Basic Vector Information
pUAS-NanoLuc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUAS-NanoLuc vector Sequence
LOCUS 40924_44689 3485 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pUAS-NanoLuc, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3485)
AUTHORS Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu
S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell
RE.
TITLE Optogenetic control with a photocleavable protein, PhoCl
JOURNAL Nat. Methods (2017) In press
PUBMED 28288123
REFERENCE 2 (bases 1 to 3485)
AUTHORS Zhang W.
TITLE Direct Submission
JOURNAL Submitted (01-FEB-2017) Chemistry, University of Alberta, 11227
Saskatchewan Drive, Department of Chemistry, CCIS 4-140, Edmonton,
Alberta T6G2C5, Canada
REFERENCE 3 (bases 1 to 3485)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3485)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
Methods (2017) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(01-FEB-2017) Chemistry, University of Alberta, 11227 Saskatchewan
Drive, Department of Chemistry, CCIS 4-140, Edmonton, Alberta
T6G2C5, Canada"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Assembly Method :: GeneStudio v. 2.2.0.0
Sequencing Technology :: Illumina
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..3485
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 85..179
/label=5X UAS
/note="five tandem copies of the 'ScaI site' 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
promoter 198..436
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
CDS 481..993
/codon_start=1
/label=Nluc
/note="NanoLuc(R) luciferase"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
polyA_signal complement(1039..1160)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1579..2167)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2370..3227)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 3332..3380
/label=poly(A) signal
/note="synthetic polyadenylation signal"
misc_feature 3394..3485
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
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