LOCUS       40924_44679        9537 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pUAS-K10attB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Koch R, Ledermann R, Urwyler O, Heller M, Suter B.
  TITLE     Systematic functional analysis of Bicaudal-D serine phosphorylation 
            and intragenic suppression of a female sterile allele of BicD
  JOURNAL   PLoS ONE 4 (2), E4552 (2009)
  PUBMED    19234596
REFERENCE   2  (bases 1 to 9537)
  AUTHORS   Koch R, Ledermann R, Urwyler O, Heller M, Suter B.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2008) Institute for Cell Biology, University of 
            Berne, Baltzerstrasse 4, Bern 3012, Switzerland
REFERENCE   3  (bases 1 to 9537)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9537)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2009"; volume: "4"; issue: "2"; pages: "E4552"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-MAY-2008) Institute for Cell Biology, University of Berne, 
            Baltzerstrasse 4, Bern 3012, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4809..4842
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4854..4915
                     /label=contains 2 GAGA sites
                     /note="contains 2 GAGA sites"
     misc_feature    4952..5298
                     /label=UAS Gal4 binding sites
                     /note="UAS Gal4 binding sites"
     regulatory      5310..5554
                     /label=P-transposase promoter
                     /note="P-transposase promoter"
                     /regulatory_class="promoter"
     misc_feature    5536..5598
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    5648..7028
                     /label=K10 polyA signal sequence
                     /note="K10 polyA signal sequence"
     protein_bind    7061..7130
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(7349..7367)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7388..7404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7412..7428)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7436..7466)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7481..7502)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7790..8378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8552..9409)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9410..9514)
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcga tctaattggc
     4861 cgctctagcc ccccctcgaa tgttctctct cttctcttct ctctctcttt ctcgaatgtt
     4921 ctctctcttc tcttctctct ctctttctcg aggtcatcaa gcttaggcct ccaaggcgga
     4981 gtactgtcct ccgggctggc ggagtactgt cctccggcaa ggcggagtac tgtcctccgg
     5041 gctggcggag tactgtcctc cggcaaggcg gagtactgtc ctccgggctg gcggagtact
     5101 gtcctccggc aaggcggagt actgtcctcc gggctggcgg agtactgtcc tccggcaagg
     5161 cggagtactg tcctccgggc tggcggagta ctgtcctccg gcaaggcgga gtactgtcct
     5221 ccgggctggc ggagtactgt cctccggcaa ggcggagtac tgtcctccgg gctggcggag
     5281 tactgtcctc cggcaagggt cgagtcgata gccgaagctt accgaagtat acacttaaat
     5341 tcagtgcacg tttgcttgtt gagaggaaag gttgtgtgcg gacgaatttt tttttgaaaa
     5401 ccggtgatag agcctgaacc agaaaagata aaagaaggct ataccagtgg gagtacacaa
     5461 acagagtaag tttgaatagt aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt
     5521 taggtcctgt tcattggtac ccgcccgggg atcagatccg cggccgcata ggccactagt
     5581 ggatctggat cctctagagt cgacctcgaa cgttaacgtt aacgtaacgt taactcgagg
     5641 agcttgataa cattatacct aaacccatgg tcaagagtaa acatttctgc ctttgaagtt
     5701 gagaacacaa ttaagcatcc cctggttaaa cctgacattc atacttgtta atagcgccat
     5761 aaacatagca ccaatttcga agaaatcagt taaaagcaat tagcaattag caattagcaa
     5821 taactctgct gacttcaaaa cgagaagagt tgcaagtatt tgtaaggcac agtttataga
     5881 ccaccgacgg ctcattaggg ctcgtcatgt aactaagcgc ggtgaaaccc aattgaacat
     5941 atagtggaat tattattatc aatggggaag atttaaccct caggtagcaa agtaatttaa
     6001 ttgcaaatag agagtcctaa gactaaataa tatatttaaa aatctggccc tttgaccttg
     6061 cttgtcaggt gcatttgggt tcaatcgtaa gttgcttcta tataaacact ttccccatcc
     6121 ccgcaataat gaagaatacc gcagaataaa gagagatttg caacaaaaaa taaaggcatt
     6181 gcgaaaactt tttatggggg atcattacac tcgggcctac ggttacaatt cccagccact
     6241 taagcgacaa gtttggccaa caatccatct aatagctaat agcgcaatca ctggtaatcg
     6301 caagagtata taggcaatag aacccatgga tttgaccaaa ggtaaccgag acaatggaga
     6361 agcaagagga tttcaaactg aacacccaca gtgctgtgta ctaccactgg cgcgtttggg
     6421 agctcactgg cctgatgcgt cctccgggcg tttcaagcct gctttacgtg gtatactcca
     6481 ttacggtcaa cttggtggtc accgtgctgt ttcccttgag cttgctggcc aggctgctgt
     6541 tcaccaccaa catggccgga ttgtgcgaga acctgaccat aactattacc gatattgtgg
     6601 ccaatttgaa gtttgcgaat gtgtacatgg tgaggaagca gctccatgag attcgctctc
     6661 tcctaaggct catggacgct agagcccggc tggtgggcga tcccgaggag atttctgcct
     6721 tgaggaagga agtgaatatc gcacagggca ctttccgcac ctttgccagt attttcgtat
     6781 ttggcactac tttgagttgc gtccgcgtgg tcgttcgccc ggatcgagag ctcctgtatc
     6841 cggcctggtt cggcgttgac tggatgcact ccaccagaaa ctatgtgctc atcaatatct
     6901 accagctctt cggcttgata gtgcaggcta tacagaactg cgctagtgac tcctatccgc
     6961 ctgcgtttct ctgcctgctc acgggtcata tgcgtgcttt ggagctgagg gtgcggcgga
     7021 ttggctgaga tccactagtg tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt
     7081 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca
     7141 tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc accgggaagc
     7201 cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt
     7261 cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt
     7321 cgacactagt tctagccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt
     7381 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7441 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7501 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7561 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7621 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7681 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7741 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7801 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7861 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7921 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7981 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8041 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8101 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8161 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8221 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8281 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8341 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8401 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8461 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8521 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8581 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8641 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8701 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8761 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8821 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8881 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8941 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9001 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9061 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9121 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9181 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9241 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9301 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9361 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9421 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9481 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
