LOCUS       40924_44689        3485 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUAS-NanoLuc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu 
            S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell
            RE.
  TITLE     Optogenetic control with a photocleavable protein, PhoCl
  JOURNAL   Nat. Methods (2017) In press
  PUBMED    28288123
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Zhang W.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2017) Chemistry, University of Alberta, 11227 
            Saskatchewan Drive, Department of Chemistry, CCIS 4-140, Edmonton, 
            Alberta T6G2C5, Canada
REFERENCE   3  (bases 1 to 3485)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3485)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-FEB-2017) Chemistry, University of Alberta, 11227 Saskatchewan 
            Drive, Department of Chemistry, CCIS 4-140, Edmonton, Alberta 
            T6G2C5, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: GeneStudio v. 2.2.0.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    85..179
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        198..436
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             481..993
                     /codon_start=1
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     polyA_signal    complement(1039..1160)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1579..2167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2370..3227)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3332..3380
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3394..3485
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg
      121 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
      181 ggagactcta gcgagcgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc
      241 aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca
      301 gctgaacaag ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa
      361 ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata
      421 caagaagaga actctgaata gggaattggg aattcgttaa cagatccgat atccgccacc
      481 atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg
      541 gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta
      601 actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc
      661 atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag
      721 gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta
      781 atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc
      841 gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc
      901 gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg
      961 accggctggc ggctgtgcga acgcattctg gcgtaataat ctagagtcgg ggcggccggc
     1021 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     1081 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     1141 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     1201 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
     1261 aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     1321 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     1381 caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1441 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1501 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1561 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1621 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1681 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1741 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1801 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1861 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1921 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1981 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2041 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2101 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2161 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagcgg
     2341 ccgcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc accgatctca
     2401 gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta gatcactacg
     2461 attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga gccgcgttca
     2521 ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg aagaagtggt
     2581 cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc tagagtaaga
     2641 agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat cgtggtatca
     2701 cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag ccgggtcaca
     2761 tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat cgttgtcaga
     2821 agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa ttctcttacc
     2881 gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa gtcgttttgt
     2941 gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga caacaccgcg
     3001 ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg gcggaaagac
     3061 tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc acccagttga
     3121 tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg caagcaaaat
     3181 gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact cgtccttttt
     3241 caatattatt gaagcattta tcagggttac tagtacgtct ctcaaggata agtaagtaat
     3301 attaaggtac gggaggtatt ggacaggccg caataaaata tctttatttt cattacatct
     3361 gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac
     3421 gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt
     3481 tctct
//
