat ggacgagaag
     3481 accaccgggt ggcggggcgg ccacgttgtg gagggtctcg ctggcgagct ggagcagctc
     3541 agggcccgct tggagcacca tccccagggg caacgcgagc ctatcgatga aaatctgtac
     3601 ttccagggga tcgatgagaa cctgtacttt caggggggta gtaccccggc cgtgacgacc
     3661 tacaagctgg tgatcaacgg caagaccctg aagggcgaga ccacaaccaa ggccgtggac
     3721 gctgaaaccg cagagaaggc cttcaagcag tacgccaacg acaatggcgt ggacggagtg
     3781 tggacctacg acgatgccac caagaccttc accgtgaccg aggttaacac ccccgcagtc
     3841 accacataca aactggtgat caacggaaaa acgctgaagg gcgaaactac gaccaaggct
     3901 gtggacgcgg agacggccga aaaggcgttc aaacagtatg ccaacgataa cggtgtagac
     3961 ggcgtctgga catatgacga cgcgacaaag actttcactg tcactgaata atgactagag
     4021 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
     4081 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
     4141 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
     4201 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
     4261 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
     4321 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
     4381 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
     4441 tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc ataacatact
     4501 gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt
     4561 gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc
     4621 cttgactaga gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     4681 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     4741 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4801 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4861 atgtctggat cggatccact agaaggcctt agtatgtatg taagttaata aaaccctttt
     4921 ttggagaatg tagatttaaa aaaacatatt ttttttttat tttttactgc actggacatc
     4981 attgaactta tctgatcagt tttaaattta cttcgatcca agggtatttg aagtaccagg
     5041 ttctttcgat tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc
     5101 cttctctgtc cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc
     5161 caccgtttgt agcgttacct agcgtcaatg tccgccttca gttgcacttt gtcagcggtt
     5221 tcgtgacgaa gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac
     5281 tcctctcgct tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg
     5341 tgggtaagca ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat
     5401 taattcgcgg aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat
     5461 gtgcatacat cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct
     5521 gcggcgtgag aactgcgacc cacaaaaatc ccaaaccgca atcgcacaaa caaatagtga
     5581 cacgaaacag attattctgg tagctgtgct cgctatataa gacaattttt aagatcatat
     5641 catgatcaag acatctaaag gcattcattt tcgactacat tcttttttac aaaaaatata
     5701 acaaccagat attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta
     5761 cttcgtagga tacttcgttt tgttcggggt tagatgagca taacgcttgt agttgatatt
     5821 tgagatcccc tatcattgca gggtgacagc ggagcggctt cgcagagctg cattaaccag
     5881 ggcttcgggc aggccaaaaa ctacggcacg ctcctgccac ccagtccgcc ggaggactcc
     5941 ggttcaggga gcggccaact agccgagaac ctcacctatg cctggcacaa tatggacatc
     6001 tttggggcgg tcaatcagcc gggctccgga tggcggcagc tggtcaaccg gacacgcgga
     6061 ctattctgca acgagcgaca cataccggcg cccaggaaac atttgctcaa gaacggtgag
     6121 tttctattcg cagtcggctg atctgtgtga aatcttaata aagggtccaa ttaccaattt
     6181 gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt ggccgtgatg ggcagttccg
     6241 gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg atcgccgcag ggcatccaag
     6301 tatcgccatc cgggatgcga ctgctcaatg gccaacctgt ggacgccaag gagatgcagg
     6361 ccaggtgcgc ctatgtccag caggatgacc tctttatcgg ctccctaacg gccagggaac
     6421 acctgatttt ccaggccatg gtgcggatgc cacgacatct gacctatcgg cagcgagtgg
     6481 cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa atgtcagcac acgatcatcg
     6541 gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag gaagcgtctg gcattcgcct
     6601 ccgaggcact aaccgatccg ccgcttctga tctgcgatga gcccacctcc ggactggact
     6661 catttaccgc ccacagcgtc gtccaggtgc tgaagaagct gtcgcagaag ggcaagaccg
     6721 tcatcctgac cattcatcag ccgtcttccg agctgtttga gctctttgac aagatccttc
     6781 tgatggccga gggcagggta gctttcttgg gcactcccag cgaagccgtc gacttctttt
     6841 cctagtgagt tcgatgtgtt tattaagggt atctagcatt acattacatc tcaactccta
     6901 tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg cggactttta cgtacaggtg
     6961 ttggccgttg tgcccggacg ggagatcgag tcccgtgatc ggatcgccaa gatatgcgac
     7021 aattttgcta ttagcaaagt agcccgggat atggagcagt tgttggccac caaaaatttg
     7081 gagaagccac tggagcagcc ggagaatggg tacacctaca aggccacctg gttcatgcag
     7141 ttccgggcgg tcctgtggcg atcctggctg tcggtgctca aggaaccact cctcgtaaaa
     7201 gtgcgactta ttcagacaac ggtgagtggt tccagtggaa acaaatgata taacgcttac
     7261 aattcttgga aacaaattcg ctagatttta gttagaattg cctgattcca cacccttctt
     7321 agtttttttc aatgagatgt atagtttata gttttgcaga aaataaataa atttcattta
     7381 actcgcgaac atgttgaaga tatgaatatt aatgagatgc gagtaacatt ttaatttgca
     7441 gatggttgcc atcttgattg gcctcatctt tttgggccaa caactcacgc aagtgggcgt
     7501 gatgaatatc aacggagcca tcttcctctt cctgaccaac atgacctttc aaaacgtctt
     7561 tgccacgata aatgtaagtc ttgtttagaa tacatttgca tattaataat ttactaactt
     7621 tctaatgaat cgattcgatt taggtgttca cctcagagct gccagttttt atgagggagg
     7681 cccgaagtcg actttatcgc tgtgacacat actttctggg caaaacgatt gccgaattac
     7741 cgctttttct cacagtgcca ctggtcttca cggcgattgc ctatccgatg atcggactgc
     7801 gggccggagt gctgcacttc ttcaactgcc tggcgctggt cactctggtg gccaatgtgt
     7861 caacgtcctt cggatatcta atatcctgcg ccagctcctc gacctcgatg gcgctgtctg
     7921 tgggtccgcc ggttatcata ccattcctgc tctttggcgg cttcttcttg aactcgggct
     7981 cggtgccagt atacctcaaa tggttgtcgt acctctcatg gttccgttac gccaacgagg
     8041 gtctgctgat taaccaatgg gcggacgtgg agccgggcga aattagctgc acatcgtcga
     8101 acaccacgtg ccccagttcg ggcaaggtca tcctggagac gcttaacttc tccgccgccg
     8161 atctgccgct ggactacgtg ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat
     8221 atctggctct aagacttcgg gcccgacgca aggagtagcc gacatatatc cgaaataact
     8281 gcttgttttt ttttttacca ttattaccat cgtgtttact gtttattgcc ccctcaaaaa
     8341 gctaatgtaa ttatatttgt gccaataaaa acaagatatg acctatagaa tacaagtatt
     8401 tccccttcga acatccccac aagtagactt tggatttgtc ttctaaccaa aagacttaca
     8461 cacctgcata ccttacatca aaaactcgtt tatcgctaca taaaacaccg ggatatattt
     8521 tttatataca tacttttcaa atcgcgcgcc ctcttcataa ttcacctcca ccacaccacg
     8581 tttcgtagtt gctctttcgc tgtctcccac ccgctctccg caacacattc accttttgtt
     8641 cgacgacctt ggagcgactg tcgttagttc cgcgcgattc ggttcgctca aatggttccg
     8701 agtggttcat ttcgtctcaa tagaaattag taataaatat ttgtatgtac aatttatttg
     8761 ctccaatata tttgtatata tttccctcac agctatattt attctaattt aatattatga
     8821 ctttttaagg taattttttg tgacctgttc ggagtgatta gcgttacaat ttgaactgaa
     8881 agtgacatcc agtgtttgtt ccttgtgtag atgcatctca aaaaaatggt gggcataata
     8941 gtgttgttta tatatatcaa aaataacaac tataataata agaatacatt taatttagaa
     9001 aatgcttgga tttcactgga actagaatta attcggctgc tgctctaaac gacgcatttc
     9061 gtactccaaa gtacgaattt tttccctcaa gctcttattt tcattaaaca atgaacagga
     9121 cctaacgcac agtcacgtta ttgtttacat aaatgatttt ttttactatt caaacttact
     9181 ctgtttgtgt actcccactg gtatagcctt cttttatctt ttctggttca ggctctatca
     9241 ctttactagg tacggcatct gcgttgagtc gcctcctttt aaatgtctga ccttttgcag
     9301 gtgcagcctt ccactgcgaa tcattaaagt gggtatcaca aatttgggag ttttcaccaa
     9361 ggctgcaccc aaggctctgc tcccacaatt ttctcttaat agcacacttc ggcacgtgaa
     9421 ttaattttac tccagtcaca gctttgcagc aaaatttgca atatttcatt tttttttatt
     9481 ccacgtaagg gttaatgttt tcaaaaaaaa attcgtccgc acacaacctt tcctctcaac
     9541 aagcaaacgt gcactgaatt taagtgtata cttcggtaag cttcggctat cgacgggacc
     9601 accttatgtt atttcatcat g
//
