LOCUS       40924_44704       10052 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUASpDir, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Jenny A, Mlodzik M.
  TITLE     Modified vectors for the two-step directional cloning of inverted 
            repeats for RNA interference in Drosophila
  JOURNAL   BioTechniques 44 (3), 335-339 (2008)
  PUBMED    18361787
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   Jenny A, Mlodzik M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2007) Brookdale Department of Molecular, Cell and 
            Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L. 
            Levi Place Annenberg 18-92 Box 1020, New York, NY 10029, USA
REFERENCE   3  (bases 1 to 10052)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10052)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2008"; volume: "44"; issue: "3"; pages: 
            "335-339"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2007) Brookdale Department of Molecular, Cell and 
            Developmental Biology, Mount Sinai School of Medicine, 1 Gustave L. 
            Levi Place Annenberg 18-92 Box 1020, New York, NY 10029, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1750..1982
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        2784..2888
                     /label=AmpR promoter
     CDS             2889..3746
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3920..4508
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4743..5328
                     /label=P element 5' end
     gene            complement(5339..9474)
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
ORIGIN
        1 ccggtgatag agcctgaacc agaaaagata aaagaaggct ataccagtgg gagtacacaa
       61 acagagtaag tttgaatagt aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt
      121 taggtcctgt tcattggtac cgaattcgtt aacagatctg cggccgcggc tcgagactag
      181 tgcctaggtg agtttctatt cgcagtcggc tgatctgtgt gaaatcttaa tgaagggtcc
      241 aattaccaat ttgaaactca gctagctcta gaatatcaat tgggatcccc cctagagtcg
      301 acctcgaacg ttaacgttaa cgtaacgtta actcgaggag cttgataaca ttatacctaa
      361 acccatggtc aagagtaaac atttctgcct ttgaagttga gaacacaatt aagcatcccc
      421 tggttaaacc tgacacttca tacttgttaa tagcgccata aacatagcac caatttcgaa
      481 gaaatcagtt aaaagcaatt agcaattagc aattagcaat aactctgctg acttcaaaac
      541 gagaagagtt gcaagtattt gtaaggcaca gtttatagac caccgacggc tcattagggc
      601 tcgtcatgta actaagcgcg gtgaaaccca attgaacagt agtaggtgga attagttagt
      661 tagtcaatgg ggaagattta accctcaggt agcaaagtaa ttgaactgcg ggcagagagt
      721 cctaagacta aataatatat ttaaaaatct ggccctttga ccttgcttgt caggtgcatt
      781 tgggttcaat cgtaagttgc ttctatataa acactttccc catccccgca ataatgaaga
      841 ataccgcaga ataaagagag atttgcaaca aaaaataaag gcattgcgaa aactttttat
      901 gggggatcat tacactcggg cctacggtta caattcccag ccacttaagc gacaagtttg
      961 gccaacaatc catctaatag ctaatagcgc aatcactggt aatcgcaaga gtatataggg
     1021 caatagaacc catggatttg accaaaggta accgagacaa tggagaagca agaggatttc
     1081 aaactgaaca cccacagtgc tgtgtactac cactggcgcg tttggagctc actggcctga
     1141 tgcgctcctc cgggcgtttc aagcctgctt tacgtggtat actccattac ggtcaagttg
     1201 gtggtcaccg tgctgtttcc cttgagcttg ctggccaggc tgctgttcac caccaacatg
     1261 gccggattgt gcgagaacct gaccataagt attaccgata ttgtggccaa tttgaagttt
     1321 gcgaatgtgt acatggtgag gaagcagctc catgagattc gctctctcct aaggctcatg
     1381 gacgctagag cccggctggt gggcgatccc gaggagattt ctgccttgag gaaggaagtg
     1441 aatatcggca cagggcactt tccagcacct ttggccagta ttttcgtatt tggcactact
     1501 ttgagttcgc gtccgcgtgg tcgttcgccc ggatcgagag ctcctgtatc cggcctggtt
     1561 cggcgttgac tggattcact ccaccagaaa ctatgtgctc atcaatatct accagctctt
     1621 cggcttgata gtgcaggcta tacagaactg cgctagtgac tcctatccgc ctgcgtttct
     1681 ctgcctgctc acgggtcata tgcgtgcttt ggagctgagg gtgcggcgga ttggctgcag
     1741 cccaagcttt gcgtactcgc aaattattaa aaataaaact ttaaaaataa tttcgtctaa
     1801 ttaatattat gagttaattc aaaccccacg gacatgctaa gggttaatca acaatcatat
     1861 cgctgtctca ctcagactca atacgacact cagaatacta ttcctttcac tcgcacttat
     1921 tgcaagcata cgttaagtgg atgtctcttg ccgacgggac caccttatgt tatttcatca
     1981 tggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg
     2041 ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt tttttttacc
     2101 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     2161 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     2221 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     2281 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     2341 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     2401 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     2461 gtcgttagtt ccgcgcgatt cggtgcggta tttcacaccg catatggtgc actctcagta
     2521 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     2581 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     2641 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     2701 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     2761 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     2821 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2881 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     2941 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3001 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3061 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3121 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     3181 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     3241 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     3301 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     3361 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     3421 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     3481 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     3541 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     3601 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     3661 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     3721 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     3781 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     3841 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     3901 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3961 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4021 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     4081 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4141 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4201 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4261 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
     4321 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4381 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4441 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4501 tatggaaaaa cgccttcttc ttgaactcgg gctcggtgcc agtatacctc aaatggttgt
     4561 cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa tgggcggacg
     4621 tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg
     4681 tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac gtgggtctgg
     4741 cccatgatga aataacataa ggtggtcccg tcgatagccg aagcttaccg aagtatacac
     4801 ttaaattcag tgcacgtttg cttgttgaga ggaaaggttg tgtgcggacg aatttttttt
     4861 tgaaaacatt aacccttacg tggaataaaa aaaaatgaaa tattgcaaat tttgctgcaa
     4921 agctgtgact ggagtaaaat taattcacgt gccgaagtgt gctattaaga gaaaattgtg
     4981 ggagcagagc cttgggtgca gccttggtga aaactcccaa atttgtgata cccactttaa
     5041 agattcgcag tggaaggctg cacctgcaaa aggtcagaca tttaaaagga ggcgactcaa
     5101 cgcagatgcc gtacctagta aagtgataga gcctgaacca gaaaagataa aagaaggcta
     5161 taccagtggg agtacacaaa cagagtaagt ttgaatagta aaaaaaatca tttatgtaaa
     5221 caataacgtg actgtgcgtt aggtcctgtt cattgtttaa tgaaaataag agcttgaggg
     5281 aaaaaattcg tactttggag tacgaaatgc gtcgtttaga gcagcagccg aattaattct
     5341 agttccagtg aaatccaagc attttctaaa ttaaatgtat tcttattatt atagttgtta
     5401 tttttgatat atataaacaa cactattatg cccaccattt ttttgagatg catctacaca
     5461 aggaacaaac actggatgtc actttcagtt caaattgtaa cgctaatcac tccgaacagg
     5521 tcacaaaaaa ttaccttaaa aagtcataat attaaattag aataaatata gctgtgaggg
     5581 aaatatatac aaatatattg gagcaaataa attgtacata caaatattta ttactaattt
     5641 ctattgagac gaaatgaacc actcggaacc atttgagcga accgaatcgc gcggaactaa
     5701 cgacagtcgc tccaaggtcg tcgaacaaaa ggtgaatgtg ttgcggagag cgggtgggag
     5761 acagcgaaag agcaactacg aaacgtggtg tggtggaggt gaattatgaa gagggcgcgc
     5821 gatttgaaaa gtatgtatat aaaaaatata tcccggtgtt ttatgtagcg ataaacgagt
     5881 ttttgatgta aggtatgcag gtgtgtaagt cttttggtta gaagacaaat ccaaagtcta
     5941 cttgtgggga tgttcgaagg ggaaatactt gtattctata ggtcatatct tgtttttatt
     6001 ggcacaaata taattacatt agctttttga gggggcaata aacagtaaac acgatggtaa
     6061 taatggtaaa aaaaaaaaca agcagttatt tcggatatat gtcggctact ccttgcgtcg
     6121 ggcccgaagt cttagagcca gatatgcgag cacccggaag ctcacgatga gaatggccag
     6181 acccacgtag tccagcggca gatcggcggc ggagaagtta agcgtctcca ggatgacctt
     6241 gcccgaactg gggcacgtgg tgttcgacga tgtgcagcta atttcgcccg gctccacgtc
     6301 cgcccattgg ttaatcagca gaccctcgtt ggcgtaacgg aaccatgaga ggtacgacaa
     6361 ccatttgagg tatactggca ccgagcccga gttcaagaag aagccgccaa agagcaggaa
     6421 tggtatgata accggcggac ccacagacag cgccatcgag gtcgaggagc tggcgcagga
     6481 tattagatat ccgaaggacg ttgacacatt ggccaccaga gtgaccagcg ccaggcagtt
     6541 gaagaagtgc agcactccgg cccgcagtcc gatcatcgga taggcaatcg ccgtgaagac
     6601 cagtggcact gtgagaaaaa gcggtaattc ggcaatcgtt ttgcccagaa agtatgtgtc
     6661 acagcgataa agtcgacttc gggcctccct cataaaaact ggcagctctg aggtgaacac
     6721 ctaaatcgaa tcgattcatt agaaagttag taaattatta atatgcaaat gtattctaaa
     6781 caagacttac atttatcgtg gcaaagacgt tttgaaaggt catgttggtc aggaagagga
     6841 agatggctcc gttgatattc atcacgccca cttgcgtgag ttgttggccc aaaaagatga
     6901 ggccaatcaa gatggcaacc atctgcaaat taaaatgtta ctcgcatctc attaatattc
     6961 atatcttcaa catgttcgcg agttaaatga aatttattta ttttctgcaa aactataaac
     7021 tatacatctc attgaaaaaa actaagaagg gtgtggaatc aggcaattct aactaaaatc
     7081 tagcgaattt gtttccaaga attgtaagcg ttatatcatt tgtttccact ggaaccactc
     7141 accgttgtct gaataagtcg cacttttacg aggagtggtt ccttgagcac cgacagccag
     7201 gatcgccaca ggaccgcccg gaactgcatg aaccaggtgg ccttgtaggt gtacccattc
     7261 tccggctgct ccagtggctt ctccaaattt ttggtggcca acaactgctc catatcccgg
     7321 gctactttgc taatagcaaa attgtcgcca tatcttggcg atccgatcac gggactcgat
     7381 ctcccgtccg ggcacaacgg ccaacacctg tacgtaaaag tccgccggat tgtagttggt
     7441 aggacactgg gcacccacgc tggataggag ttgagatgta atgtaatgct agataccctt
     7501 aataaacaca tcgaactcac taggaaaaga agtcgacggc ttcgctggga gtgcccaaga
     7561 aagctaccct gccctcggcc atcagaagga tcttgtcaaa gagctcaaac agctcggaag
     7621 acggctgatg aatggtcagg atgacggtct tgcccttctg cgacagcttc ttcagcacct
     7681 ggacgacgct gtgggcggta aatgagtcca gtccggaggt gggctcatcg cagatcagaa
     7741 gcggcggatc ggttagtgcc tcggaggcga atgccagacg cttcctttct ccgccggaca
     7801 gacctttcac cctgccgggc acaccgatga tcgtgtgctg acatttgctg agcgaaagct
     7861 cctggatcac ctgatccacg cgggccactc gctgccgata ggtcagatgt cgtggcatcc
     7921 gcaccatggc ctggaaaatc aggtgttccc tggccgttag ggagccgata aagaggtcat
     7981 cctgctggac ataggcgcac ctggcctgca tctccttggc gtccacaggt tggccattga
     8041 gcagtcgcat cccggatggc gatacttgga tgccctgcgg cgatcgaaag gcaagggcat
     8101 tcagcagggt cgtctttccg gcaccggaac tgcccatcac ggccaaaagt tcgcccggat
     8161 aggccacgcc gcaaactgag tttcaaattg gtaattggac cctttattaa gatttcacac
     8221 agatcagccg actgcgaata gaaactcacc gttcttgagc aaatgtttcc tgggcgccgg
     8281 tatgtgtcgc tcgttgcaga atagtccgcg tgtccggttg accagctgcc gccatccgga
     8341 gcccggctga ttgaccgccc caaagatgtc catattgtgc caggcatagg tgaggttctc
     8401 ggctagttgg ccgctccctg aaccggagtc ctccggcgga ctgggtggca ggagcgtgcc
     8461 gtagtttttg gcctgcccga agccctggtt aatgcagctc tgcgaagcgt ccgctgtcac
     8521 cctgcaatga taggggatct caaatatcaa ctacaagcgt tatgctcatc taaccccgaa
     8581 caaaacgaag tatcctacga agtaggttta tacttttatt tattttttgt gcatctagga
     8641 tcagcttaaa atatctggtt gttatatttt ttgtaaaaaa gaatgtagtc gaaaatgaat
     8701 gcctttagat gtcttgatca tgatatgatc ttaaaaattg tcttatatag cgagcacagc
     8761 taccagaata atctgtttcg tgtcactatt tgtttgtgcg attgcggttt gggatttttg
     8821 tgggtcgcag ttctcacgcc gcagacaatt tgatgttgca atcgcagttc ctatagatca
     8881 agtgaactta agatgtatgc acatgtacta ctcacattgt tcagatgctc ggcagatggg
     8941 tgtttgctgc ctccgcgaat taatagctcc tgatcctctt ggcccattgc cgggattttt
     9001 cacactttcc cctgcttacc cacccaaaac caatcaccac cccaatcact caaaaaacaa
     9061 acaaaaataa gaagcgagag gagttttggc acagcacttt gtgtttaatt gatggcgtaa
     9121 accgcttgga gcttcgtcac gaaaccgctg acaaagtgca actgaaggcg gacattgacg
     9181 ctaggtaacg ctacaaacgg tggcgaaaga gatagcggac gcagcggcga aagagacggc
     9241 gatatttctg tggacagaga aggaggcaaa cagcgctgac tttgagtgga atgtcatttt
     9301 gagtgagagg taatcgaaag aacctggtac ttcaaatacc cttggatcga agtaaattta
     9361 aaactgatca gataagttca atgatatcca gtgcagtaaa aaataaaaaa aaaatatgtt
     9421 tttttaaatc tacattctcc aaaaaagggt tttattaact tacatacata ctagaattgg
     9481 ccgctctagc ccccctcgaa tgttctctct cttctcttct ctctctcttt ctcgaatgtt
     9541 ctctctcttc tcttctctct ctctttctcg aggtcatcaa gcttaggcct ccaaggcgga
     9601 gtactgtcct ccgggctggc ggagtactgt cctccggcaa ggtcggagta ctgtcctccg
     9661 acactagagg tcggagtact gtcctccgac gcaaggtcgg agtactgtcc tccgacacta
     9721 gaggtcggag tactgtcctc cgacgcaagg tcggagtact gtcctccgac actagaggtc
     9781 ggagtactgt cctccgacgc aaggcggagt actgtcctcc gggctggcgg agtactgtcc
     9841 tccggcaagg tcggagtact gtcctccgac actagaggtc ggagtactgt cctccgacgc
     9901 aaggcggagt actgtcctcc gggctggcgg agtactgtcc tccggcaagg gtcgagtcga
     9961 tagccgaagc ttaccgaagt atacacttaa attcagtgca cgtttgcttg ttgagaggaa
    10021 aggttgtgtg cggacgaatt tttttttgaa aa
//
