LOCUS       40924_44609        6898 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integrative vector pTYGi9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Tianyu Z.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   2  (bases 1 to 6898)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6898)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-DEC-2013) Center for Infection "
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2804..3916
                     /gene="33"
                     /label=33
                     /note="Integrase from Mycobacterium phage L5. Accession#: 
                     P22884"
     misc_feature    4378..4405
                     /label=MTB dif
                     /note="MTB dif"
     CDS             4467..5462
                     /gene="hyg"
                     /label=hyg
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces 
                     hygroscopicus. Accession#: P09979"
     CDS             5521..6237
                     /label=EGFP
                     /note="enhanced GFP"
     promoter        complement(6255..6273)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6283..6299)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6441..6896
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     2221 gccgctctag aactagagtc gaccaccaag ggcaccatct ctgcttgggc caccccgttg
     2281 gccgcagcca gctcgctgag agccgtgaac gacagggcga acgccagccc gccgacggcg
     2341 agggttccga ccgctgcaac tcccggtgca accttgtccc ggtctattct cttcactgca
     2401 ccagctccaa tctggtgtga atgcccctcg tctgttcgcg caggcggggg gctctattcg
     2461 tttgtcagca tcgaaagtag ccagatcagg gatgcgttgc aaccgcgtat gcccaggtca
     2521 gaagagtcgc acaagagttg cagacccctg gaaagaaaaa tggccagagg gcgaaaacac
     2581 cctctgacca gcggagcggg cgacgggaat cgaacccgcg tagctagttt ggaagaatgg
     2641 gtgtctgccg accacatatg ggccggtcaa gataggtttt taccccctct cggctgcatc
     2701 ctctaagtgg aaagaaattg caggtcgtag aagcgcgttg aagcctgaga gttgcacagg
     2761 agttgcaacc cggtagcctt gttcacgacg agaggagacc tagttggcac gtcgcggatg
     2821 gggatcgctg aagactcagc gcagcgggag gatccaagcc tcatacgtca acccgcagga
     2881 cggtgtgagg tactacgcgc tgcagaccta cgacaacaag atggacgccg aagcctggct
     2941 cgcgggcgag aagcggctca tcgagatgga gacctggacc cctccacagg accgggcgaa
     3001 gaaggcagcc gccagcgcca tcacgctgga ggagtacacc cggaagtggc tcgtggagcg
     3061 cgacctcgca gacggcacca gggatctgta cagcgggcac gcggagcgcc gcatctaccc
     3121 ggtgctaggt gaagtggcgg tcacagagat gacgccagct ctggtgcgtg cgtggtgggc
     3181 cgggatgggt aggaagcacc cgactgcccg ccggcatgcc tacaacgtcc tccgggcggt
     3241 gatgaacaca gcggtcgagg acaagctgat cgcagagaac ccgtgccgga tcgagcagaa
     3301 ggcagccgat gagcgcgacg tagaggcgct gacgcctgag gagctggaca tcgtcgccgc
     3361 tgagatcttc gagcactacc ggatcgcggc atacatcctg gcgtggacga gcctccggtt
     3421 cggagagctg atcgagcttc gccgcaagga catcgtggac gacggcatga cgatgaagct
     3481 ccgggtgcgc cgtggcgctt cccgcgtggg gaacaagatc gtcgttggca acgccaagac
     3541 cgtccggtcg aagcgtcctg tgacggttcc gcctcacgtc gcggagatga tccgagcgca
     3601 catgaaggac cgtacgaaga tgaacaaggg ccccgaggca ttcctggtga ccacgacgca
     3661 gggcaaccgg ctgtcgaagt ccgcgttcac caagtcgctg aagcgtggct acgccaagat
     3721 cggtcggccg gaactccgca tccacgacct ccgcgctgtc ggcgctacgt tcgccgctca
     3781 ggcaggtgcg acgaccaagg agctgatggc ccgtctcggt cacacgactc ctaggatggc
     3841 gatgaagtac cagatggcgt ctgaggcccg cgacgaggct atcgctgagg cgatgtccaa
     3901 gctggccaag acctcctgaa acgcaaaaag cccccctccc aaggacactg agtcctaaag
     3961 aggggggttt cttgtcagta cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg
     4021 ctctgtgcgg agacctgggc accagccccg ccgccgccag gagcattgcc gttcccgcca
     4081 gctgagttct gttgtgcgcc gcctatgtag agctggtcgt tgtaggtccg atctccaggc
     4141 gactttccgg cgacgctgag gatgtcgatc acagagcctc cgggaccgcc ggttgcggtc
     4201 aaacctgacc atccgacagc ggacgccgtg gtgtttcctc cagggcctcc ggccttgcct
     4261 gagaatacag agccagctcc cgctgcgcct ccagctccga cgagcccggt gatcgtcttg
     4321 gtcgacctgc aggcatgcaa gcttgatatc gaattccata tgcccaagct tctcgagact
     4381 tgacataatg tcgcttatcg gcttaatcga tctagaccgg ccgtgcggaa ttaagccggc
     4441 ccgtaccctg tgaatagagg tccgctgtga cacaagaatc cctgttactt ctcgaccgta
     4501 ttgattcgga tgattcctac gcgagcctgc ggaacgacca ggagttctgg gagccgctgg
     4561 cccgccgagc cctggaggag ctcgggctgc cggtgccgcc ggtgctgcgg gtgcccggcg
     4621 agagcaccaa ccccgtactg gtcggcgagc ccggcccggt gatcaagctg ttcggcgagc
     4681 actggtgcgg tccggagagc ctcgcgtcgg agtcggaggc gtacgcggtc ctggcggacg
     4741 ccccggttcc ggtgccccgc ctcctcggcc gcggcgagct gcggcccggc accggagcct
     4801 ggccgtggcc ctacctggtg atgagccgga tgaccggcac cacctggcgg tccgcgatgg
     4861 acggcacgac cgaccggaac gcgctgctcg ccctggcccg cgaactcggc cgggtgctcg
     4921 gacggctgca cagggtgccg ctgaccggga acaccgtgct caccccccat tccgaggtct
     4981 tcccggaact gctgcgggaa cgccgcgcgg cgaccgtcga ggaccaccgc gggtggggct
     5041 acctctcgcc ccggctgctg gaccgcctgg aggactggct gccggacgtg gacacgctgc
     5101 tggccggccg cgaaccccgg ttcgtccacg gcgacctgca cgggaccaac atcttcgtgg
     5161 acctggccgc gaccgaggtc accgggatcg tcgacttcac cgacgtctat gcgggagact
     5221 cccgctacag cctggtgcaa ctgcatctca acgccttccg gggcgaccgc gagatcctgg
     5281 ccgcgctgct cgacggggcg cagtggaagc ggaccgagga cttcgcccgc gaactgctcg
     5341 ccttcacctt cctgcacgac ttcgaggtgt tcgaggagac cccgctggat ctctccggct
     5401 tcaccgatcc ggaggaactg gcgcagttcc tctgggggcc gccggacacc gcccccggcg
     5461 cctgatctag acccgggact tgacataatg tcgcttatcg gcttactcga gaagcttgcc
     5521 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     5581 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     5641 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     5701 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     5761 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     5821 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     5881 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     5941 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     6001 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     6061 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6121 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     6181 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     6241 ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     6301 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     6361 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6421 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6481 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     6541 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6601 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6661 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6721 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     6781 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6841 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttag
//
