pTYGi9 vector (V002486)

MSDS

Basic Vector Information

- - Vector Name:
  - pTYGi9
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 6898 bp
- - Type:
  - Integrative vector
- - Replication origin:
  - ori
- - Source/Author:
  - Tianyu Z.
- - Promoter:
  - T3

pTYGi9 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTYGi9 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_44609        6898 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integrative vector pTYGi9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Tianyu Z.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   2  (bases 1 to 6898)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6898)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-DEC-2013) Center for Infection "
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2804..3916
                     /gene="33"
                     /label=33
                     /note="Integrase from Mycobacterium phage L5. Accession#: 
                     P22884"
     misc_feature    4378..4405
                     /label=MTB dif
                     /note="MTB dif"
     CDS             4467..5462
                     /gene="hyg"
                     /label=hyg
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces 
                     hygroscopicus. Accession#: P09979"
     CDS             5521..6237
                     /label=EGFP
                     /note="enhanced GFP"
     promoter        complement(6255..6273)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6283..6299)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6441..6896
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"

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