Basic Vector Information
pUAS-K10attB vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUAS-K10attB vector Sequence
LOCUS 40924_44679 9537 bp DNA circular SYN 18-DEC-2018
DEFINITION Transformation vector pUAS-K10attB, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9537)
AUTHORS Koch R, Ledermann R, Urwyler O, Heller M, Suter B.
TITLE Systematic functional analysis of Bicaudal-D serine phosphorylation
and intragenic suppression of a female sterile allele of BicD
JOURNAL PLoS ONE 4 (2), E4552 (2009)
PUBMED 19234596
REFERENCE 2 (bases 1 to 9537)
AUTHORS Koch R, Ledermann R, Urwyler O, Heller M, Suter B.
TITLE Direct Submission
JOURNAL Submitted (19-MAY-2008) Institute for Cell Biology, University of
Berne, Baltzerstrasse 4, Bern 3012, Switzerland
REFERENCE 3 (bases 1 to 9537)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9537)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2009"; volume: "4"; issue: "2"; pages: "E4552"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(19-MAY-2008) Institute for Cell Biology, University of Berne,
Baltzerstrasse 4, Bern 3012, Switzerland"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9537
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 4..459
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 623..641
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
gene 666..4802
/label=mini-white
/note="This modified version of the white gene lacks part
of the first intron."
protein_bind 4809..4842
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
misc_feature 4854..4915
/label=contains 2 GAGA sites
/note="contains 2 GAGA sites"
misc_feature 4952..5298
/label=UAS Gal4 binding sites
/note="UAS Gal4 binding sites"
regulatory 5310..5554
/label=P-transposase promoter
/note="P-transposase promoter"
/regulatory_class="promoter"
misc_feature 5536..5598
/label=multiple cloning site
/note="multiple cloning site"
misc_feature 5648..7028
/label=K10 polyA signal sequence
/note="K10 polyA signal sequence"
protein_bind 7061..7130
/label=attB
/note="attB site for the phi-C31 integrase (Groth et al.,
2000)"
promoter complement(7349..7367)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(7388..7404)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(7412..7428)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7436..7466)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(7481..7502)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(7790..8378)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8552..9409)
/label=AmpR
/note="beta-lactamase"
promoter complement(9410..9514)
/label=AmpR promoter
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