Basic Vector Information
- Vector Name:
- pUASg.attB
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10293 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K.
- Promoter:
- hsp70
pUASg.attB vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUASg.attB vector Sequence
LOCUS 40924_44699 10293 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pUASg.attB, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10293) AUTHORS Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K. TITLE A versatile platform for creating a comprehensive UAS-ORFeome library in Drosophila JOURNAL Development (2013) In press PUBMED 23637332 REFERENCE 2 (bases 1 to 10293) AUTHORS Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K. TITLE Direct Submission JOURNAL Submitted (13-APR-2013) Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland REFERENCE 3 (bases 1 to 10293) TITLE Direct Submission REFERENCE 4 (bases 1 to 10293) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Development (2013) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (13-APR-2013) Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..10293 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(3..458) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 600..616 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 623..641 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" gene 666..4802 /label=mini-white /note="This modified version of the white gene lacks part of the first intron." protein_bind 4842..4936 /label=5X UAS /note="five tandem copies of the 'ScaI site' 17-mer CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) that efficiently binds yeast Gal4 (Webster et al., 1988; Pfeiffer et al., 2010)" promoter 4955..5193 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter" protein_bind 5236..5360 /label=attR1 /note="recombination site for the Gateway(R) LR reaction" promoter 5385..5415 /label=lac UV5 promoter /note="E. coli lac promoter with an 'up' mutation" CDS 5469..6146 /label=CmR /note="chloramphenicol acetyltransferase" CDS 6469..6771 /label=ccdB /note="CcdB, a bacterial toxin that poisons DNA gyrase" protein_bind complement(6815..6939) /label=attR2 /note="recombination site for the Gateway(R) LR reaction" regulatory 6953..7765 /label=tubulin alpha 1 terminator /note="tubulin alpha 1 terminator" /regulatory_class="terminator" protein_bind 7817..7886 /label=attB /note="attB site for the phi-C31 integrase (Groth et al., 2000)" promoter complement(8105..8123) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(8144..8160) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 8168..8184 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8192..8222) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(8237..8258) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(8546..9134) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9308..10165) /label=AmpR /note="beta-lactamase" promoter complement(10166..10270) /label=AmpR promoter
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