pUASpDir vector (V002474)

Basic Vector Information

      • Vector Name:
      • pUASpDir
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 10052 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Jenny A, Mlodzik M.

pUASpDir vector Vector Map

pUASpDir10052 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000P element 3' endAmpR promoterAmpRoriP element 5' endmini-white

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUASpDir vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_44704       10052 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUASpDir, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Jenny A, Mlodzik M.
  TITLE     Modified vectors for the two-step directional cloning of inverted 
            repeats for RNA interference in Drosophila
  JOURNAL   BioTechniques 44 (3), 335-339 (2008)
  PUBMED    18361787
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   Jenny A, Mlodzik M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2007) Brookdale Department of Molecular, Cell and 
            Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L. 
            Levi Place Annenberg 18-92 Box 1020, New York, NY 10029, USA
REFERENCE   3  (bases 1 to 10052)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10052)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2008"; volume: "44"; issue: "3"; pages: 
            "335-339"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2007) Brookdale Department of Molecular, Cell and 
            Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L. 
            Levi Place Annenberg 18-92 Box 1020, New York, NY 10029, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1750..1982
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        2784..2888
                     /label=AmpR promoter
     CDS             2889..3746
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3920..4508
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4743..5328
                     /label=P element 5' end
     gene            complement(5339..9474)
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."

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