pTZ110 vector (V002485)

LOCUS       40924_44614        7856 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTZ110, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Schweizer HP, Chuanchuen R.
  TITLE     Small broad-host-range lacZ operon fusion vector with low background
            activity
  JOURNAL   BioTechniques 31 (6), 1258 (2001)
  PUBMED    11768652
REFERENCE   2  (bases 1 to 7856)
  AUTHORS   Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2001) Microbiology, Colorado State University, 
            Fort Collins, CO 80523, USA
REFERENCE   3  (bases 1 to 7856)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7856)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2001"; volume: "31"; issue: "6"; pages: 
            "1258"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-MAY-2001) Microbiology, Colorado State University, Fort Collins,
            CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..36
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    37..47
                     /label=contains stop codons in all reading frames
                     /note="contains stop codons in all reading frames"
     regulatory      48..52
                     /gene="lacZ"
                     /label=modified
                     /note="modified"
                     /regulatory_class="ribosome_binding_site"
     CDS             70..3114
                     /label=lacZ
                     /note="beta-galactosidase"
     oriT            3550..3658
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(3711..4062)
                     /direction=LEFT
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             4076..4906
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     CDS             5413..5601
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5706..5846
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6032..6620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6794..7651)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7652..7756)
                     /label=AmpR promoter