LOCUS       40924_44614        7856 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTZ110, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Schweizer HP, Chuanchuen R.
  TITLE     Small broad-host-range lacZ operon fusion vector with low background
            activity
  JOURNAL   BioTechniques 31 (6), 1258 (2001)
  PUBMED    11768652
REFERENCE   2  (bases 1 to 7856)
  AUTHORS   Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2001) Microbiology, Colorado State University, 
            Fort Collins, CO 80523, USA
REFERENCE   3  (bases 1 to 7856)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7856)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2001"; volume: "31"; issue: "6"; pages: 
            "1258"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-MAY-2001) Microbiology, Colorado State University, Fort Collins,
            CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..36
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    37..47
                     /label=contains stop codons in all reading frames
                     /note="contains stop codons in all reading frames"
     regulatory      48..52
                     /gene="lacZ"
                     /label=modified
                     /note="modified"
                     /regulatory_class="ribosome_binding_site"
     CDS             70..3114
                     /label=lacZ
                     /note="beta-galactosidase"
     oriT            3550..3658
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(3711..4062)
                     /direction=LEFT
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             4076..4906
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     CDS             5413..5601
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5706..5846
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6032..6620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6794..7651)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7652..7756)
                     /label=AmpR promoter
ORIGIN
        1 gaattcaggc ctaagcttct cgagcccggg ggatcctaag taagtaagga gaaaaaaatg
       61 gctgatcccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      121 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      181 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
      241 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
      301 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtaacctat
      361 cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc
      421 acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc
      481 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt
      541 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg
      601 gtgatggtgc tgcgttggag tgacggcagt tatctggaag atcaggatat gtggcggatg
      661 agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc
      721 catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag
      781 atgtgcggcg agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg
      841 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat
      901 gccgatcgcg tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc
      961 ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca
     1021 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     1081 ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag
     1141 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     1201 gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac
     1261 ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     1321 ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag
     1381 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc
     1441 gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag
     1501 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     1561 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
     1621 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
     1681 cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt atccccgttt acagggcggc
     1741 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg
     1801 tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt
     1861 ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag
     1921 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     1981 catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc
     2041 ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta
     2101 ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg
     2161 accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac
     2221 ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg
     2281 gatttttgca tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt
     2341 tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
     2401 cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc
     2461 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc
     2521 acggcagata cacttgctga tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
     2581 gggaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
     2641 attaccgttg atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgaac
     2701 tgccagctgg cgcaggtagc agagcgggta aactggctcg gattagggcc gcaagaaaac
     2761 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
     2821 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
     2881 tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta cagtcaacag
     2941 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
     3001 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg
     3061 gatttccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa
     3121 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
     3181 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatggga
     3241 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
     3301 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
     3361 ctgctttctg acaaactcgg gcagcgttgg gtcctggggg attccttaag gtatactttc
     3421 cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt
     3481 cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg
     3541 gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac
     3601 tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgat
     3661 aagctcctct caggcgccgc tggtgccgct ggttggacgc caagggtgaa tccgcctcga
     3721 taccctgatt actcgcttcc tgcgccctct caggcggcga taggggactg gtaaaacggg
     3781 gattgcccag acgcctcccc cgccccttca ggggcacaaa tgcggcccca acggggccac
     3841 gtagtggtgc gttttttgcg tttccaccct tttcttcctt ttccctttta aaccttttag
     3901 gacgtctaca ggccacgtaa tccgtggcct gtagagttta aaaagggacg gatttgttgc
     3961 cattaaggga cggatttgtt gttaagaagg gacggatttg ttgttgtaaa gggacggatt
     4021 tgttgtattg tgggacgcag atacagtgtc cccttataca caaggaatgt cgaacgtggc
     4081 ctcaccccca atggtttaca aaagcaatgc cctggtcgag gccgcgtatc gcctcagtgt
     4141 tcaggaacag cggatcgttc tggcctgtat tagccaggtg aagaggagcg agcctgtcac
     4201 cgatgaagtg atgtattcag tgacggcgga ggacatagcg acgatggcgg gtgtccctat
     4261 cgaatcttcc tacaaccagc tcaaagaagc ggccctgcgc ctgaaacggc gggaagtccg
     4321 gttaacccaa gagcccaatg gcaaggggaa aagaccgagt gtgatgatta ccggctgggt
     4381 gcaaacaatc atctaccggg agggtgaggg ccgtgtagaa ctcaggttca ccaaagacat
     4441 gctgccgtac ctgacggaac tcaccaaaca gttcaccaaa tacgccttgg ctgacgtggc
     4501 caagatggac agcacccacg cgatcaggct ttacgagctg ctcatgcaat gggacagcat
     4561 cggccagcgc gaaatagaaa ttgaccagct gcgaaagtgg tttcaactgg aaggccggta
     4621 tccctcgatc aaggacttca agttgcgagt gcttgatcca gccgtgacgc agatcaacga
     4681 gcacagcccg ctacaggtgg agtgggcgca gcgaaagacc gggcgcaagg tcacacatct
     4741 gttgttcagt tttggaccga agaagcccgc caaggcggtg ggtaaggccc cagcgaagcg
     4801 caaggccggg aagatttcag atgctgagat cgcgaaacag gctcgccctg gtgagacatg
     4861 ggaagcggcc cgcgctcgac taacccagat gccgctggat ctggcctaga ggccgtggcc
     4921 accacggccc ggcctgcctt tcccacgggt gcgcatgatc gtgctcctgt cgttgaggac
     4981 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
     5041 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
     5101 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt
     5161 ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac
     5221 gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag
     5281 ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt
     5341 gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatt cccccttaca
     5401 cggaggcatc aagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga
     5461 agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac
     5521 atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc
     5581 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     5641 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     5701 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     5761 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     5821 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     5881 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5941 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6001 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6061 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6121 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6181 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     6241 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6301 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6361 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6421 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     6481 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6541 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6601 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6661 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6721 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6781 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6841 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6901 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6961 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7021 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7081 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     7141 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7201 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7261 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7321 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7381 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     7441 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7501 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7561 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7621 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7681 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7741 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     7801 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaa
//
