LOCUS       40924_44734        9110 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning/transgenesis vector pUAST-C5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Le T, Yu M, Williams B, Goel S, Paul SM, Beitel GJ.
  TITLE     CaSpeR5, a family of Drosophila transgenesis and shuttle vectors 
            with improved multiple cloning sites
  JOURNAL   BioTechniques 42 (2), 164 (2007)
  PUBMED    17373479
REFERENCE   2  (bases 1 to 9110)
  AUTHORS   Beitel GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) BMBCB, Northwestern University, 2205 Tech 
            Dr., Evanston, IL 60091, USA
REFERENCE   3  (bases 1 to 9110)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9110)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2007"; volume: "42"; issue: "2"; pages: 
            "164"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-2006) BMBCB, Northwestern University, 2205 Tech Dr., 
            Evanston, IL 60091, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(997..1854)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     misc_feature    complement(2761..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3055..3149
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3168..3406
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    3426..3649
                     /note="ppCasper5 multiple cloning site but with mutated
                     SphI site to eliminate ATG in the multiple cloning site"
     intron          3738..3803
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3933..3953
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4225..4359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            4377..8514
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(8525..9110)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt gcatgcctgc aggtcggagt
     3061 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     3121 gtcctccgag cggagtactg tcctccgagc ggagactcta gcgagcgccg gagtataaat
     3181 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
     3241 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag
     3301 tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac
     3361 tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata gggaattggg
     3421 aattggccgg ccaattaatt aagtggaatt caaaggtacc cgcccgggat cagatccgcg
     3481 gccgcatagg ccactagtgg atctggatcc ttcttaagaa gagctctaaa gctttatcta
     3541 gagtcgacct cgaacgttaa ctcgaggcct ataagatctc ttgcatacta cctgcaggat
     3601 accggtataa cgcgtatagc tagctttcct aggtctgcga tcgcaaaggc gcgccctaga
     3661 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     3721 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     3781 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     3841 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     3901 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     3961 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     4021 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     4081 ttatggaaaa atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     4141 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     4201 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     4261 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4321 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc actagaaggc ctaattctag
     4381 tatgtatgta agttaataaa acccattttt tggagaatgt agatttaaaa aaacatattt
     4441 tttttttatt ttttactgca ctggacatca ttgaacttat ctgatcagtt ttaaatttac
     4501 ttcgatccaa gggtatttga agtaccaggt tctttcgatt acctctcact caaaatgaca
     4561 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
     4621 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttaccta gcgtcaatgt
     4681 ccgccttcag ttgcactttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     4741 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     4801 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     4861 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     4921 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     4981 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     5041 caaaccgcaa tcgcacaaac aaatagtgac acgaaacaga ttattctggt agctgtgctc
     5101 gctatataag acaattttta agatcatatc atgatcaaga catctaaagg cattcatttt
     5161 cgactacatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     5221 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcgtttt gttcggggtt
     5281 agatgagcat aacgcttgta gttgatattt gagatcccct atcattgcag ggtgacagcg
     5341 gagcggcttc gcagagctgc attaaccagg gcttcgggca ggccaaaaac tacggcacgc
     5401 tcctgccacc cagtccgccg gaggactccg gttcagggag cggccaacta gccgagaacc
     5461 tcacctatgc ctggcacaat atggacatct ttggggcggt caatcagccg ggctccggat
     5521 ggcggcagct ggtcaaccgg acacgcggac tattctgcaa cgagcgacac ataccggcgc
     5581 ccaggaaaca tttgctcaag aacggtgagt ttctattcgc agtcggctga tctgtgtgaa
     5641 atcttaataa agggtccaat taccaatttg aaactcagtt tgcggcgtgg cctatccggg
     5701 cgaacttttg gccgtgatgg gcagttccgg tgccggaaag acgaccctgc tgaatgccct
     5761 tgcctttcga tcgccgcagg gcatccaagt atcgccatcc gggatgcgac tgctcaatgg
     5821 ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc tatgtccagc aggatgacct
     5881 ctttatcggc tccctaacgg ccagggaaca cctgattttc caggccatgg tgcggatgcc
     5941 acgacatctg acctatcggc agcgagtggc ccgcgtggat caggtgatcc aggagctttc
     6001 gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg
     6061 cggagaaagg aagcgtctgg cattcgcctc cgaggcacta accgatccgc cgcttctgat
     6121 ctgcgatgag cccacctccg gactggactc atttaccgcc cacagcgtcg tccaggtgct
     6181 gaagaagctg tcgcagaagg gcaagaccgt catcctgacc attcatcagc cgtcttccga
     6241 gctgtttgag ctctttgaca agatccttct gatggccgag ggcagggtag ctttcttggg
     6301 cactcccagc gaagccgtcg acttcttttc ctagtgagtt cgatgtgttt attaagggta
     6361 tctagcatta cattacatct caactcctat ccagcgtggg tgcccagtgt cctaccaact
     6421 acaatccggc ggacttttac gtacaggtgt tggccgttgt gcccggacgg gagatcgagt
     6481 cccgtgatcg gatcgccaag atatgcgaca attttgctat tagcaaagta gcccgggata
     6541 tggagcagtt gttggccacc aaaaatttgg agaagccact ggagcagccg gagaatgggt
     6601 acacctacaa ggccacctgg ttcatgcagt tccgggcggt cctgtggcga tcctggctgt
     6661 cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt
     6721 ccagtggaaa caaatgatat aacgcttaca attcttggaa acaaattcgc tagattttag
     6781 ttagaattgc ctgattccac acccttctta gtttttttca atgagatgta tagtttatag
     6841 ttttgcagaa aataaataaa tttcatttaa ctcgcgaaca tgttgaagat atgaatatta
     6901 atgagatgcg agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt
     6961 ttgggccaac aactcacgca agtgggcgtg atgaatatca acggagccat cttcctcttc
     7021 ctgaccaaca tgacctttca aaacgtcttt gccacgataa atgtaagtct tgtttagaat
     7081 acatttgcat attaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac
     7141 ctcagagctg ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata
     7201 ctttctgggc aaaacgattg ccgaattacc gctttttctc acagtgccac tggtcttcac
     7261 ggcgattgcc tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct
     7321 ggcgctggtc actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc
     7381 cagctcctcg acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct
     7441 ctttggcggc ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta
     7501 cctctcatgg ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga
     7561 gccgggcgaa attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat
     7621 cctggagacg cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat
     7681 tctcatcgtg agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa
     7741 ggagtagccg acatatatcc gaaataactg cttgtttttt tttttaccat tattaccatc
     7801 gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa
     7861 caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca agtagacttt
     7921 ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa aaactcgttt
     7981 atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa tcgcgcgccc
     8041 tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct gtctcccacc
     8101 cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt cgttagttcc
     8161 gcgcgattcg gttcgctcaa atggttccga gtggttcatt tcgtctcaat agaaattagt
     8221 aataaatatt tgtatgtaca atttatttgc tccaatatat ttgtatatat ttccctcaca
     8281 gctatattta ttctaattta atattatgac tttttaaggt aattttttgt gacctgttcg
     8341 gagtgattag cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga
     8401 tgcatctcaa aaaaatggtg ggcataatag tgttgtttat atatatcaaa aataacaact
     8461 ataataataa gaatacattt aatttagaaa atgcttggat ttcactggaa ctagaattaa
     8521 ttcggctgct gctctaaacg acgcatttcg tactccaaag tacgaatttt ttccctcaag
     8581 ctcttatttt cattaaacaa tgaacaggac ctaacgcaca gtcacgttat tgtttacata
     8641 aatgattttt tttactattc aaacttactc tgtttgtgta ctcccactgg tatagccttc
     8701 ttttatcttt tctggttcag gctctatcac tttactaggt acggcatctg cgttgagtcg
     8761 cctcctttta aatgtctgac cttttgcagg tgcagccttc cactgcgaat cattaaagtg
     8821 ggtatcacaa atttgggagt tttcaccaag gctgcaccca aggctctgct cccacaattt
     8881 tctcttaata gcacacttcg gcacgtgaat taattttact ccagtcacag ctttgcagca
     8941 aaatttgcaa tatttcattt ttttttattc cacgtaaggg ttaatgtttt caaaaaaaaa
     9001 ttcgtccgca cacaaccttt cctctcaaca agcaaacgtg cactgaattt aagtgtatac
     9061 ttcggtaagc ttcggctatc gacgggacca ccttatgtta tttcatcatg
//
