pTZ110 vector (V002485)

Basic Vector Information

      • Vector Name:
      • pTZ110
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 7856 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Schweizer HP, Chuanchuen R.

pTZ110 vector Vector Map

pTZ1107856 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066006900720075007800multiple cloning sitecontains stop codons in all reading framesmodifiedlacZoriTpRO1600 oriVpRO1600 RepropbomoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTZ110 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_44614        7856 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTZ110, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Schweizer HP, Chuanchuen R.
  TITLE     Small broad-host-range lacZ operon fusion vector with low background
            activity
  JOURNAL   BioTechniques 31 (6), 1258 (2001)
  PUBMED    11768652
REFERENCE   2  (bases 1 to 7856)
  AUTHORS   Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2001) Microbiology, Colorado State University, 
            Fort Collins, CO 80523, USA
REFERENCE   3  (bases 1 to 7856)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7856)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2001"; volume: "31"; issue: "6"; pages: 
            "1258"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-MAY-2001) Microbiology, Colorado State University, Fort Collins,
            CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..36
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    37..47
                     /label=contains stop codons in all reading frames
                     /note="contains stop codons in all reading frames"
     regulatory      48..52
                     /gene="lacZ"
                     /label=modified
                     /note="modified"
                     /regulatory_class="ribosome_binding_site"
     CDS             70..3114
                     /label=lacZ
                     /note="beta-galactosidase"
     oriT            3550..3658
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(3711..4062)
                     /direction=LEFT
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             4076..4906
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     CDS             5413..5601
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5706..5846
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6032..6620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6794..7651)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7652..7756)
                     /label=AmpR promoter

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