Basic Vector Information
pTZ110 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTZ110 vector Sequence
LOCUS 40924_44614 7856 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pTZ110, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7856) AUTHORS Schweizer HP, Chuanchuen R. TITLE Small broad-host-range lacZ operon fusion vector with low background activity JOURNAL BioTechniques 31 (6), 1258 (2001) PUBMED 11768652 REFERENCE 2 (bases 1 to 7856) AUTHORS Schweizer HP. TITLE Direct Submission JOURNAL Submitted (03-MAY-2001) Microbiology, Colorado State University, Fort Collins, CO 80523, USA REFERENCE 3 (bases 1 to 7856) TITLE Direct Submission REFERENCE 4 (bases 1 to 7856) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "BioTechniques"; date: "2001"; volume: "31"; issue: "6"; pages: "1258" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (03-MAY-2001) Microbiology, Colorado State University, Fort Collins, CO 80523, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..7856 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..36 /label=multiple cloning site /note="multiple cloning site" misc_feature 37..47 /label=contains stop codons in all reading frames /note="contains stop codons in all reading frames" regulatory 48..52 /gene="lacZ" /label=modified /note="modified" /regulatory_class="ribosome_binding_site" CDS 70..3114 /label=lacZ /note="beta-galactosidase" oriT 3550..3658 /label=oriT /note="incP origin of transfer" rep_origin complement(3711..4062) /direction=LEFT /label=pRO1600 oriV /note="broad-host-range origin of replication from Pseudomonas aeruginosa plasmid pRO1600; requires the pRO1600 Rep protein for replication (West et al., 1994)" CDS 4076..4906 /label=pRO1600 Rep /note="replication protein for the broad-host-range plasmid pRO1600 from Pseudomonas aeruginosa" CDS 5413..5601 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 5706..5846 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(6032..6620) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6794..7651) /label=AmpR /note="beta-lactamase" promoter complement(7652..7756) /label=AmpR promoter
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