LOCUS       40924_44699       10293 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUASg.attB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K.
  TITLE     A versatile platform for creating a comprehensive UAS-ORFeome 
            library in Drosophila
  JOURNAL   Development (2013) In press
  PUBMED    23637332
REFERENCE   2  (bases 1 to 10293)
  AUTHORS   Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2013) Institute of Molecular Life Sciences, 
            University of Zurich, Winterthurerstrasse 190, Zurich 8057, 
            Switzerland
REFERENCE   3  (bases 1 to 10293)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10293)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Development
            (2013) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2013) Institute of Molecular Life Sciences, University of 
            Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4842..4936
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        4955..5193
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    5236..5360
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5385..5415
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             5469..6146
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             6469..6771
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(6815..6939)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      6953..7765
                     /label=tubulin alpha 1 terminator
                     /note="tubulin alpha 1 terminator"
                     /regulatory_class="terminator"
     protein_bind    7817..7886
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(8105..8123)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8144..8160)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8168..8184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8192..8222)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8237..8258)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8546..9134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9308..10165)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10166..10270)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagagc ttaggattta aatgcttgca tgcctgcagg tcggagtact gtcctccgag
     4861 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
     4921 agtactgtcc tccgagcgga gactctagcg agcgccggag tataaataga ggcgcttcgt
     4981 ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa
     5041 gcaaataaac aagcgcagct gaacaagcta aacaatctgc agtaaagtgc aagttaaagt
     5101 gaatcaatta aaagtaacca gcaaccaagt aaatcaactg caactactga aatctgccaa
     5161 gaagtaatta ttgaatacaa gaagagaact ctgaataggg aattgggaat tcgttaacag
     5221 atctgctacc ggtatacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
     5281 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
     5341 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt
     5401 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg
     5461 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
     5521 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     5581 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     5641 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
     5701 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     5761 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     5821 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     5881 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
     5941 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     6001 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     6061 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcaggcgg
     6121 ggcgtaatct agaggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
     6181 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
     6241 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
     6301 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
     6361 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
     6421 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag
     6481 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
     6541 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
     6601 aaagtccccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
     6661 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
     6721 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     6781 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt
     6841 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     6901 attttacgtt tctcgttcag ctttcttgta caaagtggtg acgtaagcta gcactaagcg
     6961 tcgcgccact tcaacgctcg atgggagcgt cattggtggg cggggtaacc gtcgaaatca
     7021 gtgtttacgc ttccaatcgc aacaaaaaat tcactgcaac actgaaaagc atacgaaaac
     7081 gatgaagatt gtacgagaaa ccataaagta ttttatccac aaagacacgt atagcagaaa
     7141 agccaagtta actcggcgat aagttgtgta cacaagaata aaatcggcca gattcagtgt
     7201 tgtcagaaat aagaaaaccc cactatgttt ttctttgcct tttctttctc ccagcgatca
     7261 ttcatttcgt ggtgaaagaa cggggtcatt gcacggagtt tcgactgcgg gaaagcagag
     7321 ctgccgttca cttcgtctat aattagcgct ttctattttc cccgattcgg gccgctgctg
     7381 cgcttttccg cctgctgttt gtggcaagtg tagcagcagg ctgtgcacgc agtgtggcat
     7441 gcacttggct ttccaccgtt ggtatcgatt ctctgggacg atgagtcatt cctttcgggg
     7501 ccacagcata atcgttgcca gctcaccgaa atggtgactt catttcttaa ctgccgtcaa
     7561 gcatgcgatt gtacatacat acatatttat atatgtacat atttatgtga ctatggtagg
     7621 tcgatataat agcaatcaac gcaagcaaat gtgtcagtcc tgcttacagg aacgattcta
     7681 tttagtaatt ttcgttgtat aaagtaatta tgtatgtatg taagccccat aaatctgaaa
     7741 caattaggca aaaccatgcg aagctctcga gctcggtaag cttagatcca ctagtgtcga
     7801 cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc ttgggctccc
     7861 cgggcgcgta ctccacctca cccatctggt ccatcatgat gaacgggtcg aggtggcggt
     7921 agttgatccc ggcgaacgcg cggcgcaccg ggaagccctc gccctcgaaa ccgctgggcg
     7981 cggtggtcac ggtgagcacg ggacgtgcga cggcgtcggc gggtgcggat acgcggggca
     8041 gcgtcagcgg gttctcgacg gtcacggcgg gcatgtcgac actagttcta gccagctttt
     8101 gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg
     8161 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     8221 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     8281 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     8341 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8401 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8461 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8521 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8581 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8641 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8701 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8761 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8821 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8881 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8941 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9001 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9061 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9121 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9181 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9241 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9301 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9361 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9421 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9481 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9541 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9601 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9661 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9721 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9781 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9841 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9901 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9961 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10021 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10081 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10141 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10201 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10261 gggttccgcg cacatttccc cgaaaagtgc cac
//
