pUASpDir vector (V002474)

LOCUS       40924_44704       10052 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUASpDir, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Jenny A, Mlodzik M.
  TITLE     Modified vectors for the two-step directional cloning of inverted 
            repeats for RNA interference in Drosophila
  JOURNAL   BioTechniques 44 (3), 335-339 (2008)
  PUBMED    18361787
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   Jenny A, Mlodzik M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2007) Brookdale Department of Molecular, Cell and 
            Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L. 
            Levi Place Annenberg 18-92 Box 1020, New York, NY 10029, USA
REFERENCE   3  (bases 1 to 10052)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10052)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2008"; volume: "44"; issue: "3"; pages: 
            "335-339"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2007) Brookdale Department of Molecular, Cell and 
            Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L. 
            Levi Place Annenberg 18-92 Box 1020, New York, NY 10029, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1750..1982
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        2784..2888
                     /label=AmpR promoter
     CDS             2889..3746
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3920..4508
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4743..5328
                     /label=P element 5' end
     gene            complement(5339..9474)
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."