Basic Vector Information
pUAST-C5 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUAST-C5 vector Sequence
LOCUS 40924_44734 9110 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning/transgenesis vector pUAST-C5, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9110)
AUTHORS Le T, Yu M, Williams B, Goel S, Paul SM, Beitel GJ.
TITLE CaSpeR5, a family of Drosophila transgenesis and shuttle vectors
with improved multiple cloning sites
JOURNAL BioTechniques 42 (2), 164 (2007)
PUBMED 17373479
REFERENCE 2 (bases 1 to 9110)
AUTHORS Beitel GJ.
TITLE Direct Submission
JOURNAL Submitted (27-OCT-2006) BMBCB, Northwestern University, 2205 Tech
Dr., Evanston, IL 60091, USA
REFERENCE 3 (bases 1 to 9110)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9110)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"BioTechniques"; date: "2007"; volume: "42"; issue: "2"; pages:
"164"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(27-OCT-2006) BMBCB, Northwestern University, 2205 Tech Dr.,
Evanston, IL 60091, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9110
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(235..823)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(997..1854)
/label=AmpR
/note="beta-lactamase"
promoter complement(1855..1959)
/label=AmpR promoter
misc_feature complement(2761..2993)
/label=P element 3' end
/note="P element 3' end"
protein_bind 3055..3149
/label=5X UAS
/note="five tandem copies of the 'ScaI site' 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
promoter 3168..3406
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
misc_feature 3426..3649
/note="ppCasper5 multiple cloning site but with mutated
SphI site to eliminate ATG in the multiple cloning site"
intron 3738..3803
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 3933..3953
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 4225..4359
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
gene 4377..8514
/label=mini-white
/note="This modified version of the white gene lacks part
of the first intron."
misc_feature complement(8525..9110)
/label=P element 5' end
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