Basic Vector Information
pUASpDir vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUASpDir vector Sequence
LOCUS 40924_44704 10052 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pUASpDir, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10052) AUTHORS Jenny A, Mlodzik M. TITLE Modified vectors for the two-step directional cloning of inverted repeats for RNA interference in Drosophila JOURNAL BioTechniques 44 (3), 335-339 (2008) PUBMED 18361787 REFERENCE 2 (bases 1 to 10052) AUTHORS Jenny A, Mlodzik M. TITLE Direct Submission JOURNAL Submitted (26-SEP-2007) Brookdale Department of Molecular, Cell and Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L. Levi Place Annenberg 18-92 Box 1020, New York, NY 10029, USA REFERENCE 3 (bases 1 to 10052) TITLE Direct Submission REFERENCE 4 (bases 1 to 10052) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "BioTechniques"; date: "2008"; volume: "44"; issue: "3"; pages: "335-339" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (26-SEP-2007) Brookdale Department of Molecular, Cell and Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L. Levi Place Annenberg 18-92 Box 1020, New York, NY 10029, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..10052 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1750..1982 /label=P element 3' end /note="P element 3' end" promoter 2784..2888 /label=AmpR promoter CDS 2889..3746 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3920..4508 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 4743..5328 /label=P element 5' end gene complement(5339..9474) /label=mini-white /note="This modified version of the white gene lacks part of the first intron."
This page is informational only.